162,536 results

1. pAAV hPGRN

   Plasmid

   #213682

   Purpose

   Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

   Depositor

   Thomas Kukar

   Article

   Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

   Insert

   Human Progranulin (hPGRN) (GRN Human)
   

   Use

   AAV

   Tags

   Twin-Strep tag and V5 tag (after signal peptide)

   Expression

   Mutation

   Promoter

   cytomegalovirus enhancer/chicken β-actin promoter

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ssFv_lenti_mutATMSPARK2

   Plasmid

   #195250

   Purpose

   express Thr to Ala mutant ATMSPARK2 in a lenti vector

   Depositor

   Xiaokun Shu

   Article

   Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

   Insert

   Thr to Ala mutant ATMSPARK2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ssfv

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PAX6 donor plasmid

   Plasmid

   #105239

   Purpose

   Knock in H2B-GFP into the human PAX6 locus

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

   Insert

   PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSBbi G418 Hu CD19

   Plasmid

   #183247

   Purpose

   Expression of human CD19 antigen in a Sleeping Beauty transposon vector

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   CD19 (CD19 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.2 TDP-43 5F-L YFP

   Plasmid

   #84914

   Purpose

   Mammalian expresion of TDP-43 5F-L YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   F147L, F149L, F194L, F229L, F231L

   Promoter

   CMV

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CMV-Sa-SauriCas9

   Plasmid

   #135967

   Purpose

   Expresses Sa-SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-DIO-mCherry

   Plasmid

   #50462

   Purpose

   Double floxed mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSFFV_mNG3K(1-10)

   Plasmid

   #157993

   Purpose

   Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

   Depositor

   Bo Huang

   Article

   Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

   Insert

   pSFFV_mNG3K(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G27D, D42E, M128I, K153M, T170I

   Promoter

   SFFV

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-pDisplay-Halo-T2A-EGFP

   Plasmid

   #223498

   Purpose

   Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

   Depositor

   James Frank

   Insert

   HaloTag and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HDM_VSV_G

    Plasmid

    #204156

    Purpose

    VSV-G expression plasmid

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    VSV-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLEX307-FURIN-G418

    Plasmid

    #158462

    Purpose

    Constitutive lentiviral expression of human FURIN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    FURIN (FURIN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO-p53-shRNA-941

    Plasmid

    #25637

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-941 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid

    #74315

    Purpose

    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

    Depositor

    Stephen Smith, Martin Meyer

    Article

    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

    Inserts

    zebrafish PSD95
    DsRedExpress
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Y599H and I793S (please see depositor comment bel…

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. Sa-SlugCas9

    Plasmid

    #163797

    Purpose

    Expresses Sa-SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HLTV-hTRIM21

    Plasmid

    #104973

    Purpose

    Purification of His-Lipoyl-hTRIM21 protein from bacteria

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Use

    Tags

    His-Lipoyl

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEFIRES NQO1 WT

    Plasmid

    #61735

    Purpose

    Expresses WT human NQO1

    Depositor

    Yosef Shaul

    Article

    Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    EF1a

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 HA-YY1

    Plasmid

    #104395

    Purpose

    HA-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1 (YY1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-DN-hCUL5-FLAG

    Plasmid

    #15823

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N441

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ampkar

    Plasmid

    #35097

    Purpose

    A fluorescent reporter of AMPK activity and cellular energy stress

    Depositor

    Lewis Cantley

    Article

    Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

    Insert

    ampkar
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    na

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJ23101

    Plasmid

    #70252

    Purpose

    Expresses YFP from J23101 promoter and CFP from standard J23101 promoter

    Depositor

    Jim Haseloff

    Article

    Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

    Insert

    promoter J23101-RBS B0034-EYFP-B0015
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF5/FRT/DEST-3xHA/mGli3/Flag

    Plasmid

    #51246

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

23. TFORF1479

    Plasmid

    #143504

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP63 (TP63 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEF5B-FRT-GFP-αTAT1

    Plasmid

    #27099

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
    

    Use

    Tags

    GFP, S-tag, and TEV site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. qTAG-N-Blast-ultraID

    Plasmid

    #207682

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pLVX-EF1a-tetOn-IRES-G418 (EtO)

    Plasmid

    #84776

    Purpose

    Second generation lenti tetracycline operator (on)

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Insert

    TET OPERATOR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTIGHT

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF2258

    Plasmid

    #142927

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX11 (SOX11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

    Plasmid

    #140086

    Purpose

    Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    LbCpf1 (LbCas12a) crRNA backbone
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PR_GEF YFP (2XPDZ-YFP-LARG DH)

    Plasmid

    #80408

    Purpose

    Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

    Depositor

    Michael Glotzer

    Article

    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

    Insert

    2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmVen-RPA70-IRES-RPA32-IRES-RPA14

    Plasmid

    #211431

    Purpose

    mammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14

    Depositor

    Helmut Pospiech

    Insert

    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    

    Use

    Tags

    mVenus (on RPA70)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 4xmts-mNeonGreen

    Plasmid

    #98876

    Purpose

    In vivo visualization of mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    4xmts
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMXs-C3orf17-gfp

    Plasmid

    #70265

    Purpose

    Expresses C3orf17 in mammalian cells with GFP reporter.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    C3orf17 (NEPRO Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pCAG/ATP11C-HA

    Plasmid

    #209220

    Purpose

    Mammalian expression of ATP11C

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP11C (ATP11C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti6.3/EV

    Plasmid

    #120848

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCherry-Kinesin11-N-18

    Plasmid

    #55067

    Purpose

    Localization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Kinesin11 (KIF11 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

    Plasmid

    #169097

    Purpose

    Cas9 and Citrine expression in transfected cells

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    Cas9-2A-Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHEE401

    Plasmid

    #71286

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGWT35S-mCherry

    Plasmid

    #182582

    Purpose

    Transient expression of mCherry in plant cell (Cytosol)

    Depositor

    Yutaka Kodama

    Article

    Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. flex-ChrimsonR-EYFP-Kv

    Plasmid

    #135319

    Purpose

    Cre-dependent expression of soma-targeted ChrimsonR

    Depositor

    Hongkui Zeng

    Article

    Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    synapsin

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-hsyn-GRAB_5-HT1.0

    Plasmid

    #140552

    Purpose

    Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    5-HT sensor GRAB_5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLenti Lifeact-mRuby2 PuroR

    Plasmid

    #84386

    Purpose

    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. 3541 pMIG Bcl-XL

    Plasmid

    #8790

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLVX-CMV-Galpha-13 ONE-GO

    Plasmid

    #189740

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-13 ONE-GO
    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L342M (present in YFP but no functional consequen…

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. TFORF3041

    Plasmid

    #144517

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX15 (SOX15 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. APOBEC3G_pLX307

    Plasmid

    #98345

    Purpose

    Lentiviral expression of APOBEC3G

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    APOBEC3G (APOBEC3G Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pE-SUMO CRYGD

    Plasmid

    #80755

    Purpose

    Expression of human gamma D-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGD (CRYGD Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pDONR223-PTK2

    Plasmid

    #23902

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pC035 - dLwCas13a-msfGFP

    Plasmid

    #91925

    Purpose

    Expresses catalytically inactive LwCas13a for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS-3xHA and NLS-msfGFP

    Expression

    Mammalian

    Mutation

    R488A and R1060A

    Promoter

    EF-1a

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. AAT-PB-CD2APtk

    Plasmid

    #86004

    Purpose

    homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Insert

    DsRedEx_T2A_puro_dTK
    

    Use

    Piggybac exciseable

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge