161,674 results

1. pEF6.mCherry-TSG101

   Plasmid

   #38318

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   TSG101 (TSG101 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. M13KO7-ΔPS-ΔgeneIII

   Plasmid

   #134351

   Purpose

   Helper phage for M13 phage production

   Depositor

   Mark Isalan

   Article

   Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

   Insert

   Helper phage gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

   Plasmid

   #194683

   Purpose

   CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_05-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SRC_HUMAN_D0

   Plasmid

   #79700

   Purpose

   This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SRC (SRC Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-6xHis-MBP-TEV site-TadA8.20

   Plasmid

   #194702

   Purpose

   Expresses N-terminal MBP fused TadA8.20 in bacteria

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   TadA8.20
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-FOXO1A

   Plasmid

   #153141

   Purpose

   Expression of Flag tagged human FOXO1A in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXO1 (FOXO1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GCN5 core-dCas9-p300 core

   Plasmid

   #179553

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.MTF2

   Plasmid

   #125169

   Purpose

   Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   MTF2 (MTF2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YIplac211-ABP1-iGFPx6

    Plasmid

    #177099

    Purpose

    Expresses Abp1-iGFPx6 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    ABP1 (ABP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV-mNINJ1-GFP

    Plasmid

    #208776

    Purpose

    MSCV vector expressing WT mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. myc-tagged NRP1 (human)

    Plasmid

    #213068

    Purpose

    Express myc-NRP1 in mammalian cells

    Depositor

    Yoav Henis

    Insert

    neuropilin 1 (NRP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    N-terminal myc tag was introduced by a three-step…

    Promoter

    T7

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-Aurora A

    Plasmid

    #198402

    Purpose

    Expression of EGFP-tagged Aurora A in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    Aurora A
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCh-Mfn2

    Plasmid

    #141156

    Purpose

    Mammalian expression and labeling mitofusin 2

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 2 (MFN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmGFP11C-­Tau

    Plasmid

    #71434

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Use

    Tags

    GFP (213-228)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. TFORF0537

    Plasmid

    #141639

    Purpose

    Lentiviral vector for overexpressing the PAX2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PAX2 (PAX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLQ1651-sgTelomere(F+E)

    Plasmid

    #51024

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    Optimized sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFG2

    Plasmid

    #87250

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Use

    Tags

    parS segments

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mPlum-LaminB1-10

    Plasmid

    #55981

    Purpose

    Localization: Nuclear Envelope, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-DsRed_IRES_SNCA:EGFP

    Plasmid

    #92195

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-SNCA-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA5-FRT/TO-eGFP-53BP1

    Plasmid

    #60813

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. TFORF0692

    Plasmid

    #143064

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF0689

    Plasmid

    #141662

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF4G (HNF4G Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-mKelly2

    Plasmid

    #119783

    Purpose

    Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly2
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR221_SLC1A2

    Plasmid

    #131901

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A2 (SLC1A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pHAGE-PIK3CA

    Plasmid

    #116771

    Purpose

    Lentiviral expression of PIK3CA

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. acGFP WC visual barcode

    Plasmid

    #158672

    Purpose

    tagging clonal populations with whole cell acGFP fluorescent protein

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    acGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJC175e

    Plasmid

    #79219

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PPSP gIII, luxAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPSP

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ER-RA

    Plasmid

    #209872

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSCVpuro-tdTomato

    Plasmid

    #97079

    Purpose

    Retroviral plasmid containing tdTomato for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB-TO-NGN2-EMX1 BSD-mApple

    Plasmid

    #182311

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
    bsd-T2A-mycNLS-mApple
    

    Use

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDD426

    Plasmid

    #202079

    Purpose

    Frame +1/-0 selector for NHEJ knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDRF1-GW yAT1.03R122KR126K

    Plasmid

    #132782

    Purpose

    Non-binding version of the FRET biosensor for ATP measurements in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

    Insert

    yAT1.03R122KR126K ymTq2-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R122K, R126K

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lyn-R-GECO1

    Plasmid

    #120410

    Purpose

    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

    Depositor

    Won Do Heo

    Article

    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

    Insert

    Lyn-R-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. T4 td upstream intron-PABP

    Plasmid

    #189972

    Purpose

    Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    T4 td upstream intron-PABP
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmGFP10C-­Tau

    Plasmid

    #71433

    Purpose

    Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

    Depositor

    Henri Huttunen

    Article

    Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

    Insert

    Tau (0N4R) (MAPT Human)
    

    Use

    Tags

    GFP (1-212)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pSCAR_Cas9-blast_GFP

    Plasmid

    #162074

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-blasticidin S deaminase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. plYJP022-int7

    Plasmid

    #202287

    Purpose

    Plasmid containing integrase for LPattB7 site

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    int7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP-NUP188

    Plasmid

    #159893

    Purpose

    Expresses NUP188 in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

    Insert

    NUP188 (NUP188 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    To avoid NotI site, first three amino acids are d…

    Promoter

    CMV

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti CMV Blast empty (w263-1)

    Plasmid

    #17486

    Purpose

    3rd gen lentiviral empty control vector, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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45. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid

    #219564

    Purpose

    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

    Depositor

    Andrea Ventura

    Article

    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

    Insert

    3` CC: Hygro – GFP-N – loxP – mScarlet
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. TFORF1898

    Plasmid

    #144116

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PATZ1 (PATZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLenti6-V5-Topo mCherry

    Plasmid

    #128062

    Purpose

    Lentiviral vector for C terminal mCherry fusions

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pHR-dSV40-CTLA-4-mGFP

    Plasmid

    #223594

    Purpose

    Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMV306hsp+LuxG13

    Plasmid

    #26161

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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