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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,258 results

Showing: 5901 - 5950 of 159258 results

  1. TFORF0282

    Plasmid
    #141561
    Purpose
    Lentiviral vector for overexpressing the MEF2C transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MEF2C (MEF2C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141561-plasmid-map-sequence-id-276819

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  3. pAK_DR30_EF1a_CasRx_Puro

    Plasmid
    #134842
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    134842-plasmid-map-sequence-id-266572

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  20. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62858-plasmid-map-sequence-id-243538

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  21. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Use
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  25. PB-TAG-ERN

    Plasmid
    #80476
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80476-plasmid-map-sequence-id-153664

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  30. pRS410

    Plasmid
    #11258
    Depositor
    Fred Cross
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11258-plasmid-map-sequence-id-6932

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  37. Sniper2L

    Plasmid
    #193856
    Purpose
    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193856-plasmid-map-sequence-id-382455

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  40. ADA

    Plasmid
    #39020
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADA (ADA Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  42. TFORF0074

    Plasmid
    #142144
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BACH2 (BACH2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142144-plasmid-map-sequence-id-397722

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  43. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    51295-plasmid-map-sequence-id-339890

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  45. CMV-R-GECO1

    Plasmid
    #32444
    Purpose
    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    R-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32444-plasmid-map-sequence-id-117093

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  50. pAAV-hsyn-NE2m

    Plasmid
    #208686
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons
    Depositor
    Yulong Li
    Insert
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208686-plasmid-map-sequence-id-412172

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Showing: 5901 - 5950 of 159258 results