19,207 results

1. ClopHensorN

   Plasmid

   #50758

   Purpose

   Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

   Depositor

   Colin Akerman

   Article

   Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

   Insert

   ClopHensorN
   

   Use

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Promoter

   CAG

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR_SFFv_4D5-WT-Highest-CAR_RHL004

   Plasmid

   #164826

   Purpose

   Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFUGW-H1 empty vector

   Plasmid

   #25870

   Purpose

   3rd generation lentiviral vector

   Depositor

   Sally Temple

   Article

   Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

   Plasmid

   #61592

   Purpose

   A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti HRE-Luc pGK Hygro

   Plasmid

   #118706

   Purpose

   reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HRE (HIF-responsive promoter (3xHRE)-luc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLIK DN RhoA

   Plasmid

   #84646

   Purpose

   Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   RhoA (RHOA Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Dominant Negative

   Promoter

   Available since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

8. TRIPZ-bioGEF-UBwt-PURO

   Plasmid

   #208045

   Purpose

   Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   Ub (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDH-EF1-DIO-copGFP

   Plasmid

   #72253

   Purpose

   Express copGFP under EF-1 promoter when Cre is expressed by another vector

   Depositor

   Kazuhiro Oka

   Insert

   copGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   elongation factor-1

   Available since

   Jan. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCiVSP-SD64TF

    Plasmid

    #80332

    Purpose

    Expresses Ciona VSP in Xenopus Oocyte

    Depositor

    Yasushi Okamura

    Article

    Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

    Insert

    Ciona intestinalis voltage-sensing phosphatase (vsp Ciona intestinalis)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PCDNA3-GFP1-9 T2A mCherry

    Plasmid

    #124430

    Purpose

    Expresses GFP1-9 and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9 T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHDM-Hgpm2

    Plasmid

    #164441

    Purpose

    Encodes codon-optimized HIV gagpol for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    Codon Optimized HIV-1 GagPol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTP2620

    Plasmid

    #113311

    Purpose

    human Rad50 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Rad50 (RAD50 Human)
    

    Use

    Tags

    HA, 6xhis

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pg-HIF-1alpha-EGFP

    Plasmid

    #87204

    Purpose

    C-terminally EGFP-tagged human HIF-1a

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-1a (HIF1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L40F, M88V, Q814R

    Promoter

    CMV

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRL SFFV d20GFP.T2A.mTagBFP Donor

    Plasmid

    #31485

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d20BFP T2A BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    GFP has 20 amino acids deleted from the C terminu…

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTP813

    Plasmid

    #113308

    Purpose

    human Mre11 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

    Insert

    Mre11 (MRE11 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-Tev-UbE1

    Plasmid

    #186804

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE1 (UBA1 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-FKBP-EGFP-HOTag3

    Plasmid

    #106924

    Purpose

    Expresses FKBP-EGFP-HOTag3 in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    FKBP-EGFP-HOTag3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-SENP8

    Plasmid

    #18066

    Depositor

    Edward Yeh

    Article

    Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

    Insert

    SENP8 (SENP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

21. Watermelon backbone

    Plasmid

    #155258

    Purpose

    Can be used to generate a Watermelon library

    Depositor

    Joan Brugge, Aviv Regev

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKL2299

    Plasmid

    #186332

    Purpose

    This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

    Depositor

    Kan Wang

    Article

    Kang et al Front Plant Sci. 2022 May 4;13:860971. d

    Insert

    virulence genes from Agrobacterium tumefaciens pTiBo542
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hCathepsin B

    Plasmid

    #11249

    Purpose

    Mammalian expression of human cathepsin B

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2005 Dec 8. ():.

    Insert

    cathepsin B (CTSB Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pKMyc

    Plasmid

    #19400

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Type

    Empty backbone

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBB535

    Plasmid

    #27392

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    dnaK, dnaJ (dnaJ, dnaK E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Tet-O-FUW-Brn2

    Plasmid

    #27151

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQE-60NA-msGFP2

    Plasmid

    #135301

    Purpose

    Encodes a photostable monomeric superfolder GFP

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    msGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Aequorea victoria, Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Use

    Tags

    P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFlag-CMV1-hMD2

    Plasmid

    #13028

    Depositor

    Doug Golenbock

    Insert

    MD2 (LY96 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge