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Bacterial Expression

29,729 results

Showing: 4901 - 4950 of 29729 results

  1. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

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  3. 5VC9 (CXXC4)

    Plasmid
    #101263
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC4 (CXXC4 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101263-plasmid-map-sequence-id-197652

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  4. pBS mouse ER81 [TJ#197]

    Plasmid
    #16282
    Depositor
    Thomas Jessell
    Insert
    ER81 (Etv1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 4, 2009
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3.1.Gα12-LgB115

    Plasmid
    #134363
    Purpose
    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα12-LgB115 (GNA12 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134363-plasmid-map-sequence-id-306384

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  16. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid
    #214230
    Purpose
    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75
    Insert
    cSrc kinase SH2 domain (SRC Human)
    Use
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214230-plasmid-map-sequence-id-429073

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  17. pBP-P_cymR-PCymRC

    Plasmid
    #204071
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    cymR repressor and PCymRC cumic acid-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204071-plasmid-map-sequence-id-413887

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  18. LHP2691 - pET30-Ub-K63A

    Plasmid
    #32179
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K63A
    Promoter
    T7
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. LHP2690 - pET30-Ub-K48A

    Plasmid
    #32178
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K48A
    Promoter
    T7
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. p5059

    Plasmid
    #198154
    Purpose
    bacterial expression of steC::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsteC steC::L16::HaloTag::HA
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    198154-plasmid-map-sequence-id-386983

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  24. pSLC-254

    Plasmid
    #124291
    Purpose
    Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-0
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124291-plasmid-map-sequence-id-243082

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  30. pCPP6450

    Plasmid
    #128731
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone
    Depositor
    Alan Collmer
    Article
    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752
    Insert
    HopAD1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    128731-plasmid-map-sequence-id-252571

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  31. p405TDH3

    Plasmid
    #15969
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    p405TDH3 (TDH3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15969-plasmid-map-sequence-id-8454

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  34. pXW117As(RS-E11-RinA)2

    Plasmid
    #123146
    Purpose
    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J117, ParsR, PhrpLE, Pecf11
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123146-plasmid-map-sequence-id-243978

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  36. P48

    Plasmid
    #212155
    Purpose
    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212155-plasmid-map-sequence-id-420758

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  37. pHCL149

    Plasmid
    #134457
    Purpose
    CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    Para-popZ-rbs-H3H4-msfGFPN-CTM.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Para
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134457-plasmid-map-sequence-id-270548

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  40. SPLICSFAST-PO-MT-P2A

    Plasmid
    #214899
    Purpose
    Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-PO-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214899-plasmid-map-sequence-id-423486

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  45. GMFG

    Plasmid
    #39037
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GMFG (GMFG Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 4901 - 4950 of 29729 results