8,883 results

Showing: 4041 - 4080 of 8883 results

Results per page:

Sort results by:

pDARMO_HUWE1_3xFLAG

Plasmid

#187155

Purpose

mammalian cell expression of HUWE1-3xFLAG

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 BRGI-Flag

Plasmid

#19143

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-14-3-3 beta

Plasmid

#13270

Depositor

Michael Yaffe

Insert

14-3-3 beta (YWHAB Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-VSVG

Plasmid

#1733

Purpose

VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab

Depositor

Garry Nolan

Insert

VSV-G Envelope

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
OmpA3-PNGaseF-TEV-His6

Plasmid

#114274

Purpose

Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

Depositor

Martin Caffrey

Insert

Peptide: N-glycosidase F

Tags

OmpA3 leader sequence (for periplasmic localisati…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST14-SpyCatcher

Plasmid

#35044

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-hOCT3/4

Plasmid

#17217

Purpose

Retroviral expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

OCT3/4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
FBP17-pmCherryC1

Plasmid

#27688

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FBP17 (FNBP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pLenti CMV/TO Zeo DEST (644-1)

Plasmid

#17294

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHsp70-Cas9

Plasmid

#45945

Purpose

The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

3x FLAG

Mutation

Silent change from C to A at bp 834 of insert

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
M57 pPET28a-TEV-full-length human beta catenin

Plasmid

#17198

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Tags

HIS

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-P4M-SidM

Plasmid

#51471

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Insert

P4M-SidM(546-647)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-CRBN-pGK-HYG

Plasmid

#107380

Purpose

Mammalian expression of FLAG-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MST1

Plasmid

#1965

Depositor

Joseph Avruch

Article

Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

Insert

MST1 (Stk4 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

Plasmid

#31484

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

EF1s HA NLS Sce(opt) T2A IFP1.4

Use

Lentiviral

Tags

HA and NLS

Mutation

"Opt" indicates the I-SceI coding seque…

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET His6 GFP TEV LIC cloning vector (1GFP)

Plasmid

#29663

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, GFP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBFP2-C1

Plasmid

#22880

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

Insert

SBFP2

Expression

Mammalian

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-X1-Neo-GFP-FAM134B

Plasmid

#109026

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-Ubiquitin-K29

Plasmid

#22903

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-HSF1

Plasmid

#32537

Depositor

Stuart Calderwood

Article

Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

Insert

HSF1 (HSF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

see depositor comments below

Promoter

CMV

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET His6 MBP N10 TEV LIC cloning vector (2C-T)

Plasmid

#29706

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Article

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiPGK Hygro DEST H2B-mRuby2

Plasmid

#90236

Purpose

Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hAAVS1 1R TALEN

Plasmid

#35432

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1R TALEN

Expression

Mammalian

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
ErkKTR-irFP

Plasmid

#111510

Purpose

Reporter of Erk activity

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

ERK KTR (Mapk1 Mouse)

Use

Lentiviral

Tags

iRFP

Promoter

SSFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-puro-CMV-RC3AI

Plasmid

#78908

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

RC3AI

Use

Lentiviral

Tags

HA

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GAPDH-CPG-TV

Plasmid

#70148

Purpose

a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4041 - 4080 of 8883 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests

More Filters

Applied Filters

Applied Filters

8,883 results