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8,962 results

Showing: 5601 - 5650 of 8962 results

  1. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50454-plasmid-map-sequence-id-214300

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  4. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available Since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

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  17. pZac2.1-GfaABC1D-lck-jGCaMP8s

    Plasmid
    #176761
    Purpose
    AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Has Service
    AAV1 and AAV9
    Insert
    lck-jGCaMP8s
    Use
    AAV
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176761-plasmid-map-sequence-id-349053

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  19. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    Zhe J. Liu
    Article
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91564-plasmid-map-sequence-id-180643

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  20. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid
    #101178
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101178-plasmid-map-sequence-id-219907

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  28. pZ8-Ptac

    Plasmid
    #74064
    Purpose
    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74064-plasmid-map-sequence-id-138074

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  30. p415-GalL-Cas9-CYC1t

    Plasmid
    #43804
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GalL
    Available Since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43804-plasmid-map-sequence-id-59154

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  31. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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  35. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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  36. mEmerald-CD9-10

    Plasmid
    #54029
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CD9 (CD9 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54029-plasmid-map-sequence-id-266657

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  39. PBX1A-pCMV1

    Plasmid
    #21029
    Depositor
    Corey Largman
    Insert
    PBX1 (PBX1 Human)
    Expression
    Mammalian
    Available Since
    Aug. 14, 2009
    Availability
    Academic Institutions and Nonprofits only

  43. pTwist-SARS-CoV-2 Δ18 B.1.1.529

    Plasmid
    #179907
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179907-plasmid-map-sequence-id-351282

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  47. pYLCRISPR/Cas9P35S-N

    Plasmid
    #66191
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66191-plasmid-map-sequence-id-404047

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  49. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid
    #141183
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141183-plasmid-map-sequence-id-288058

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Showing: 5601 - 5650 of 8962 results