8,789 results

1. pcDNA5/FRT-HA-hM3D(Gq)

   Plasmid

   #45547

   Purpose

   Expresses hM3D (Gq) neuronal activator

   Depositor

   Bryan Roth

   Article

   Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

   Insert

   hM3D (Gq)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Tet3G blasticidin

   Plasmid

   #128061

   Purpose

   Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-dR8.2 dvpr

   Plasmid

   #8455

   Purpose

   2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pComb3XSS

   Plasmid

   #63890

   Purpose

   3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Inserts

   SS Stuffer
   Light Chain Stuffer
   Heavy Chain Stuffer
   

   Use

   Phage display

   Tags

   6x His, HA tag, and geneIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEVOL-pylT-N346A/C348A

   Plasmid

   #127411

   Purpose

   Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

   Depositor

   Wenshe Liu

   Article

   Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N346A-C348A

   Promoter

   araBAD

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLX303-ZIM3-KRAB-dCas9

   Plasmid

   #154472

   Purpose

   Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-HA-Ubiquitin-K63

   Plasmid

   #17606

   Purpose

   Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K63 only, other lysines mutated to arginines. Enh…

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+HyPer7

    Plasmid

    #136466

    Purpose

    Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-HA-Ubiquitin-K48

    Plasmid

    #17605

    Purpose

    Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48 only, other lysines mutated to arginines. Enh…

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVUT-tTR-KRAB

    Plasmid

    #11651

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Has Service

    Cloning Grade DNA

    Insert

    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+MLS-HyPer7

    Plasmid

    #136470

    Purpose

    Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Tandem mitochondrial targeting signal of cytochro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ub-G76V-GFP

    Plasmid

    #11941

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-G76V-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. ER-mNeonGreen

    Plasmid

    #137804

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmCherry-Gal3

    Plasmid

    #85662

    Purpose

    Fluorescent reporter for lysosomal damage

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLJM1-FLAG-GFP-Tmem192

    Plasmid

    #134630

    Purpose

    lysosomal marker

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMIG-FLAG-MLL-AF9

    Plasmid

    #71443

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-Af9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-loxPSTOPloxP-ZsGreen

    Plasmid

    #51269

    Purpose

    Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    loxP-STOP-loxP-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLJM1-EGFP

    Plasmid

    #19319

    Purpose

    3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-EF1

    Plasmid

    #72266

    Purpose

    Express gene of interest under EF-1 promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pAC-BETA

    Plasmid

    #53272

    Purpose

    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

    Insert

    crtE, crtY, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Active Wnt5A-V5

    Plasmid

    #43813

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT5A (WNT5A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSIP403

    Plasmid

    #122028

    Purpose

    Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

    Depositor

    Lars Axelsson, Geir Mathiesen

    Article

    Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

    Insert

    gusA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sppA

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-ERBB2

    Plasmid

    #116734

    Purpose

    Lentiviral expression of ERBB2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE_puro

    Plasmid

    #118692

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Olig001

    Plasmid

    #170716

    Purpose

    Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

    Depositor

    Thomas McCown

    Article

    Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

    Insert

    Olig001 capsid gene / rep 2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSIE1

    Plasmid

    #136355

    Purpose

    Selection by Inducible Effectors

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    P1TDP-GHO23

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    MBP

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-HA-JMJD3

    Plasmid

    #24167

    Purpose

    Mammalian expression of human JMJD3 with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3 HA Arf6 ActQ67L

    Plasmid

    #10835

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 Q67L (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L. Defective in GTP hydrolysis. Constitutive…

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-myc3-HDM2

    Plasmid

    #20935

    Depositor

    Yue Xiong

    Article

    Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

    Insert

    Mdm2 p53 binding protein homolog (MDM2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUCmini-iCAP-PHP.eB

    Plasmid

    #103005

    Purpose

    non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    Synthetic construct isolate AAV-PHP.eB VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pmGFP-P2A-K0-P2A-RFP

    Plasmid

    #105686

    Purpose

    Double fluorescent stalling reporter K0

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K0
    P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge