8,689 results

1. pcDNA3 HA Arf1 DN T31N

   Plasmid

   #10833

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf1 T31N (ARF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T31N. Defective in GTP binding. Dominant negati…

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFR_CrPV_xb

   Plasmid

   #11509

   Depositor

   Phil Sharp

   Article

   Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

   Insert

   Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEG BacMam C term 3C eGFP StrepII

   Plasmid

   #160686

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   3C eGFP StrepII

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Wnt3A

   Plasmid

   #35908

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt3A (WNT3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. N174-MCS

   Plasmid

   #81061

   Purpose

   Lentiviral vector for constitutive gene expression.

   Depositor

   Adam Karpf

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hSOX2

   Plasmid

   #17218

   Purpose

   Retroviral expression of human SOX2

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBABE-zeo largeTgenomic

   Plasmid

   #1778

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   large T and small T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-FGFR1

   Plasmid

   #116740

   Purpose

   Lentiviral expression of FGFR1

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FGFR1 (FGFR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1_MeCP2(WT)

   Plasmid

   #110186

   Purpose

   Expression of mouse MeCP2 (WT) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223-FLT1

    Plasmid

    #23912

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    S755P

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-TREX1

    Plasmid

    #27218

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ142

    Plasmid

    #69294

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC034 - LwCas13a-msfGFP-2A-Blast

    Plasmid

    #91924

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-TPX2

    Plasmid

    #31227

    Depositor

    Patricia Wadsworth

    Article

    Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

    Insert

    TPX2 (TPX2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV R-CEPIA3mt

    Plasmid

    #140464

    Purpose

    Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

    Insert

    R-CEPIA3mt
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-SADB19N

    Plasmid

    #32630

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    B19N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP_TRPM8

    Plasmid

    #64879

    Purpose

    Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

    Insert

    Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-sh1368 knockdown c-myc

    Plasmid

    #29435

    Depositor

    Bob Eisenman

    Article

    Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

    Insert

    c-Myc shRNA (MYC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP and WPRE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMGF182

    Plasmid

    #97006

    Purpose

    Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    CHMP7 (CHMP7 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV delta 5

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-HA PTEN

    Plasmid

    #78776

    Purpose

    To overexpress PTEN in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBPGAL4.2::VP16Uw

    Plasmid

    #26228

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL4::VP16 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEFmEGFP-mDia2

    Plasmid

    #25407

    Depositor

    Arthur Alberts

    Article

    Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

    Insert

    mDia2 (Diaph3 Mouse)
    

    Use

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Encodes mDia2

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid

    #177672

    Purpose

    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

    Depositor

    Karl Deisseroth

    Has Service

    AAV8

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCW57.1-DUX4-CA

    Plasmid

    #99281

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (codon altered) (DUX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-tetO-myc T58A

    Plasmid

    #19763

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

    Plasmid

    #31476

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14 GFP donor EF1s I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    GFP has 14 amino acids deleted from c terminus

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-SADB19G

    Plasmid

    #32633

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CoV2-N-R203M

    Plasmid

    #177952

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203M) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203M

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Str-Ii_VSVG-SBP-mCherry

    Plasmid

    #65301

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p3XFLAG-CMV-14-WDR5

    Plasmid

    #59974

    Purpose

    Mammalian Expression of WDR5-FLAG

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-Tag2B EDD

    Plasmid

    #37188

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Use

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    CMV

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMVTNT PINK1 N-myc

    Plasmid

    #13313

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pC1-HyPer-Red-C199S

    Plasmid

    #48252

    Purpose

    Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED-C199S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLV-tTRKRAB-red

    Plasmid

    #12250

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Insert

    TetR-KRAB fusion and dsRED
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    dsRed added to backbone.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GST-RBD

    Plasmid

    #15247

    Depositor

    Martin Schwartz

    Article

    Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

    Insert

    Rhotekin (Rtkn Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 7-89 (Rho binding domain)

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET302-6His-dCas9-Halo

    Plasmid

    #72269

    Purpose

    Encodes a Halo-labeled fusion of nuclease deficient Cas9

    Depositor

    Timothée Lionnet

    Article

    Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

    Insert

    His6-dCas9-Halo
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBUE411

    Plasmid

    #62200

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    OsU3 and Ubi

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TOPO mKate2

    Plasmid

    #68441

    Purpose

    TOPO construct expressing mKate2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mKate2
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge