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Showing: 3301 - 3350 of 19980 results

  3. pAAV.CMV.ffLuciferase.SV40

    Plasmid
    #105532
    Purpose
    AAV expression of firefly luciferase from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105532-plasmid-map-sequence-id-202366

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  7. pSNAPf-N1

    Plasmid
    #58187
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138272-plasmid-map-sequence-id-273617

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  14. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid
    #108542
    Purpose
    Lentivirus vector expressing Luciferase with blasticidin resistance
    Depositor
    Javier Alcudia
    Inserts
    Luciferase
    Neomycin
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1apha and PGK
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108542-plasmid-map-sequence-id-208671

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  15. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103869-plasmid-map-sequence-id-199177

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  17. EGF-bio-His

    Plasmid
    #53340
    Purpose
    Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EGF (EGF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53340-plasmid-map-sequence-id-83070

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  19. pWPI

    Plasmid
    #12254
    Purpose
    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.
    Depositor
    Didier Trono
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EMCV IRES-EGFP cassette
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12254-plasmid-map-sequence-id-429661

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  25. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid
    #127368
    Purpose
    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127368-plasmid-map-sequence-id-246345

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  27. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid
    #44362
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    April 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    44362-plasmid-map-sequence-id-292479

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  29. p404TEF1

    Plasmid
    #15972
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15972-plasmid-map-sequence-id-8457

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  30. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

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  33. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV8)

    Viral Prep
    #50476-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  34. AAV phSyn1(S)-tdTomato-WPRE (AAV5)

    Viral Prep
    #51506-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from AAV phSyn1(S)-tdTomato-WPRE (#51506). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-tdTomato-WPRE plasmid DNA. hSyn-driven tdTomato expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do
    Promoter
    phSyn1
    Tags
    tdTomato
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  35. EFSp-GFP-YAP (5SA)

    Plasmid
    #174170
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    EFS
    Available since
    Sept. 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174170-plasmid-map-sequence-id-343725

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  42. pSBtet-RH

    Plasmid
    #60500
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60500-plasmid-map-sequence-id-97547

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  43. pAAV-FLEX-tdTomato (AAV2)

    Viral Prep
    #28306-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-EF1a-Con/Fon-GCaMP6M (AAV8)

    Viral Prep
    #137119-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EF1a-Con/Fon-GCaMP6M (#137119). In addition to the viral particles, you will also receive purified pAAV-EF1a-Con/Fon-GCaMP6M plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6M. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3301 - 3350 of 19980 results