19,210 results
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pET_RGG-RGG
Plasmid#124941PurposeExpresion of RGG-RGGDepositorInsertRGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-1
Plasmid#36409DepositorTypeEmpty backboneUseFluorescent protein cassette for sub-cloningTagsmEGFPExpressionMutationPromoterno promoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
CNR1-Tango
Plasmid#66254PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCNR1 (CNR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dead-Laconic/pcDNA3.1(-)
Plasmid#118627PurposeTo express in mammalian cells a lactate FRET sensor negative control.DepositorInsertDead-Laconic
UseTagsExpressionMammalianMutationChange Histidine 151 to Aspartic Acid of Escheric…PromoterCMV PromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-yellow pre-eGRASP(p30)
Plasmid#111587PurposeAn AAV vector that expresses yellow pre-eGRASP(p30) under the CaMKIIa promoter.DepositorInsertyellow pre-eGRASP(p30)
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KB
Plasmid#139310PurposeRecruitable PI4KBDepositorInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
UseTags10xHis-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT2dR3-proB
Plasmid#122595PurposeBE PACE selection, express T7 RNA polymerase with degron-GTCCA targetDepositorInsertT7 RNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_220d
Plasmid#91114PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRIS
Plasmid#102584PurposeRegulation of protein localization (near-infrared-blue-light-inducible shuttle)DepositorInsertBphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
Plasmid#162381PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterCAGAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAPKAPK2
Plasmid#23426DepositorInsertMAPKAPK2 (MAPKAPK2 Human)
UseGateway donor vectorTagsExpressionMutationP18APromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mNeonGreen
Plasmid#99131PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mNeonGreenDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR/pTER+ (430-1)
Plasmid#17453PurposeGateway entry vector for shRNA expression under H1/TO promoterDepositorTypeEmpty backboneUseRNAi; Entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ hnRNPK myc HIS
Plasmid#71662PurposeMammalian expression of hnRNPKDepositorInserthnRNPK (HNRNPK Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NUP50-N-10
Plasmid#55111PurposeLocalization: Nuclear Pore, Excitation: 587, Emission: 610DepositorInsertNUP50 (NUP50 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNAHygro(+)LAMP2B
Plasmid#86029PurposeExpresses LAMP2B in Mammalian cellsDepositorInsertLysosome-associated membrane protein 2b (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-mCherry
Plasmid#113089PurposeGeneral donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgA1/κ
Plasmid#61888PurposeExpresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)DepositorInsertsHER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP
Plasmid#113888PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
iRFP-P4M-SidM
Plasmid#51470PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
UseTagsiRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
hRAP-pEZT-BM
Plasmid#177986PurposeExpression human RAP containing a C-terminal 6xHis tag in mammalian cellsDepositorInsertLDL receptor related protein associated protein 1 (LRPAP1 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
Plasmid#65418PurposeAAV packaging vector for CamKIIa driven KOR DREADD expressionDepositorInsertCaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
UseAAVTagsHA and mCitrineExpressionMutationPromoterCaMKIIaAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGG-B1
Plasmid#78504PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-HDAC3
Plasmid#63676PurposeMammalian expression of Flag tagged HDAC3DepositorInsertHDAC3 (Hdac3 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX
Plasmid#102787PurposeExpresses the Reverse Transcriptase geneDepositorInsertreverse transcription xenopolymerase
UseTagsExpressionBacterialMutationPromoterT7 promoter + lac operatorAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-H1.5
Plasmid#32898DepositorInsertHistone H1.5 (H1-5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-1.1 M
Plasmid#18722DepositorInsertKusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pp-mCAT1
Plasmid#83948PurposeThis plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectorsDepositorInsertmCAT1 (Slc7a1 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171594PurposeStrong human ACE2 abundance from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
lacI-PT5-gusA-pBAV1k
Plasmid#30501DepositorInsertbeta-glucuronidase (gusA Escherichia coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-human NPM1 wt
Plasmid#160692PurposeMammalian expression of FLAG-tagged NPM1DepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCS2+ YFP-Smo
Plasmid#41086DepositorInsertsmoothened homolog (Smo Mouse)
UseTagsYFPExpressionMammalianMutationYFP inserted after Smo signal sequencePromoterAvailable sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mCAMK-RFP
Plasmid#22908DepositorInsertmouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 human cofilin S3E
Plasmid#50861Purposeexpresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cellsDepositorInsertcofilin 1 S3E (CFL1 Human)
UseTagsEGFPExpressionMammalianMutationS3E psuedo-phosporylated, non-activatablePromoterCMVAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Calnexin-N-14
Plasmid#54021PurposeLocalization: Endoplasmic Reticulum, Excitation: 487, Emission: 509DepositorInsertCalnexin (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-FLuc
Plasmid#83281Purposerecombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)DepositorInsertFirefly Luciferase
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
SBE2-Luc
Plasmid#16500DepositorInsertSmad binding element
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
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tetO-Hand2
Plasmid#46028DepositorInsertHand2 (Hand2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDMD-puro
Plasmid#88895Purpose28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin
Plasmid#134662PurposeExpresses EGFP-tagged plasma membrane markerDepositorInsertvesicle-associated membrane protein 5 (VAMP5 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSG323
Plasmid#115594Purposesurface-displayed NullDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163
Plasmid#127192PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
IGI-P0492 pHR-dCas9-NLS-VPR-mCherry
Plasmid#102245PurposeLentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.DepositorInsertdCas9-VPR-mCherry
UseCRISPR and LentiviralTagsNLS-VPR-mCherryExpressionMammalianMutationPromoterCAGAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS
Plasmid#111830PurposeVinculin tension sensor in lentiviral expression vectorDepositorInsertVinculinTS (VCL Chicken)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CXCR2-Tango
Plasmid#66260PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCXCR2 (CXCR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only
