19,207 results

1. pcDNA 3.1 SIRT1-FL

   Plasmid

   #105670

   Purpose

   Mammalian expression construct of mouse SIRT1 full length isoform

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCI SMN1

   Plasmid

   #72286

   Purpose

   SMN1 exon 7 splicing cassette

   Depositor

   Elliot Androphy

   Article

   Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

   Insert

   SMN1 (SMN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   exons 6-7-8 including introns

   Promoter

   CMV

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. VN-Tau (wt)

   Plasmid

   #87368

   Purpose

   expresses hTau fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBECKP-spe

   Plasmid

   #117236

   Purpose

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFPC1-human APPL1

   Plasmid

   #22198

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mEmerald-Keratin-17

   Plasmid

   #54134

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pAAVS1-TRE-MA4-GFP

   Plasmid

   #114380

   Purpose

   Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

   Depositor

   Yuet Wai Kan

   Article

   Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   MLL-AF4 fusion gene (AFF1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV hUbC-dCas9-T2A-GFP

   Plasmid

   #53191

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-Paxillin WT

   Plasmid

   #186152

   Purpose

   For expressing paxillin variants in mammalian cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PXN (PXN Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p_m16S

    Plasmid

    #160549

    Purpose

    Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline

    Depositor

    Denis Gerlier

    Article

    Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do

    Insert

    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HR180-LGR5-GFP

    Plasmid

    #129092

    Purpose

    KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    EGFP and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mTLR4 flag

    Plasmid

    #13087

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (Tlr4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-19 have been replaced with mammalia…

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-Oct3/4-IRES-Neo

    Plasmid

    #13461

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P31S

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFPC1-hMyoX

    Plasmid

    #47608

    Depositor

    Emanuel Strehler

    Article

    Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

    Insert

    MyoX (MYO10 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. N-Myc-53BP1 WT pLPC-Puro

    Plasmid

    #19836

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Oct 19. ():.

    Insert

    p53 Binding Protein 1 (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid

    #129716

    Purpose

    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6972 pPROEX-HTb-SSA1

    Plasmid

    #1231

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Ssa1 (SSA1 Budding Yeast)
    

    Use

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 9, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pUC18T-mini-Tn7T-Tp-dsRedExpress

    Plasmid

    #65036

    Purpose

    TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAuroraA-GFP-AURKA-mCherry

    Plasmid

    #99878

    Purpose

    Expression of GFP-AURKA-mCherry in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    AURKA minimal

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBABE-puro TPR-Met

    Plasmid

    #10902

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    TPR-Met (MET, TPR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Constitutively active form of the c-Met receptor

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWPXLd

    Plasmid

    #12258

    Depositor

    Didier Trono

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. bRA89

    Plasmid

    #100950

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRPF185

    Plasmid

    #106367

    Purpose

    C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

    Insert

    Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    codon optimised for C. difficile

    Promoter

    Ptet

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-SADB19N

    Plasmid

    #32630

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    B19N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-VP6SA11

    Plasmid

    #89166

    Purpose

    Express Rotavirus SA11 VP6 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SRE reporter vector_559

    Plasmid

    #82686

    Purpose

    Cloning vector containing serum response element (SRE) followed by an AscI restriction site

    Depositor

    Harald Janovjak

    Article

    Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Nano-lantern/pcDNA3

    Plasmid

    #51970

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-hA3B-BE3

    Plasmid

    #113411

    Purpose

    Expresses hA3B-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3B-BE3 (APOBEC3B S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-TAT-1-101-FLAG

    Plasmid

    #35981

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1 101 amino acids

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

    Plasmid

    #69100

    Purpose

    URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

    Depositor

    David Botstein

    Inserts

    yZ3EV cassette
    Z3 promoter
    yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPSmOrange-N1

    Plasmid

    #31898

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFBOT563

    Plasmid

    #40864

    Depositor

    Harold Gainer

    Article

    Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

    Insert

    Oxytocin promoter
    

    Use

    AAV

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    mouse oxytocin promoter

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCRISPaint-2A-TurboGFP-PEST

    Plasmid

    #67180

    Purpose

    CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    T2A-TurboGFP-PEST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAVS1-shRNA

    Plasmid

    #82697

    Purpose

    For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    hPGK, U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMSCVhygro-Cre

    Plasmid

    #34565

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    March 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-nuclear-EKAR4

    Plasmid

    #174439

    Purpose

    Nuclear-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    nuclear-EKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP Tubulin K40R

    Plasmid

    #105303

    Purpose

    To study effects of acetylation-null tubulin

    Depositor

    Kenneth Yamada

    Article

    Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K40R

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. hPDI1_18-508_WT_pFLAG-CMV1

    Plasmid

    #31382

    Depositor

    David Ron

    Article

    Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

    Insert

    hPDI1 (P4HB Human)
    

    Use

    Tags

    FLAG M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. p11L1w

    Plasmid

    #37330

    Depositor

    John Schiller

    Article

    Mossadegh et al Virology. 2004 Aug 15;326(1):57-66.

    Insert

    HPV11 L1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP Tubulin K40Q

    Plasmid

    #105302

    Purpose

    To study effects of acetyl-mimetic (hyper-acetylated) tubulin

    Depositor

    Kenneth Yamada

    Article

    Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K40Q

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHsp70-Cas9

    Plasmid

    #45945

    Purpose

    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Expression

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Split 'n coexpress CUL3-RBX1

    Plasmid

    #52293

    Purpose

    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Inserts

    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    

    Use

    Tags

    GST, IRES, and TEV

    Expression

    Bacterial

    Mutation

    contains aa 1-309, contains aa 310-768, and conta…

    Promoter

    IRES and tac

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

45. CF-Ins54p

    Plasmid

    #20155

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-Inp54p (INP54 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Stop codon at position 331.

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52544

    Purpose

    Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AICSDP-50: NPM1-mEGFP

    Plasmid

    #109122

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1

    Depositor

    Allen Institute for Cell Science

    Insert

    NPM1 Homology Arms with linker-mEGFP (NPM1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3-ER-GCaMP3

    Plasmid

    #64854

    Purpose

    Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-GCaMP3
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pT7-VP7SA11

    Plasmid

    #89167

    Purpose

    Express Rotavirus SA11 VP7 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP7
    

    Use

    Tags

    *see below

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge