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169,661 results

Showing: 8151 - 8200 of 169661 results

  7. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid
    #103849
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    CMV
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103849-plasmid-map-sequence-id-199152

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  19. LPAR2-DuET

    Plasmid
    #213330
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR2 (LPAR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213330-plasmid-map-sequence-id-430864

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  22. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Ivan Dikic
    Article
    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2
    Insert
    Cereblon (CRBN Human)
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232779-plasmid-map-sequence-id-462151

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  23. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90979-plasmid-map-sequence-id-180505

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  24. pDEST-PB-R4R3

    Plasmid
    #139518
    Purpose
    Provides two PiggyBac transposon sequences between gene cassettes in the destination vector
    Depositor
    Minoru Ko
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139518-plasmid-map-sequence-id-274611

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  37. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Larry Gerace
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175108-plasmid-map-sequence-id-351795

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  38. pNL [NlucP/XRE/Hygro] Vector

    Plasmid
    #236927
    Purpose
    Express XRE in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    XRE
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236927-plasmid-map-sequence-id-474671

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  40. PSMC2_GFP_Cterm

    Plasmid
    #221516
    Purpose
    Fluorescently tagged Psmc2-GFP
    Depositor
    Alessandro Ori
    Insert
    PSMC2 (PSMC2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221516-plasmid-map-sequence-id-439441

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  43. pcDNA-RLuc8

    Plasmid
    #87121
    Purpose
    Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.
    Depositor
    Sanjiv Sam Gambhir
    Article
    Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-
    Insert
    Renilla Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…
    Promoter
    CMV
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87121-plasmid-map-sequence-id-175252

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  46. mCherry-Calreticulin-N-16

    Plasmid
    #55006
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55006-plasmid-map-sequence-id-470324

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  47. pcDNA-GFP-STAT1

    Plasmid
    #11987
    Depositor
    Steven Johnson
    Insert
    Signal transducer and activator of transcription 1 (STAT1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    A188T, Q718R
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11987-plasmid-map-sequence-id-483719

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  48. GRM2-DuET

    Plasmid
    #213301
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM2 (GRM2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213301-plasmid-map-sequence-id-430907

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  49. AD59_pEP.DonorCLYBL.TS

    Plasmid
    #199227
    Purpose
    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199227-plasmid-map-sequence-id-391392

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Showing: 8151 - 8200 of 169661 results