162,408 results
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MGAT-SEP-mRuby3
Plasmid#200937Purposecis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Popt
Plasmid#231705PurposeMammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV P gene (codon-optimized )
UseVsv expressionTagsExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lopt
Plasmid#231706PurposeMammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV L gene (codon-optimized )
UseVsv expressionTagsExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Mito-CanlonicSF/pCMV-myc-mito
Plasmid#224465PurposeTo explore mitochondrial lactate dynamicsDepositorInsertCanlonicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
12XCSL-d1EGFP
Plasmid#47684PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsd1EGFPExpressionMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Lifeact-EGFP
Plasmid#67303PurposeFluorescent indicator for F-actinDepositorInsertLifeact-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Blast-SHMT2-3xFLAG
Plasmid#233891Purposelentiviral overexpression vector for SHMT2DepositorInsertSHMT2 (SHMT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO human shRNA 1 DEPTOR
Plasmid#21335DepositorInsertDEPTOR shRNA 1 (DEPTOR Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
DH-BB(5'SIN;TE12ORF)
Plasmid#72309PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virusDepositorInsertDH-BB(5'SIN;TE12ORF)
UseSindbis virus helper constructTagsExpressionMutationPromoterAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING
Plasmid#102586PurposeLentivector to express STING and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008
Plasmid#187916PurposeExpresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 2
UseTags3xFLAGExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
XRE-H2B-mScarlet-I
Plasmid#182296PurposeFluorescent reporter for AhR activityDepositorInsertmScarlet-I
UseTagsm-Scarlet-IExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTetO-hOSK-CASH-1
Plasmid#188977PurposeA CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.DepositorInsertDoxycycline inducible reprogramming donor cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
Plasmid#160540PurposeExpresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.DepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZapLck biosensor
Plasmid#131584PurposeLck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRETDepositorInsertZapLck biosensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Bcatfl
Plasmid#24193DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG
Plasmid#187179PurposeExpresses green norepinephrine indicator nLightG in neuronal cellsDepositorHas ServiceAAV9InsertnLightG
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
BiP-mGFP
Plasmid#62231PurposeEncodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.DepositorInserthamster BiP-mGFP-KDEL
UseTagsmGFPExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-AURKA
Plasmid#23532DepositorInsertAURKA (AURKA Human)
UseGateway donor vectorTagsExpressionMutationI57VPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-GPAT4152-208
Plasmid#134468PurposeGPAT4152-208 expressionDepositorInsertGPAT4 (GPAT4 Human)
UseTagsExpressionMammalianMutationGPAT4, amino acids 152-208PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
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ABE8.20-NG
Plasmid#226857PurposeExpresses ABE8.20-NG base editor in mammalian cells with eGFP markerDepositorInsertABE8.20-NG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry2(1-10)
Plasmid#82603PurposeLentiviral vector for constitutive expression of sfCherry2(1-10)DepositorInsertsfCherry2(1-10)
UseLentiviralTagsExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterSFFVAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV LIC cloning vector (2S-T)
Plasmid#29711DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-VE-Cadherin-N-10
Plasmid#58142PurposeLocalization: Tight Junctions, Excitation: 554, Emission: 581DepositorInsertVE-Cadherin (CDH5 Human)
UseTagstdTomatoExpressionMammalianMutationI503T in VE-CadherinPromoterCMVAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vinculin-23
Plasmid#55159PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertVinculin (VCL Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shRen.713
Plasmid#186712PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).DepositorInsertshRen.713
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-ChrimsonR-tdTomato
Plasmid#99231PurposeAAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterCaMKIIAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN7HELIX
Plasmid#190662PurposeExpresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
UseTagsExpressionMammalianMutationnAniI = K227M, F80K, L232KPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-HA-mNG21-10
Plasmid#141165PurposeSplit Fluorescent Reporter for ER Retrograde TraffickingDepositorInsertER-HA-mNG21-10-KDEL
UseLentiviralTagsSignal Sequence, KDEL and HAExpressionMammalianMutationK128M, S142T, R150M, G172V and K213MPromoterEf1aAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3007
Plasmid#144483PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD1 (SMAD1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)
Plasmid#217345PurposecrRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81DepositorInsertscrCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX2-Tango
Plasmid#66440PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMRGPRX2 (MRGPRX2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCPP3373
Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
UseTagsExpressionBacterialMutationSynonymous point mutation in the wobble position…PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
GPR120-DuET
Plasmid#213261PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR120 (FFAR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeon-Vamp2
Plasmid#186619PurposeEncodes Vamp 2 fused to mNeonGreenDepositorInsertVesicle Associated Membrane Protein 2 (VAMP2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1218
Plasmid#142157PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPRRX1 (PRRX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TurboID-mNeongreen-3xFLAG fusion pos3
Plasmid#172864PurposeC. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion proteinDepositorInsertTurboID
UseUnspecifiedTagsFLAG, mNeongreen, and mycExpressionMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato
Plasmid#234952PurposeHuman codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-R-Creon shRNA[Control]
Plasmid#180775PurposeThis vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.DepositorTypeEmpty backboneUseAAV, Cre/Lox, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hGOLGA2-C-PEN2
Plasmid#183649PurposeLentiviral expression of a golgi-residential PEN2.DepositorUseLentiviralTagsHAExpressionMutationWTPromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2164
Plasmid#142186PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGRHL2 (GRHL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sniper2L
Plasmid#193856PurposeExpresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-Bmpr1A-ca
Plasmid#217536PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptorDepositorArticleInsertconstitutively active BMP receptor 1a (BMPR1A Human)
UseAAVTagsHAExpressionMammalianMutationPromoterTet operon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-8xbiRhoBAST-24xMS2v6
Plasmid#196352PurposeExpression of mAzurite mRNA with 8xbiRhoBAST-24xMS2v6 in 3'UTRDepositorInsertmAzurite-8xbiRhoBAST-24xMS2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHScarlet
Plasmid#191258PurposeBacterial expression plasmids for pH sensitive fluorescent protein pHScarletDepositorInsertpHScarlet
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only
