9,047 results

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pFRT/TO/HIS/FLAG/HA-PRDX1

Plasmid

#38086

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PRDX1 peroxiredoxin 1 (PRDX1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

Plasmid

#130998

Purpose

Double floxed ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

Ef1a

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Promoter

EF1-Alpha

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-Nanog

Plasmid

#16351

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Expression

Bacterial

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-BbTagBY

Plasmid

#45185

Depositor

Dawen Cai, Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Has Service

AAV9

Insert

pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE

Use

AAV; Adeno-associated viral

Expression

Mammalian

Promoter

human EF1a

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight Neo DEST (w770-1)

Plasmid

#26432

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Expression

Mammalian

Available Since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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EYFP-hCaM

Plasmid

#47603

Depositor

Emanuel Strehler

Article

Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

Insert

CaM (CALM3 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pB1H1

Plasmid

#12611

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Dorsal RHR in pB1H1 (dl Fly)

Tags

flag

Expression

Bacterial

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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mCherry-Tubulin-C-18

Plasmid

#55148

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcdna3 flag - ULK1 wt

Plasmid

#27636

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK1 (Ulk1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M from ULK1 removed.

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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PGK1p-Csy4-pA (Construct 2)

Plasmid

#55196

Purpose

Expresses Csy4 in Mammalian cells for site-specific RNA processing

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

Csy4/Cas6

Use

Synthetic Biology

Tags

6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-MERTK

Plasmid

#23900

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MERTK (MERTK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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GST-14-3-3wt (pEBG)

Plasmid

#1942

Depositor

Joseph Avruch

Article

Tzivion et al Nature 1998 Jul 2;394(6688):88-92.

Insert

14-3-3 zeta (Ywhaz Mouse)

Tags

GST

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA1

Plasmid

#26677

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 1 (KPNA1 Human)

Tags

T7

Expression

Mammalian

Mutation

KPNA1 sequence contains a reported SNP at S73N

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-tagged FRB (YR)

Plasmid

#20148

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FRB (YR)

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mNaChBac-T2A-tdTomato

Plasmid

#60650

Purpose

Expresses mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBac-T2A-tdTomato

Expression

Mammalian

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Article

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Article

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFR-PATagRFP

Plasmid

#31950

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

EGF receptor (EGFR)

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-neo-CD2-FAK

Plasmid

#37013

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

CD2-FAK (CD2, PTK2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Talin1

Plasmid

#26724

Depositor

Anna Huttenlocher

Article

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Insert

Talin (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pPET-PKR/PPase

Plasmid

#42934

Depositor

James Cole

Article

Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

Inserts

protein kinase R (EIF2AK2 Human)
phage lambda phosphatase

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEP4 E02S EM2K

Plasmid

#20923

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6/V5-p53_R249S

Plasmid

#22935

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R249S Mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-N-R203M

Plasmid

#177952

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203M) (N )

Expression

Mammalian

Mutation

R203M

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SENP1 (catalytic domain)

Plasmid

#16356

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP1 catalytic domain (SENP1 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 419 to 644 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-FilaminA-N-9

Plasmid

#54098

Purpose

Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

FilaminA (FLNA Human)

Tags

mEmerald

Expression

Mammalian

Mutation

D2626H

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-Casp3 C163A-myc

Plasmid

#11814

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

Myc

Expression

Mammalian

Mutation

C163A

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Article

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
human MMP-2 promoter

Plasmid

#53432

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

Insert

MMP2 promoter (MMP2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

MMP2

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Article

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-SFPQ

Plasmid

#166959

Purpose

Expresses HA-tagged SFPQ protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CBFRE-EGFP

Plasmid

#17705

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

CBFRE

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLionII

Plasmid

#1730

Purpose

This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system

Depositor

Garry Nolan

Type

Empty backbone

Use

Lentiviral

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FlagMLL-pl-ENL(5613)

Plasmid

#20873

Depositor

Robert Slany

Article

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL (KMT2A, MLLT1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
138-dCas9-Dnmt3a

Plasmid

#84570

Purpose

PiggyBac transposon system construct with dCas9-Dnmt3a

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Expression

Mammalian

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFR_CrPV_xb

Plasmid

#11509

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Available Since

March 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
YPET-GGA3

Plasmid

#18841

Depositor

Martin Schwartz

Article

Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Insert

YPET-GGA3 (GGA3 Human)

Tags

His6 and myc

Expression

Mammalian

Mutation

The fluorescent protein YPET was added to the ami…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only
NPC1 His6 EGFP

Plasmid

#53521

Depositor

Matthew Scott

Article

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Tags

EGFP and His6

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6b:sgRNA#3

Plasmid

#64247

Purpose

expresses sgRNA under U6b promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pREP-NFAT4

Plasmid

#11790

Depositor

Anjana Rao

Insert

NFAT4 (NFATC3 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-TPX2

Plasmid

#31227

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1 EGFP-Tau

Plasmid

#108868

Purpose

Expresses EGFP linked to human Tau

Depositor

Gabriele Kaminski Schierle

Insert

Tau (MAPT Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

human four-repeat tau lacking the N-terminal sequ…

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MRLC1

Plasmid

#35680

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-FlpeERT2

Plasmid

#14756

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe-ERT2 fusion protein

Use

Flp/frt

Tags

ERT2

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge

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9,047 results