8,866 results

1. pUC19

   Plasmid

   #50005

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   lac

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1aH N90-beta-catenin

   Plasmid

   #86499

   Purpose

   Mammalian expression of Myc tagged N90 beta-catenin

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90; constitutively acti…

   Promoter

   EF1a

   Available Since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRIS-PITChv2-Puro-dTAG (BRD4)

   Plasmid

   #91793

   Purpose

   PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   Puro-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage UbiC G3BP1-GFP-GFP

   Plasmid

   #119950

   Purpose

   Lentiviral vector expressing G3BP1-GFP fusion protein

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-2xFYVE

   Plasmid

   #140050

   Purpose

   Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available Since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-LYC

   Plasmid

   #53270

   Purpose

   Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

   Insert

   crtE, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Luciferase-pcDNA3

   Plasmid

   #18964

   Depositor

   William Kaelin

   Article

   Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

   Insert

   Firefly Luciferase
   

   Expression

   Mammalian

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. tetO.Nfib.Hygro

   Plasmid

   #117271

   Purpose

   Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfib (Nfib Mouse)
   

   Use

   Lentiviral

   Tags

   IRES and hygromycin resistant cassette (C termina…

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-IRES-Puro

   Plasmid

   #85132

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. tetO.Sox9.Puro

    Plasmid

    #117269

    Purpose

    Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A and puromycin resistant cassette (C terminal …

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV SCH9

    Plasmid

    #212708

    Purpose

    A SCHEMA evolved AAV variant effective at targeting subventricular NSCs

    Depositor

    David Schaffer

    Article

    Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi:

    Insert

    AAV2 Rep SCH9 Cap
    

    Use

    AAV

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCTcon2

    Plasmid

    #41843

    Depositor

    Dane Wittrup

    Article

    Chao et al Nat Protoc. 2006;1(2):755-68.

    Insert

    CD20
    

    Tags

    HA and c-myc

    Expression

    Yeast

    Available Since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Myd88-CFP

    Plasmid

    #13026

    Depositor

    Doug Golenbock

    Insert

    myeloid differentiation primary response gene (88) (MYD88 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTagBFP2-pBAD

    Plasmid

    #54572

    Purpose

    Localization: Protein Expression Vector , Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, T7, and mTagBFP2

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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