Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,669 results

Showing: 7751 - 7800 of 32669 results

  3. pBAD-tdTEVDB_C-15A/G4C

    Plasmid
    #177205
    Purpose
    Permuted td intron with evolved initiation sequence for the expression of circular mRNA
    Depositor
    Stephen Fried
    Article
    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175
    Insert
    Permuted GFP with evolved initiation sequence for circular mRNA
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Permuted sfGFP after residue 52 & Changed Cyt…
    Promoter
    araBAD
    Available Since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177205-plasmid-map-sequence-id-373215

    Enlarge

  4. pGBW-m4046959

    Plasmid
    #145722
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145722-plasmid-map-sequence-id-292485

    Enlarge

  24. 6CC8 (MBD3)

    Plasmid
    #119959
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD3 (MBD3 Human)
    Tags
    GSTtag, 6xHis and Thrombin
    Expression
    Bacterial
    Mutation
    MBD domain (contains residues 1-71)
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119959-plasmid-map-sequence-id-232658

    Enlarge

  30. SUMO-KTag

    Plasmid
    #51723
    Purpose
    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SUMO-KTag
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pETM33_Nsp9

    Plasmid
    #156478
    Purpose
    Bacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156478-plasmid-map-sequence-id-307616

    Enlarge

  46. pET28GSTlinker-LIC

    Plasmid
    #119970
    Purpose
    Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Jan. 9, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119970-plasmid-map-sequence-id-232695

    Enlarge

  48. cr3Dd

    Plasmid
    #184862
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184862-plasmid-map-sequence-id-366226

    Enlarge

Showing: 7751 - 7800 of 32669 results