29,740 results
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Plasmid#224215PurposeBacteroides genomic editing tool; aTc inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIYN3
Plasmid#197099PurposeTo express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.DepositorInsertTyrRS variant, Nap3 tRNA
UseTagsExpressionBacterialMutationAla70, Thr158, Ser159, Arg286 (TyrRS)PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK234
Plasmid#73324PurposeProduces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioCDepositorInsertsviolacein biosynthesis protein VioA
violacein biosynthesis protein VioB
violacein biosynthesis protein VioC
UseTagsExpressionBacterialMutationPromoterT7 and n/aAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbE7k-RFP
Plasmid#35315DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl
Plasmid#36400DepositorInsertCrkl (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET-jAspSnFR3
Plasmid#203458PurposejAspSnFR3 in T7 bacterial expression vectorDepositorInsertjAspSnFR3
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
ScSpe1 pET-20b(+)
Plasmid#117145PurposeExpresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21DepositorInsertSPE1
UseTagsHistidineExpressionBacterialMutationPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsRPS27a
Plasmid#69561PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertRPS27A (RPS27A Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-EGFP
Plasmid#177136PurposeBacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFPDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterTacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
6BHD (SETDB1)
Plasmid#119955PurposeBacterial expression for structure determinationDepositorInsertSETDB1 (SETDB1 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 190-410PromoterT7Available sinceFeb. 1, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18-mini-Tn7
Plasmid#64953Purposemini-Tn7 base vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pF709-pET28a-Hs-FL-ETFbeta-NHis wt
Plasmid#85110Purposeexpression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tagDepositorInserthuman full-length ETFbeta (ETFB Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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TBXTA-c028
Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRK1
Plasmid#39067PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertVRK1 (VRK1 Human)
UseTagsHis TevExpressionBacterialMutationSurface mutant for crystallisation K34A, K35A, E3…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC2735 (pSpy0C2)
Plasmid#220279Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombinationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-tetR_O
Plasmid#127021PurposeBasic Part O- ORF; TetR; transcription factorDepositorInsertPart TetR_o
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK047
Plasmid#65154PurposeEncodes GFP dropout as a Type 234r part to be used in the Dueber YTK systemDepositorInsertGFP dropout
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pTD-C_eYFPTwinStrep
Plasmid#45942Purposeexpresses E.coli codon optimized EYFP-Twin-Strep fusion proteinDepositorInsertsynthetic eYFP (codon usage adapted to E.coli K12)
UseTagsTwin-Strep-tagExpressionBacterialMutationPromoterlacIq/PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
UseTagsGSGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcpP pet29a C-terminal His Tag
Plasmid#75016PurposeExpresses the acyl carrier protein (ACP) from the E. coli fatty acid synthaseDepositorInsertAcpP (acpP E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
G3BP1A
Plasmid#42480PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertG3BP1A
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gi-c010
Plasmid#173080PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGAP
UseTagsmCitrineExpressionBacterialMutationmCitrine + last 10 residues of GNA1, codon optimi…PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VDR_Hormone-recep
Plasmid#109962PurposeProtein expression and purification of VDR_Hormone-recepDepositorInsertVDR_Hormone-recep
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-Cdc42
Plasmid#12201DepositorInsertCdc42 (CDC42 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6f
Plasmid#62811PurposepPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6f-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-hAR-663-919
Plasmid#89083Purposebacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tagDepositorInserthAR ligand binding domain (AR Human)
UseTagsHisExpressionBacterialMutationaa 663-919PromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-3C-irisin
Plasmid#122612PurposeExpresses in E. coli: human irisin with Nt histag and 3C protease site for cleavageDepositorInsertirisin (ectodomain of FNDC5) (Fndc5 Rat, Mouse, Bovine, Human)
UseTagsNt: histag-3C protease siteExpressionBacterialMutationPromoterT7 polymeraseAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP
Plasmid#134357PurposeReporter plasmidDepositorInsertsGFP
mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7Y-SrtA
Plasmid#105601Purposeexpresses 7Y sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7Y mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYDE007
Plasmid#194152PurposesgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.DepositorInsertsgRNA with mrfp control guide sequence
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
miniTn7(Gm)PA1/04/03-ecfp-a
Plasmid#111619PurposeeCFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-ecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF4B
Plasmid#37226DepositorInsertTIF3
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher-mi3
Plasmid#112255PurposeExpresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccinationDepositorInsertSpyCatcher-mi3
UseTagsC-tagExpressionBacterialMutationPromoterT7Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) FLAG pSP65
Plasmid#8537DepositorInsertHOXB7 (HOXB7 Human)
UseIn vitro transcription/translationTagsFLAGExpressionBacterialMutationstarts at AA#1 preceeded by FLAG and Ser-Arg-Ile…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorInsertUbc9 GST fusion (UBE2I Synthetic, Human)
UseTagsGST and GyrA-inteinExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSt-KO
Plasmid#44566Purposefor generation of knockout of mycobacterial genesDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only