Skip to main content
Addgene
Showing: 601 - 640 of 156664 results

  1. pBSJL1

    Plasmid
    #71261
    Purpose
    E. coli-Veillonella shuttle plasmid
    Depositor
    Felicia Qi
    Article
    Liu et al Appl Environ Microbiol. 2012 May;78(9):3
    Type
    Empty backbone
    Use
    E. coli-veillonella shuttle plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. TRE-TDP-43ΔNLS-mClover3

    Plasmid
    #214916
    Purpose
    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43
    Depositor
    Ophir Shalem
    Article
    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d
    Insert
    TDP-43ΔNLS (TARDBP Human)
    Use
    Lentiviral
    Tags
    mClover3
    Expression
    Mutation
    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214916-plasmid-map-sequence-id-424951

    Enlarge

  3. TFORF2891

    Plasmid
    #142169
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GLI1 (GLI1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142169-plasmid-map-sequence-id-397695

    Enlarge

  4. TFORF2693

    Plasmid
    #142359
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD4 (TEAD4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142359-plasmid-map-sequence-id-397176

    Enlarge

  8. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159752-plasmid-map-sequence-id-313824

    Enlarge

  9. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

    Enlarge

  11. pAAV-flex-GFP-shRNA-GluN1

    Plasmid
    #182502
    Purpose
    Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182502-plasmid-map-sequence-id-355962

    Enlarge

  12. mApple-LaminB1-10

    Plasmid
    #54917
    Purpose
    Localization: Nuclear Envelope, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

    Enlarge

  21. lucMAPT-GenRep

    Plasmid
    #214674
    Purpose
    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Use
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…
    Promoter
    CMV
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214674-plasmid-map-sequence-id-424937

    Enlarge

  26. pETM33_Nsp10

    Plasmid
    #156479
    Purpose
    Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156479-plasmid-map-sequence-id-307731

    Enlarge

  27. pKL

    Plasmid
    #110741
    Purpose
    Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site
    Depositor
    Tim Richmond
    Article
    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin and p10 promoters
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110741-plasmid-map-sequence-id-213581

    Enlarge

  30. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. hSyn-DIO-somBiPOLES-eGFP

    Plasmid
    #192584
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192584-plasmid-map-sequence-id-379290

    Enlarge

Showing: 601 - 640 of 156664 results