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4,146 results

Showing: 601 - 630 of 4146 results

  4. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Expression
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Promoter
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

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  6. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  7. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  15. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-82990

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  25. mEGFP-NbALFA

    Plasmid
    #159986
    Purpose
    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mEGFP-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mEGFP and NbALFA
    Promoter
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159986-plasmid-map-sequence-id-314744

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  27. pLVX-puro-cOVA-IRES-BFP

    Plasmid
    #135074
    Purpose
    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.
    Depositor
    Maria Castro
    Insert
    cOVA-IRES-BFP (OVAL Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    135074-plasmid-map-sequence-id-266791

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  28. pTight-9-124-BclxL

    Plasmid
    #60857
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter
    Depositor
    Andrew Yoo
    Article
    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1
    Insert
    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTight
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60857-plasmid-map-sequence-id-195309

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Showing: 601 - 630 of 4146 results