Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

170,794 results

Showing: 601 - 640 of 170794 results

  3. pSRG100 (germline optimized 3xGCN4 for C. elegans)

    Plasmid
    #246457
    Purpose
    Encodes germline optimized 3xGCN4 for C. elegans. Useful to make CRISPR/Cas9 repair templates for 3xGCN4 integration in a gene of interest. For SunTag live-imaging of translation or protein labelling.
    Depositor
    Suzan Ruijtenberg
    Insert
    3xGCN4 (GLO)
    Expression
    Worm
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    246457-plasmid-map-sequence-id-496369

    Enlarge

  4. pSRG101 (KasI::24xGCN4::T2A::AvrII)

    Plasmid
    #246458
    Purpose
    24xGCN4::T2A with flanking restriction sites (KasI and AvrII). Useful for cloning SunTag reporter plasmids for expression in C. elegans.
    Depositor
    Suzan Ruijtenberg
    Insert
    24xGCN4::T2A
    Expression
    Bacterial
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    246458-plasmid-map-sequence-id-496351

    Enlarge

  6. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα

    Plasmid
    #242411
    Purpose
    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).
    Depositor
    Ewan St. John Smith
    Article
    Lim et al bioRxiv 2024.09.24.614636
    Inserts
    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-srIκBα
    Use
    Mouse Targeting; Self-amplifying rna
    Expression
    Mammalian
    Mutation
    serines 32 and 36 to alanines
    Available Since
    Sept. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242411-plasmid-map-sequence-id-487192

    Enlarge

  7. CMV/CRAF-SmBiT/BlastR Vector

    Plasmid
    #238811
    Purpose
    Express CRAF-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CRAF (RAF1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238811-plasmid-map-sequence-id-479920

    Enlarge

  8. CMV/BRAF-SmBiT/BlastR Vector

    Plasmid
    #238813
    Purpose
    Express BRAF-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    BRAF (BRAF Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238813-plasmid-map-sequence-id-479921

    Enlarge

  9. CMV/BRAF-LgBiT/HygR Vector

    Plasmid
    #238814
    Purpose
    Express BRAF-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    BRAF (BRAF Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238814-plasmid-map-sequence-id-479837

    Enlarge

  10. SAGA005

    Plasmid
    #240754
    Purpose
    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15A J23100 BCD_medium - gfp - tetAp15A- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240754-plasmid-map-sequence-id-483915

    Enlarge

  23. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

    Enlarge

  29. rtTA-N144

    Plasmid
    #66810
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance
    Depositor
    Andrew Yoo
    Article
    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi
    Insert
    Reverse tetracycline-controlled transactivator
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available Since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    66810-plasmid-map-sequence-id-418923

    Enlarge

  30. AVPR2-DuET

    Plasmid
    #213188
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR2 (AVPR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213188-plasmid-map-sequence-id-430753

    Enlarge

  31. AVPR1A-DuET

    Plasmid
    #213186
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR1A (AVPR1A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    213186-plasmid-map-sequence-id-431750

    Enlarge

  32. AVPR1B-DuET

    Plasmid
    #213187
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    AVPR1B (AVPR1B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213187-plasmid-map-sequence-id-430752

    Enlarge

  35. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

    Enlarge

  37. eeBxb1_IVT

    Plasmid
    #222342
    Purpose
    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template
    Depositor
    David Liu
    Article
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    Template for in vitro transcription
    Promoter
    T7 (inactivated)
    Available Since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222342-plasmid-map-sequence-id-440249

    Enlarge

Showing: 601 - 640 of 170794 results