159,258 results

1. pLentiCRISPRv2 sgIRF3#5

   Plasmid

   #127642

   Purpose

   Knock-out of human IRF3

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 sgRNA (IRF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE

   Plasmid

   #106303

   Purpose

   The plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imaging

   Depositor

   James Zhe Liu

   Article

   Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

   Insert

   scFv-GCN4-HaloTag-GB1-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p5xUAS-Flag-NPM1wt

   Plasmid

   #131822

   Purpose

   4-hydroxytamoxifen inducible vector expressing Flag-NPM1wildtype (human) in mammalian cells through transduction

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3 Flag-humanTRAF6 (1093)

   Plasmid

   #66929

   Purpose

   Mammalian Expression of TRAF6

   Depositor

   Michael Karin

   Insert

   TRAF6 (TRAF6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-GGamma2-GFP2

   Plasmid

   #166774

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma2-GFP2 (GNG2 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

   Plasmid

   #196703

   Purpose

   Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 D210N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D210N, catalytically inactive

   Promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-DIO-dAPEX2

   Plasmid

   #117174

   Purpose

   Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   dAPEX2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NC16 pCDNA3.1 FLAG NRF2

    Plasmid

    #36971

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSK9065

    Plasmid

    #84345

    Purpose

    tightly controlled expression of mRFP fusion proteins in S. aureus

    Depositor

    Neville Firth

    Article

    Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

    Type

    Empty backbone

    Use

    Tags

    mRFPmars

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl/tet

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. VGAT scFv [L118/14]

    Plasmid

    #206754

    Purpose

    Mammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    VGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF0022

    Plasmid

    #141481

    Purpose

    Lentiviral vector for overexpressing the IRX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRX3 (IRX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-HisD-miniGFP1

    Plasmid

    #188896

    Purpose

    Express 6xHis-Tag miniGFP1 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-BAF

    Plasmid

    #101772

    Purpose

    Expresses EGFP tagged BAF in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30a(+)-PE2

    Plasmid

    #180015

    Purpose

    Expressing PE2 protein in E.coli for protein purification

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lac operator-controlled T7 promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-AMBRA1-3xFLAG

    Plasmid

    #172605

    Purpose

    Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IFP2.0-N1

    Plasmid

    #54785

    Purpose

    Localization: N1 Cloning Vector, Excitation: 684, Emission: 708

    Depositor

    Michael Davidson, Xiaokun Shu

    Article

    Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    IFP2.0

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pHR_SFFV

    Plasmid

    #79121

    Purpose

    Lentiviral vector for constitutive expression of genes (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CIp10 - γmGFP (γmGFP based)

    Plasmid

    #163119

    Purpose

    Expresses in Candida albicans

    Depositor

    Patrick Van Dijck

    Article

    Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

    Insert

    gamma mGFP
    

    Use

    Candida albicans expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAV1K-T5-gfp

    Plasmid

    #26702

    Depositor

    Ichiro Matsumura

    Article

    Bryksin et al PLoS One. 2010 . 5(10):e13244.

    Insert

    T5lacOlacOeGFP biobrick
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. VEGFR2-mCh

    Plasmid

    #108854

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Labeled with mCherry on the C-Terminus via a 3 am…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti PGK Blast V5-LUC (w528-1)

    Plasmid

    #19166

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXPG

    Plasmid

    #71248

    Purpose

    improved luciferase reporter gene plasmid

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDest-565

    Plasmid

    #11520

    Purpose

    Gateway destination vector for bacterial expression; His,GST tag

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAVS1-pur-CAG-mCherry

    Plasmid

    #159458

    Purpose

    AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GST-RH▲-MNase

    Plasmid

    #136292

    Purpose

    Expression of catalytic inactive RNase H fused to micrococcal nuclease

    Depositor

    Kavitha Sarma

    Article

    Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

    Insert

    RNase HI-D10R-E48R and MNase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEx-deletion-erm

    Plasmid

    #172213

    Purpose

    For allelic exchange in erythromycin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Allelic exchange in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MRGPRX2-Tango

    Plasmid

    #66440

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MRGPRX2 (MRGPRX2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-8xHis-Ub

    Plasmid

    #107392

    Purpose

    Mammalian expression 8xHis-Ub

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    8 tandem repeats of 6xHis-Ub (See comments below)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid

    #52421

    Purpose

    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

    Depositor

    Bertrand Collet

    Insert

    Fusion NLS-EGFP-P2A-Cherry-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBPGAL4.2::VP16Uw

    Plasmid

    #26228

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL4::VP16 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTRE3G-Bi-α-chain

    Plasmid

    #133730

    Purpose

    Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    a-Chain_gePSI
    ZsGreen1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Only one part of the gePSI-system (aa1-141 + stop…

    Promoter

    pTRE3G-Bi

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV-CamKII-TRPV1-P2A-mCherry

    Plasmid

    #200829

    Purpose

    Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-mCherry (Trpv1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA-hDDB2-WT-V5

    Plasmid

    #167468

    Purpose

    ectopic expression of hDDB2 in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

    Insert

    DDB2 (DDB2 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #210523

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA-directcapture
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188779

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

    Plasmid

    #170163

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-P2A-H2B-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCM66

    Plasmid

    #45827

    Depositor

    Mary Lidstrom

    Article

    Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

    Type

    Empty backbone

    Use

    Broad host range cloning vector in bacteria

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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46. mKikGR-C1

    Plasmid

    #54656

    Purpose

    Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Habuchi et al PLoS One. 2008;3(12):e3944. doi: 10.1371

    Type

    Empty backbone

    Use

    Tags

    mKikGR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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47. pRL528

    Plasmid

    #58495

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al Methods Enzymol. 1988;167:747-54.

    Insert

    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. TFORF3527

    Plasmid

    #145003

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ERF (ERF Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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