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170,823 results

Showing: 5901 - 5950 of 170823 results

  1. pCAGGS-FLAG-BubR1

    Plasmid
    #200814
    Purpose
    expresses BubR1 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200814-plasmid-map-sequence-id-410103

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  3. pKSEe401R

    Plasmid
    #218532
    Purpose
    CRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.
    Depositor
    Kirill Demchenko
    Article
    Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    218532-plasmid-map-sequence-id-458122

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  4. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  7. pJRA101

    Plasmid
    #209335
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mVenus fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209335-plasmid-map-sequence-id-420456

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  9. CBP core-dCas9-p300 core

    Plasmid
    #179558
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179558-plasmid-map-sequence-id-354773

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  10. GS2.1/EC

    Plasmid
    #132568
    Purpose
    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).
    Depositor
    Mathias Pribil
    Article
    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111
    Insert
    egg cell promoter, Cas9-mApple, pds3 sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    A. thaliana egg cell promoter
    Available Since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    132568-plasmid-map-sequence-id-262634

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  11. FDA60

    Plasmid
    #45606
    Depositor
    David Baker
    Insert
    FDA60
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. LV-tetO-EF1a-GFP

    Plasmid
    #187645
    Purpose
    Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites
    Depositor
    Martin Carroll
    Insert
    GFP
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187645-plasmid-map-sequence-id-367456

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  20. BK beta4 EGFP

    Plasmid
    #113560
    Purpose
    human BK beta4 subunit in C-term translational frame with EGFP (EGFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb4 (KCNMB4 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113560-plasmid-map-sequence-id-218925

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  32. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available Since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

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  36. pRBC-sfGFP-150TAG

    Plasmid
    #174076
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,
    Depositor
    Ryan Mehl
    Article
    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15
    Insert
    Superfolder GFP
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    T7
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    174076-plasmid-map-sequence-id-345697

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  37. pIS1

    Plasmid
    #12179
    Depositor
    David Bartel
    Type
    Empty backbone
    Use
    Luciferase
    Expression
    Mammalian
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12179-plasmid-map-sequence-id-234698

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Showing: 5901 - 5950 of 170823 results