32,656 results
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Plasmid#224463PurposeExpression of recombinant Escherichia coli topoisomerase IDepositorInsertEscherichia coli TopA
Tags6xHis tagExpressionBacterialPromoterT7Available SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GFP TEV LIC cloning vector (1GFP)
Plasmid#29663DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags6xHis, GFP, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAR18_StrepI_mFASm_H8_pET22b
Plasmid#122847PurposeExpresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertmouse FASN/ mouse fatty acid synthase (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAF
Plasmid#104152PurposePlasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.DepositorInsertBarrier-to-autointegration factor (BAF) (BANF1 Human)
TagsN-terminal His tag with thrombin cleavage siteExpressionBacterialAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
TagsGSGSGS linker and His6ExpressionBacterialPromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-pBAD
Plasmid#54630PurposeLocalization: Protein Expression Vector , Excitation: 587, Emission: 610DepositorTypeEmpty backboneTags6xHis and mCherryExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PARP1 FL
Plasmid#169815PurposeExpresses codon optimised full length His tagged PARP1 in bacterial cellsDepositorInsertPoly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
TagsMKHHHHHHMKQExpressionBacterialMutationValine 762 mutated to an alaninePromoterT7 promoterAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7Available SinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
ExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-DogCatcher
Plasmid#171929PurposeExpress Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.DepositorInsertCys-DogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7_MBP_TEV_ISCro4_6xHis_T7_term
Plasmid#251502PurposeBacterial expression vector for producing recombinant ISCro4 (E. coli codon optimised)DepositorInsertISCro4 bridge recombinase
ExpressionBacterialMutationNAAvailable SinceFeb. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBB540
Plasmid#27393DepositorInsertgrpE, clpB
ExpressionBacterialAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC18
Plasmid#50004PurposepUC cloning vectorDepositorTypeEmpty backboneExpressionBacterialPromoterlacAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV LIC cloning vector (1S)
Plasmid#29659DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-sfGFP
Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
ExpressionBacterial and PlantAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
EML6428_ pRL662-eGFP
Plasmid#234350PurposeExpressed eGFP(S65T) in agrobacteria for tracing in plantaDepositorInsertSpeR
ExpressionBacterialAvailable SinceNov. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
ExpressionBacterialPromoterownAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSJ55 Accessory plasmid with wt T7 DNAP
Plasmid#253998PurposeAccessory plasmid for LySE, carrying wt T7 DNAP for propagation of phage T7∆DNAPDepositorInsertT7 DNAP (wt)
ExpressionBacterialAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSJ139 Accessory plasmid with T7 DNAP v9
Plasmid#254001PurposeAccessory plasmid for LySE, carrying error-prone T7 DNAP-TadDE (v9) for optimised mutational spectrum.DepositorInsertT7 DNAP v9
ExpressionBacterialAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJOE7706.1
Plasmid#135075PurposeA shuttle vector for heterologous HA production with Corynebacterium glutamicumDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet-I3
Plasmid#189757PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent proteinDepositorInsertmScarlet-I3
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
TagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-Control
Plasmid#122277PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionBacterialPromoterThymidine kinaseAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
TagsNoneExpressionBacterialMutationNonePromoterheat-shock inducibleAvailable SinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-uvsY
Plasmid#163914PurposeExpresses uvsY for bacterial expression and affinity purificationDepositorAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
ExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCherry LIC cloning vector (MBP-mCherry)
Plasmid#29747DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBB542
Plasmid#27395DepositorInsertdnaK, dnaJ, groESL
ExpressionBacterialAvailable SinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP
Plasmid#198753PurposeCre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activityDepositorInsertEF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
UseLentiviralExpressionBacterial and MammalianMutationSee depositor comments belowPromoterEF1aAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_299
Plasmid#250497PurposepTHT15 Origin | Erythromycin Resistance Cassette | Conjugative VectorDepositorInsertermC
UseSynthetic BiologyExpressionBacterialPromoterpErmAvailable SinceMarch 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBXNPH3
Plasmid#110098PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage siteDepositorTypeEmpty backboneTagsHisExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-uvsX-H6
Plasmid#163913PurposeExpresses uvsX for bacterial expression and affinity purificationDepositorInsertuvsX
TagsHisExpressionBacterialPromoterT7 promoterAvailable SinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-parkin
Plasmid#227367PurposeFor bacterial expression of parkinDepositorInsertParkin (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression.Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_25bpLinker
Plasmid#157788PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_25bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-hUBA1
Plasmid#63571PurposeExpression of human UBA1 tagged with HisDepositorAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAKTaq
Plasmid#25712PurposeBacterial expression of Taq polymeraseDepositorInsertTaq polymerase
ExpressionBacterialAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSET Nucleolin
Plasmid#13037DepositorAvailable SinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
ExpressionBacterialPromoterT7Available SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LIC
Plasmid#26094PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis and Thrombin cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
TagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterialAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKEW321-MbCas12a-RFP-GFP-array
Plasmid#115661PurposeEncodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1DepositorInsertGFP and RFP array for MbCas12
UseCRISPRExpressionBacterialPromoterPlacAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV/SB11
Plasmid#26552DepositorInsertSleeping Beauty
ExpressionBacterialMutationMethionine 243 to Glutamine Threonine 136 to Arg…Available SinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFDH
Plasmid#131706PurposeExpress constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coliDepositorInsertformate dehydrogenase
Tagshis-tagExpressionBacterialAvailable SinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIP403
Plasmid#122028PurposePlasmid for expression of gene of interest in Lactobacillus plantarum and L. sakeiDepositorInsertgusA
ExpressionBacterialPromotersppAAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX_hDDX5_FL_wt
Plasmid#88871Purposeinducible expression of GST-DDX5 in bacteriaDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only