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177,160 results

Showing: 5001 - 5050 of 177160 results

  1. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid
    #105539
    Purpose
    AAV expression of EGFP from hSyn promoter
    Depositor
    James M. Wilson
    Has service
    AAV1
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40 Articles
    105539-plasmid-map-sequence-id-457689

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  2. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104567-plasmid-map-sequence-id-201429

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  3. pCMV-pro-Il1b-C-Flag

    Plasmid
    #75131
    Purpose
    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    interleukin 1 beta (Il1b Mouse)
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    (please see depositor comments below)
    Promoter
    CMV
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    75131-plasmid-map-sequence-id-192693

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  4. pCI-neo Fluc EGFP

    Plasmid
    #90170
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    90170-plasmid-map-sequence-id-226521

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  5. pCI-neo FlucDM EGFP

    Plasmid
    #90172
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Mutation
    R188Q, R261Q in FLuc
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    90172-plasmid-map-sequence-id-226525

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  7. N174-MCS (Puro)

    Plasmid
    #81068
    Purpose
    Lentiviral vector for constitutive gene expression.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1α
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    81068-plasmid-map-sequence-id-155844

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  11. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Publication
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubiquitin
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    7 Articles
    74939-plasmid-map-sequence-id-151347

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  17. pCDH-PGK

    Plasmid
    #72268
    Purpose
    Express gene of interest under PGK (phosphoglycerate kinase I) promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    7 Articles
    72268-plasmid-map-sequence-id-134573

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  20. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58694-plasmid-map-sequence-id-90691

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  21. GFP-PBRM1

    Plasmid
    #65387
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PBRM1 (PBRM1 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65387-plasmid-map-sequence-id-322048

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  22. GFP-BRD3

    Plasmid
    #65377
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRD3 (BRD3 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    65377-plasmid-map-sequence-id-287374

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  27. mCherry-Rab7a-7

    Plasmid
    #55127
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab7a (RAB7A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    55127-plasmid-map-sequence-id-311825

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  28. mCherry-Beta-Catenin-20

    Plasmid
    #55001
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    55001-plasmid-map-sequence-id-300582

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  31. pCS-H2B-mRFP1

    Plasmid
    #53745
    Purpose
    Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector
    Depositor
    Sean Megason
    Publication
    Megason et al Methods Mol Biol. 2009;546:317-32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    histone H2B (H2BC21 Human)
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    53745-plasmid-map-sequence-id-242469

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  32. mKeima-Red-C1

    Plasmid
    #54546
    Purpose
    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKeima-Red
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    54546-plasmid-map-sequence-id-256470

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  36. pcDNA3.1-MAP3K5 (WT)

    Plasmid
    #47104
    Purpose
    Expresses wild type human MAP3K5 in mammalian cells
    Depositor
    Yardena Samuels
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP3K5 (MAP3K5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    CMV
    Available since
    Jan. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    47104-plasmid-map-sequence-id-214692

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Showing: 5001 - 5050 of 177160 results