29,729 results

1. pFA6a-mYFP-hphMX6

   Plasmid

   #105155

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu, Thomas Pollard

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mYFP-hphMX6
   

   Use

   Tags

   mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Chicken ß-actin

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmSod1-G93A-N’His

   Plasmid

   #117705

   Purpose

   mutation on stability/solubility of SOD1

   Depositor

   Elizabeth Fisher

   Insert

   Sod1 (Sod1 Mouse)
   

   Use

   Tags

   His tag with Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   G93A

   Promoter

   T7

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ptRNAfMet-A37

   Plasmid

   #196657

   Purpose

   Plasmid for overexpression of native initiator tRNA (A37)

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   tRNAfMet-A37 expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   metY native promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSIBR003

   Plasmid

   #177666

   Purpose

   SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pALOD4 non-binding mutant

   Plasmid

   #111027

   Purpose

   Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A, G501A, T502A, T503A, L5…

   Promoter

   T7

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJCC_039 FnCas12a

   Plasmid

   #171666

   Purpose

   For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMG051 MBP-β-catenin-His, GST-CK1α

   Plasmid

   #154072

   Purpose

   Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Inserts

   CTNNB1 (CTNNB1 Human)
   CSNK1A1 (CSNK1A1 )
   

   Use

   Tags

   GST, His6, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAD/HisB-iRFP720

   Plasmid

   #45460

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP720
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

   Promoter

   araBAD

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

    Plasmid

    #111259

    Purpose

    mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBx-Spas-sgRNA-Gm

    Plasmid

    #105232

    Purpose

    Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

    Plasmid

    #198292

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 5KO9 (HMCES)

    Plasmid

    #101877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HMCES (HMCES Human)
    

    Use

    Tags

    6x His (modified C-term tag is AENLYFQSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pHAGE2-TetOminiCMV-mCherry

    Plasmid

    #136615

    Purpose

    Dox-inducible lentivral vector expressing mCherry red fluorescent protein

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAraGFPCDF

    Plasmid

    #47516

    Purpose

    Contains PBAD::GFP reporter, used for heterodimer competition assay

    Depositor

    Bryan Berger

    Article

    Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

    Plasmid

    #105235

    Purpose

    Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Bacterial

    Mutation

    D10A, H599A

    Promoter

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid

    #22029

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant).

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKAR2-Br512g3.1

    Plasmid

    #171200

    Purpose

    Expresses Br512g3.1 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al medRxiv 2022.08.09.22278605

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (vHP47…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDUET-1-alpha-synuclein-mCerulean3-His6

    Plasmid

    #110061

    Purpose

    For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    6 x His and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLP-pTac-Tn5-chlorR

    Plasmid

    #199994

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPath-pTac-integrase

    Plasmid

    #199999

    Purpose

    Entry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQlinkH-PTPIP51(36-235)

    Plasmid

    #170533

    Purpose

    Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

    Depositor

    Byung Il Lee

    Article

    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

    Insert

    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-2T-RNAPII-CTD wt #14-HA

    Plasmid

    #160688

    Purpose

    Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

    Depositor

    Jeffry Corden

    Article

    Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

    Insert

    RNA Polymerase II CTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C terminal domain only

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28b-FOXP2_P6

    Plasmid

    #122649

    Purpose

    Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

    Depositor

    Hendrik Dietz

    Article

    Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

    Insert

    Homo sapiens forkhead box P2 (FOXP2 Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YWHAE

    Plasmid

    #39146

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAE (YWHAE Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pMEXC3H

    Plasmid

    #110080

    Purpose

    Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tol2-mpeg1-dendra2

    Plasmid

    #51462

    Purpose

    expresses photoconvertible dendra in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

    Insert

    mpeg-dendra
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mpeg1

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. His6_BcLOV4_mCherry_BamUK

    Plasmid

    #114596

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    His6 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBAD-Citron1

    Plasmid

    #134300

    Purpose

    Bacterial expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-cMSP1D1

    Plasmid

    #199768

    Purpose

    Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP1D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOPINVH

    Plasmid

    #26041

    Depositor

    Ray Owens

    Article

    Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

    Insert

    u-LacZ-KHHHHHH
    

    Use

    Tags

    KHHHHHH and u-phosphatase secretion leader

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pB1H2w2-zif268

    Plasmid

    #18045

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

    Insert

    zif268
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Zif268 is fused to the omega subunit of RNA polym…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28C-mCherry-YTHDF2

    Plasmid

    #204406

    Purpose

    E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX6P2-p53 (1-90) (72R)

    Plasmid

    #62081

    Purpose

    Expression of human p53 (TAD residues 1-90) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains TAD/PP residues 1-90, 72R

    Promoter

    tac

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pDD108

    Plasmid

    #119884

    Purpose

    Integration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    BbaJ23104_EYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHN767

    Plasmid

    #178884

    Purpose

    Expression of bacterial PNGase

    Depositor

    Kazuhiro Abe

    Insert

    Peptide:N-glycosidase F
    

    Use

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBB528

    Plasmid

    #27390

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    lacIq
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUC18T-mini-Tn7T

    Plasmid

    #64957

    Purpose

    mini-Tn7 base vector with transcriptional terminator and oriT

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pATmin-GG

    Plasmid

    #191352

    Purpose

    Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAP-BAC

    Plasmid

    #120229

    Purpose

    TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pBbA5c-MevT-MBI (JBEI-3085)

    Plasmid

    #87950

    Purpose

    the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    MevT-MBI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pHT-6xR.ads-PxylA-DI

    Plasmid

    #47386

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Inserts

    ads
    dxs
    idi
    

    Use

    Tags

    6xR and His

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac and PxylA

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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47. pENTR R4 ATCC LacZ GCTT

    Plasmid

    #173668

    Purpose

    Destination vector for Golden Gate Cloning assemblies, blue-white screening

    Depositor

    Eric Marois

    Article

    Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCOLA-Spec-EV

    Plasmid

    #212095

    Purpose

    (Empty Backbone) pCOLA-Spec Empty Vector

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge