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161,805 results

Showing: 5901 - 5950 of 161805 results
  1. TFORF1317

    Plasmid
    #143788
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    RB1CC1 (RB1CC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. MRGPRD-DuET

    Plasmid
    #213340
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    MRGPRD (MRGPRD Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Insert
    Cereblon (CRBN Human)
    Use
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. Twin-Strep(R)-Tev-GSGS-Ubiquitin

    Plasmid
    #186803
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.
    Depositor
    Insert
    Twin-Strep(R)-Tev-GSGS-Ubiquitin
    Use
    Tags
    GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…
    Expression
    Bacterial
    Mutation
    Strep tag Lysine's mutated to Arginine
    Promoter
    Available since
    March 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    IL-2 promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
  7. EF1a- gfp-2a-puro

    Plasmid
    #129443
    Purpose
    expression of GFP and Puro R
    Depositor
    Insert
    gfp-puro
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. N1-Mito-pHCountdown

    Plasmid
    #182303
    Purpose
    mammalian cell plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence
    Depositor
    Insert
    mitochondria targeted pH countdown
    Use
    Tags
    COX8A mitochondria targeting sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV-CaMKIIa-EGFP

    Plasmid
    #50469
    Purpose
    EGFP under the control of CaMKIIa promoter
    Depositor
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
  10. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid
    #105550
    Purpose
    AAV expression of Cre from GFAP promoter
    Depositor
    Has Service
    AAV PHP.eB and AAV5
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GFAP
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. LiOn-CMV∞Cre

    Plasmid
    #154018
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposase
    Depositor
    Insert
    Cre-FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 5901 - 5950 of 161805 results