169,365 results
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Plasmid#32674DepositorTypeEmpty backboneUseZebrafish transgenesisTagsEGFPAvailable SinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only
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pMXS-IRES-BLAST EGFP-flag-CAD
Plasmid#188118PurposeExpresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsEGFP tag and Flag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…Available SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11R
Plasmid#121154PurposeMammalian expression of HA tagged ubiquitin K11RDepositorAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTG612
Plasmid#237387PurposemStayGold^NSEC^ repair templateDepositorInsertmStayGold^NSEC^
Available SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTG613
Plasmid#237388PurposemStayGold::AID^NSEC^ repair templateDepositorInsertmStayGold::AID^NSEC^
Available SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAL750 :: msfGFP
Plasmid#250661PurposeInducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax volcaniiDepositorInsertMonomeric Superfolder Green Fluorescent Protein
UseHaloarchaeal expressionAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)
Plasmid#242591PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an H2A-tdiRFP for nuclear labeling.DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTCas9_scr
Plasmid#207566PurposeThermoCas9-mediated cleavage of genomic DNA in E. coliDepositorInsertPlac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICSL12013
Plasmid#245630PurposeLevel 0 Golden Gate part 6GAL4UAS promoter, GGAG - AATGDepositorInsert6GAL4UAS promoter
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI (JDW 1494)
Plasmid#242592PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state.DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mScarlet3-S2 (JDW 1514)
Plasmid#242552PurposeGateway middle entry clone containing H2B-mScarlet3-S2; Nuclear red fluorescent reporterDepositorInsertmScarlet3-S2
UseGateway subcloningAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato
Plasmid#244921PurposeiATPSnFR2.A95K variant with non-responsive tdTomatoDepositorInsertiATPSnFR2.A95K.tdTomato
UseAAVMutationA95KAvailable SinceNov. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-Pho8∆60-HphMX6
Plasmid#240973PurposeTo endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection markerDepositorInsertPHO8∆60 (PHO8 Budding Yeast)
ExpressionBacterialAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-hCA4-IV77
Plasmid#244906PurposeRep/Cap plasmid for producing variant AAV-hCA4-IV77DepositorInsertSynthetic construct producing AAV REP/CAP
UseAAVExpressionMammalianAvailable SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(mem).iGlucoSnFR2
Plasmid#244088PurposePlasma membrane targeted expression of green glucose sensorDepositorInsertiGlucoSnFR2
UseAAVTagsIgG kappa leaderAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(cyto).iGlucoSnFR2
Plasmid#244111PurposeCytosolic expression of green glucose sensorDepositorInsertiGlucoSnFR2
UseAAVAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
NanoBiT Assay Vector, HER1(1-668)-LgBiT
Plasmid#237104PurposeExpress HER1(1-668)-LgBiT Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertHER1 (EGFR Human)
TagsLgBiTExpressionMammalianMutation1-668PromoterCMVAvailable SinceSept. 16, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Rat SARE-Arc Min Promoter-Flag Arc-SV40 3'UTR
Plasmid#233057PurposeTo express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains a SV40 3' UTRDepositorAvailable SinceAug. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-USP7-sh1
Plasmid#235527PurposeshRNA against human USP7DepositorInsertUSP7 (USP7 Human)
UseLentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-USP7-sh5
Plasmid#235528PurposeshRNA against human USP7DepositorInsertUSP7 (USP7 Human)
UseLentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
RNF168 C-terminal sgRNA
Plasmid#207100PurposepX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.DepositorInsertGAGATGCACAAAGTAAGGCC
ExpressionMammalianPromoterCMV and U6Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2_Rluc-Y0x-Fluc
Plasmid#233614PurposeExpression of Rluc-Fluc reporter gene containing no YDepositorInsertRluc-Y0x-Fluc
UseLuciferaseAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
SeqCBE
Plasmid#232115PurposeExpresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP
Plasmid#232608PurposePan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTRDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-5xQUAS+Δpes-10P::AI::eTsChR
Plasmid#232612PurposeExpression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric proteinDepositorInserteTsChR
ExpressionWormMutationN/AAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Aquamarine-LC3B G120A-TdLanYFP
Plasmid#228561PurposeEncodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoterDepositorAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CRISPRi-sgRNA
Plasmid#221135PurposeHelper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with dCas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-CRISPRi
Plasmid#221136PurposeCoxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.DepositorInsert3xF-dCas9
UseCRISPRTags3xFLAGExpressionBacterialPromotercbu1169 promoterAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-V5-n2StayGoldC4 (JDW 1354)
Plasmid#224486PurposeGateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)DepositorInsertH2B-V5-n2StayGoldC4
UseGateway cloningAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pET-6xHis-GFP(0)-L1
Plasmid#205033PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-L1
TagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB4
Plasmid#200983PurposeMammalian expression plasmid for myc-tagged EphB4DepositorInsertEphB4 (EPHB4 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5mycGW-Eps8L2
Plasmid#196542PurposeExpression of myc-Eps8L2-FLDepositorAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Clover4-LUMABS-S1 RBD
Plasmid#183491PurposeTo express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.DepositorInsertClover4-LUMABS-S1 RBD
ExpressionInsectAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRG272-eya
Plasmid#99085PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)DepositorInserteyes absent (eya)
UseT/a cloning vector for dsrna generation and the g…Available SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG217-soxB
Plasmid#99086PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea soxB (also known as soxB1-1)DepositorInsertsoxB (also known as soxB1-1) in situ probe
UseT/a cloning vector for dsrna generation and the g…Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST MTFR1L-V5
Plasmid#69822PurposeExpresses MTFR1L-V5 in mammalian cellsDepositorInsertMitochondrial fission regulator 1-like (MTFR1L Human)
UseRetroviralTagsv5ExpressionMammalianPromotercmvAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-FLNa WT
Plasmid#8982DepositorAvailable SinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pX459-SpCas9-NG
Plasmid#171370PurposeSpCas9-NG-mediated genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 for sgRNA; CBh for Cas9-2A-puroRAvailable SinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
FHRE-Luc
Plasmid#1789PurposeReporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
CRBN_ΔHBD
Plasmid#232779PurposeExpresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domainDepositorInsertCereblon (CRBN Human)
TagsMBP-His6-TEVExpressionBacterialMutationCRBN (residues 47 to 193 and 249 to 436) with GNG…PromoterT7Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-CAV
Plasmid#168303PurposeChemogenetic fluorescent H2O2 reporter targeted to the caveolaeDepositorInsertHyPer7.2DAAO-CAV
ExpressionMammalianPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM512
Plasmid#140192PurposeFor curing of Orbit-integrated plasmids and associated drug markerDepositorInsertszeocin resistance gene
Bxb1gp47 (directionality factor)
ExpressionBacterialMutationCloned in gp47 directinality factor in place of R…PromoterEM7 and PtetAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL410_INS421
Plasmid#49057PurposeExpresses luciferase under control of human insulin gene promoterDepositorAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcrDNA3.1-PRKACA
Plasmid#100890PurposeMammalian expression of PRKACADepositorInsertProtein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
ExpressionMammalianMutationIsoform 1 wild typePromoterCMVAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1-CMV-mito-DAAO
Plasmid#141132PurposeExpresses DAAO with mitochondrial localization signal in mammalian cellsDepositorInsertmito-DAAO
Tags2 mitochondrial localization signals and 7xHisExpressionMammalianMutationdeleted DAAO amino acids 367-368PromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Synapsin-cyto-mRuby3-iATPSnFR1.0
Plasmid#102557Purposeexpresses cytoplasmic ATP sensor in neurons with red reference proteinDepositorInsertmRuby3-iATPSnFR1.0
UseAAVTagsRuby3Available SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3868 HA-E6-AP
Plasmid#8648DepositorAvailable SinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only