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19,181 results

Showing: 7201 - 7250 of 19181 results

  1. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Article
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    118632-plasmid-map-sequence-id-230217

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  5. p5'Entry-lck-7.4kb

    Plasmid
    #58891
    Purpose
    provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes
    Depositor
    Jeffrey Yoder
    Insert
    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
    Use
    Gateway 5' entry clone
    Tags
    Expression
    Mutation
    mutated single nucleotide (T-->C, 1874th nucle…
    Promoter
    lymphocyte-specific protein tyrosine kinase (lck)…
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58891-plasmid-map-sequence-id-91398

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  13. pCS2+ H2B-mTagBFP2

    Plasmid
    #99267
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Insert
    mTagBFP2-3XHA
    Use
    Tags
    H2B
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99267-plasmid-map-sequence-id-192954

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  14. GRM2-Tango

    Plasmid
    #66388
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GRM2 (GRM2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66388-plasmid-map-sequence-id-417851

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  16. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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  18. pHCAG-L2EOP

    Plasmid
    #51783
    Purpose
    The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expression
    Depositor
    Paula Lam
    Article
    Sia et al J Gene Med. 2010 Oct;12(10):848-58. doi:
    Inserts
    Luciferase-2A-EBNA-1
    OriP
    Use
    Herpes simplex virus type-1 amplicon vector
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51783-plasmid-map-sequence-id-79421

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  20. pLEX307-TMPRSS2-blast

    Plasmid
    #158458
    Purpose
    Constitutive lentiviral expression of human TMPRSS2.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158458-plasmid-map-sequence-id-316882

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  26. Cilantro 2

    Plasmid
    #74450
    Purpose
    Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74450-plasmid-map-sequence-id-140420

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  31. pORTMAGE-4

    Plasmid
    #72679
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72679-plasmid-map-sequence-id-198908

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  32. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  37. pTara:500

    Plasmid
    #60717
    Purpose
    Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60717-plasmid-map-sequence-id-97574

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  39. pETcon(-)

    Plasmid
    #41522
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Tags
    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    41522-plasmid-map-sequence-id-59598

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  42. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140082-plasmid-map-sequence-id-292704

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  46. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Use
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

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Showing: 7201 - 7250 of 19181 results