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20,802 results

Showing: 2501 - 2550 of 20802 results

  6. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 for shRNA
    Available Since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24150-plasmid-map-sequence-id-376525

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  10. pRBC-sfGFP-150TAG

    Plasmid
    #174076
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,
    Depositor
    Ryan Mehl
    Article
    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15
    Insert
    Superfolder GFP
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    T7
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    174076-plasmid-map-sequence-id-345697

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  18. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87856-plasmid-map-sequence-id-175019

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  20. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  22. pAAV.GfaABC1D.PI.Lck-GFP.SV40 (AAV5)

    Viral Prep
    #105598-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA. GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Shigetomi et al J Gen Physiol. 2013 Apr 15.
    Promoter
    GfaABC1D
    Tags
    GFP
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  27. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV1)

    Viral Prep
    #105530-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  29. rtTA-N144

    Plasmid
    #66810
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance
    Depositor
    Andrew Yoo
    Article
    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi
    Insert
    Reverse tetracycline-controlled transactivator
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available Since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    66810-plasmid-map-sequence-id-418923

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  32. CMV-R-GECO1.2

    Plasmid
    #45494
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1.2
    Expression
    Mammalian
    Mutation
    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    45494-plasmid-map-sequence-id-123029

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  34. pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (AAV9)

    Viral Prep
    #124651-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (#124651). In addition to the viral particles, you will also receive purified pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 plasmid DNA. CamKIIa-driven expression of ChrimsonR fused to mScarlet fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Christopher Harvey
    Article
    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-
    Promoter
    CaMKII
    Tags
    mScarlet
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only

  37. pSMAL-CellTag-V1 (Concentrated Lentiviral Prep)

    Viral Prep
    #115643-LVC
    Purpose
    Ready-to-use Concentrated Lentiviral Prep particles produced from pSMAL-CellTag-V1 (#115643). In addition to the viral particles, you will also receive purified pSMAL-CellTag-V1 plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.&lt;/p&gt;</p>
    Depositor
    Samantha Morris
    Article
    Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:
    Promoter
    Minimal CMV
    Tags
    EGFP
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  48. PB-GFP-Gal8

    Plasmid
    #127191
    Purpose
    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Insert
    GFP-Gal8 (LGALS8 Human)
    Tags
    Gal8 is fused to the c-terminus of GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127191-plasmid-map-sequence-id-248359

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Showing: 2501 - 2550 of 20802 results