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173,143 results

Showing: 5001 - 5050 of 173143 results

  7. mCherry-Rab11a-7

    Plasmid
    #55124
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab11a (RAB11A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55124-plasmid-map-sequence-id-127231

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  9. pLV-TET2BlastR-Cas9-TagBFP

    Plasmid
    #236726
    Purpose
    Dox Inducible Cas-TagBFP expression
    Depositor
    David Bersten
    Insert
    Cas9-TagBFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    236726-plasmid-map-sequence-id-474177

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  12. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Johan Paulsson
    Article
    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    127855-plasmid-map-sequence-id-253039

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  15. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid
    #105540
    Purpose
    AAV expression of EGFP-Cre from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP-Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105540-plasmid-map-sequence-id-202434

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  23. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
    Tags
    NanoLuc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164784-plasmid-map-sequence-id-326673

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  25. RNA-Protein Landing Pad

    Plasmid
    #226162
    Purpose
    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
    Depositor
    Dustin Maly
    Article
    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.
    Inserts
    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    226162-plasmid-map-sequence-id-450826

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  26. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  38. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

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  39. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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Showing: 5001 - 5050 of 173143 results