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80,589 results

Showing: 8961 - 9000 of 80589 results

  9. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  17. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177939-plasmid-map-sequence-id-348111

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  19. Tau (P301L)-VC

    Plasmid
    #87633
    Purpose
    expresses hTau fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    MAPT (MAPT Human)
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Mutation P301L
    Promoter
    CMV
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    87633-plasmid-map-sequence-id-196952

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  25. mCherry-Calreticulin-N-16

    Plasmid
    #55006
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55006-plasmid-map-sequence-id-470324

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  28. p-dCas9-JMJD2A-Hygro

    Plasmid
    #104410
    Purpose
    transient expression of dCas9-JMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    JMJD2A (KDM4A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104410-plasmid-map-sequence-id-204464

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  30. GRM1-DuET

    Plasmid
    #213300
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM1 (GRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213300-plasmid-map-sequence-id-430906

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  31. PM-FRB-CFP

    Plasmid
    #67517
    Purpose
    FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)
    Depositor
    Tamas Balla
    Article
    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.
    Insert
    mTOR (MTOR Human)
    Tags
    CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…
    Expression
    Mammalian
    Mutation
    FRB domain only (residues 2019–2114)
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. TFORF2069

    Plasmid
    #141902
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141902-plasmid-map-sequence-id-389798

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  34. ADORA2A-Tango

    Plasmid
    #66210
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADORA2A (ADORA2A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46826-plasmid-map-sequence-id-246440

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  39. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid
    #140984
    Purpose
    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha15-RLuc8 (GNA15 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140984-plasmid-map-sequence-id-300775

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Showing: 8961 - 9000 of 80589 results