32,656 results
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Plasmid#13747DepositorAvailable SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only
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pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
ExpressionBacterialPromoterpLacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSJX024
Plasmid#232327PurposeGenome editing in Caulobacter crescentusDepositorInsertssgRNA
SpCas9M
UseCRISPRTagsH-arms and catExpressionBacterialPromoterPJ23119 and PxylAvailable SinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
5KKP (PUS7)
Plasmid#101878PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYHY3
Plasmid#216109PurposeaTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.DepositorInsertNanoLuc
UseSynthetic Biology; E. coli-bacteroides shuttle ve…Tags3xFLAG tag and BT3630 signal peptideExpressionBacterialPromoterP1TDP-A21Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-HNRNPA1LCD
Plasmid#204405PurposeE. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAD-mKate(KPS12)
Plasmid#133831PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPuppAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H4
Plasmid#42633DepositorAvailable SinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXTL-T7max-NanoLuc
Plasmid#188313PurposeExpression of NanoLuc via a T7 promoter in TXTLDepositorInsertNanoLuc
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
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PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorTagsHis6ExpressionBacterialPromoterT7Available SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRM006
Plasmid#98922PurposeExpress Histag-SUMO-FtsZ for purification in E coliDepositorInsertFtsZ
TagsHistag-SUMOExpressionBacterialPromoterT7Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
circRAJ31
Plasmid#69926PurposeExpresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.DepositorInsertplTet-circRAJ31
UseSynthetic BiologyExpressionBacterialPromoterPltetO1-2Available SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLK06Hpp-Fab CR3009
Plasmid#239861PurposePlasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase.DepositorInsertsTagsHexahistidine tag and bacterial alkaline phosphat…ExpressionBacterialMutationF64L, S65T, Q80R, M153T and V163APromoterLac promoterAvailable SinceSept. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDDYFP-A
Plasmid#40288DepositorInsertddYFP-A
TagsHisExpressionBacterialAvailable SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET29b_mNeonGreen
Plasmid#209425PurposeExpress mNeonGreen in E. coliDepositorInsertmNeonGreen
Tags6xHisExpressionBacterialAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH26_pBad_AraC_Para-sfGFP.1TAG
Plasmid#163721PurposeArabinose-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertaraC gfp.1TAG
ExpressionBacterialMutationgfp.N39TAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS305:Met3p-mCherry-Tub1
Plasmid#37553DepositorInsertMet3p-mCherry-Tub1
ExpressionBacterialAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET His6 LIC cloning vector (2Bc-T)
Plasmid#37236DepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS306:Met3p-mCherry-Tub1
Plasmid#37554DepositorInsertMet3p-mCherry-Tub1
ExpressionBacterialAvailable SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rheb(R15C) CL
Plasmid#224266PurposeProtein purification of Rheb (R15C)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D
Plasmid#159569PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12DDepositorInsertHis6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D (KRAS Human)
TagsHis6-MBP-tev-AviExpressionBacterialMutationG12DAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-CTwinStrep
Plasmid#45941Purposeuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTagsPelB and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJUMP44-2A(sfGFP)
Plasmid#126988PurposeLevel 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1mSMAD
Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterGPDAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC572
Plasmid#127973PurposeAMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertpyrG
UseFungal expressionExpressionBacterialPromoternative promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTcI39-PRM-wasabi
Plasmid#195555PurposeTemperature-dependent expression of mWasabi reporter with transcription activation by TcI39DepositorInsertsmWasabi
TcI39
Tags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67TAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
hOtub1
Plasmid#26959DepositorAvailable SinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
p3034 GST-HPV-11 E6
Plasmid#10848DepositorInsertHPV11 E6
TagsGSTExpressionBacterialAvailable SinceOct. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
mCRISPRed pRSETB
Plasmid#159454PurposeExpresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRedDepositorInsertmCRISPRed
Tags10 x His-TagExpressionBacterialPromoterT7Available SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pES002 MBP-GSK3β_S9A-HA-His
Plasmid#196184PurposeExpresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
TagsHA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEcoFus
Plasmid#187833Purpose1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR11 CA NFAT1
Plasmid#11792DepositorInsertNFAT1 (Nfatc2 Mouse)
TagsHAExpressionBacterialMutationConstitutively active: multiple serine to alanine…Available SinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-STAT1
Plasmid#82722PurposeExpresses human STAT1DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-EXO1b D173A
Plasmid#68269PurposeBacterial expression of human EXO1 D173A (catalytically-dead) mutantDepositorInsertEXO1 (EXO1 Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationcatalytically-dead D173A. Please see depositor co…PromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Luciferase
Plasmid#213979PurposeExpresses Doxy Inducible LuciferaseDepositorInsertLuciferase
UseLentiviralTags6xHis affinity tagExpressionBacterial and MammalianPromoterTet-responsive promoter PTight, consisting of sev…Available SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastPromoternoAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM2
Plasmid#32882DepositorInsertMATR3 Matrin3 (MATR3 Human)
TagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 497-566PromoterCMVAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-UBE3C
Plasmid#66711PurposeExpresses UBE3C HECT domain in E. coliDepositorAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG010 (DRT type 2)
Plasmid#157888PurposeExpresses the DRT type 2 defense systemDepositorInsertDRT type 2
ExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-SNAPf
Plasmid#188941PurposeT7 expression vector for making his6- and HA3-tagged SNAP proteinDepositorInserthis6-HA3-SNAPf
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only