8,964 results

Showing: 7201 - 7250 of 8964 results

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS5A

Plasmid

#17646

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS5A (POLY Hepatitis C virus)

Tags

Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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Myc-Foxo1 D256

Plasmid

#12145

Depositor

Domenico Accili

Article

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Insert

Foxo1 (Foxo1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Contains a deletion truncating the protein after …

Promoter

CMV

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9

Plasmid

#28100

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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eTC GFP beta-actin full length

Plasmid

#27123

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

E237A

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.mFoxa2

Plasmid

#33014

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Forkhead box A2 (Foxa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

H10L

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

Plasmid

#85436

Purpose

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab and anti-CTLA4 scFv

Use

Lentiviral

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only
GFP-LKB1

Plasmid

#21147

Depositor

Junying Yuan

Article

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1 (Stk11 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-HA-Sox2-IP

Plasmid

#13459

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Sox2 (Sox2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf2

Plasmid

#13678

Depositor

Ying Zhang

Article

Zhang et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

Smurf2 (SMURF2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-syn-FLEX-splitTVA-EGFP-tTA

Plasmid

#100798

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

TVA, EGFP, tet transactivator

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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V246 pCEP 4HA B56beta

Plasmid

#14533

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5B B56 beta regulatory subunit of PP2A (PPP2R5B Human)

Tags

4 HA

Expression

Mammalian

Mutation

I346V relative to GenBank ID L42374

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGW1-Myc-DJ1-WT

Plasmid

#29347

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV6-p85alpha-Flag

Plasmid

#1399

Depositor

Lewis Cantley

Insert

PI3K regulatory subunit p85alpha (Pik3r1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p4054 HA-E6AP isoform II

Plasmid

#8658

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform II (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

Plasmid

#141394

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
phOCT4-Luc

Plasmid

#17221

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human OCT4 (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

Plasmid

#35509

Purpose

AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV5 and AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
hu-TLR4 cDNA wt pDEST40

Plasmid

#42646

Purpose

destination vector for expression of human TLR4 in mammalian cells

Depositor

Scott Friedman

Article

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 (TLR4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only
hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf7b-IRES-Puro

Plasmid

#141389

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf7b (ORF7b SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRK5-EGFP-Tau AP P301L

Plasmid

#46906

Purpose

expresses EGFP tagged Tau AP P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP P301L (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
N-Myc-53BP1 WT pLPC-Puro

Plasmid

#19836

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A) receptor subunit g2SE

Plasmid

#49170

Purpose

pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

Insert

GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)

Tags

myc, pHGFP (pHluorin), and thrombin cleavage site…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MPZ(P0)promoter pGL3-TATA

Plasmid

#21259

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

myelin protein zero promoter (Mpz Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3 NSE promoter

Plasmid

#11606

Depositor

Rosalyn Adam

Article

Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

Insert

NSE promoter (ENO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Ai34

Plasmid

#34881

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

CAG-Floxed Synaptophysin-tdTomato

Use

Mouse Targeting

Tags

tdTomato

Promoter

CAG

Available Since

March 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pB1H1

Plasmid

#12611

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Dorsal RHR in pB1H1 (dl Fly)

Tags

flag

Expression

Bacterial

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-JAK1

Plasmid

#23932

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

JAK1 (JAK1 Human)

Use

Gateway Cloning

Mutation

R647H (numbering relative to NP_002218.1)

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMX-Gal4-TEAD4

Plasmid

#33105

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD4 (TEAD4 Human)

Tags

Gal4

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-mCh-empty

Plasmid

#21907

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe EGFR(T790M)

Plasmid

#32070

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-HDAC4-FLAG

Plasmid

#30485

Depositor

Tso-Pang Yao

Insert

HDAC4 (HDAC4 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-NL1B

Plasmid

#15261

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert B (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRIME-TET-GFP-FF3

Plasmid

#11662

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR human Drosha1

Plasmid

#14766

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pQCXI Puro DsRed-LC3-GFP

Plasmid

#31182

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
TRMPVIR

Plasmid

#27994

Purpose

Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPVIR (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pmStrawberry-Atg4BC74A

Plasmid

#21076

Depositor

Tamotsu Yoshimori

Article

Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

Insert

Atg4B(C74A) (Atg4b Mouse)

Tags

mStrawberry

Expression

Mammalian

Mutation

220-222 TGC(Cys) is changed to GCA (Ala)

Available Since

June 8, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG LIP

Plasmid

#15737

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG426GAL-ccdB-EGFP

Plasmid

#14203

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV Flag WT-PTEN

Plasmid

#22231

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

Insert

PTEN (PTEN Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-hPIASx alpha

Plasmid

#15209

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx alpha (PIAS2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

Plasmid

#32739

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (Tcf7l2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N-terminal deletion form (delta N) lacks amin…

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-shBRCA1 #1

Plasmid

#44594

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

BRCA1 (Brca1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pLOVE-miR302/367

Plasmid

#31310

Depositor

Ed Morrisey

Article

Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

Insert

mmu-miR302/367

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only
IRES-puro-GFP

Plasmid

#16616

Depositor

Bert Vogelstein

Article

Torrance et al Nat Biotechnol. 2001 Oct . 19(10):940-5.

Insert

GFP

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,964 results