Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,777 results

Showing: 5901 - 5950 of 163777 results

  11. pLEXSY-hyg2.1_LtIFT43_Flag

    Plasmid
    #194433
    Purpose
    Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae
    Depositor
    Alan Brown
    Article
    Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.
    Insert
    Intraflagellar transport complex subunit 43
    Use
    Leishmania tarentolae expression
    Tags
    3X Flag and Linker
    Expression
    Mutation
    Promoter
    L.donovani promoter elements
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    194433-plasmid-map-sequence-id-383732

    Enlarge

  12. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid
    #99248
    Purpose
    single-vector system with EGFP reporter for use in CROPseq knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99248-plasmid-map-sequence-id-191832

    Enlarge

  17. pPBbsr2-AREG-ScNeo

    Plasmid
    #209910
    Purpose
    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    AREG-ScNeo (AREG Human)
    Use
    Tags
    mNeonGreen and mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209910-plasmid-map-sequence-id-416693

    Enlarge

  20. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

    Enlarge

  21. PB-SAM-Dest

    Plasmid
    #102563
    Purpose
    Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    N/A
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102563-plasmid-map-sequence-id-193893

    Enlarge

  27. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

    Enlarge

  30. pDEST-CMV mCherry-GFP-LC3B WT

    Plasmid
    #123230
    Purpose
    Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123230-plasmid-map-sequence-id-244035

    Enlarge

  36. pBluescript-minimal PB

    Plasmid
    #177151
    Purpose
    Minimal piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177151-plasmid-map-sequence-id-348859

    Enlarge

  37. mEmerald-Vimentin-N-18

    Plasmid
    #54301
    Purpose
    Localization: Intermediate Filaments, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. SPLICSFAST-LDh-MT-P2A

    Plasmid
    #214896
    Purpose
    Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214896-plasmid-map-sequence-id-423482

    Enlarge

  43. psPAX2-D64V-NC-MS2

    Plasmid
    #122944
    Purpose
    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
    Depositor
    Baisong Lu
    Article
    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531
    Insert
    NC-MCP (MS2 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D64V
    Promoter
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122944-plasmid-map-sequence-id-239322

    Enlarge

Showing: 5901 - 5950 of 163777 results