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179,521 results

Showing: 8841 - 8880 of 179521 results

  3. pX551-CMV-SpCas9

    Plasmid
    #107024
    Purpose
    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107024-plasmid-map-sequence-id-208524

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  4. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid
    #100834
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    CamKII
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100834-plasmid-map-sequence-id-259370

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  5. pAAV.CMV.LacZ.bGH

    Plasmid
    #105531
    Purpose
    AAV expression of LacZ from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV8
    Insert
    LacZ
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105531-plasmid-map-sequence-id-202365

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  7. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103869-plasmid-map-sequence-id-199177

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  9. VN-Tau (P301L)

    Plasmid
    #87634
    Purpose
    expresses hTau fused to the N-terminal part of Venus
    Depositor
    Tiago Outeiro
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPT (MAPT Human)
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Mutation P301L
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87634-plasmid-map-sequence-id-176933

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  10. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid
    #99315
    Purpose
    Negative control for luciferase validation vectors
    Depositor
    Alexander Stark
    Publication
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    neutral region; chr3: 55198740-55200041
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99315-plasmid-map-sequence-id-193174

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  12. pJL 89

    Plasmid
    #98558
    Purpose
    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.
    Depositor
    John Lindbo
    Publication
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Type
    Empty backbone
    Expression
    Plant
    Promoter
    Enh 35S
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98558-plasmid-map-sequence-id-192654

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  17. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Publication
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89898-plasmid-map-sequence-id-178222

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  18. pDONR223_JAK2_WT

    Plasmid
    #81756
    Purpose
    Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.
    Depositor
    Jesse Boehm, William Hahn, David Root
    Publication
    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi
    Insert
    JAK2 (JAK2 Human)
    Use
    Gateway Cloning
    Mutation
    V617F
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    81756-plasmid-map-sequence-id-168863

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  23. AICSDP-23:TJP1-mEGFP

    Plasmid
    #87429
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    87429-plasmid-map-sequence-id-174170

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  24. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Publication
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86883-plasmid-map-sequence-id-173280

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  26. pDisplay-HRP-KDEL

    Plasmid
    #85582
    Purpose
    Expresses HRP-KDEL in mammalian cells
    Depositor
    Alice Ting
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HRP-KDEL
    Tags
    V5
    Expression
    Mammalian
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85582-plasmid-map-sequence-id-165896

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  33. PA-Cdc42

    Plasmid
    #75263
    Purpose
    Light-activated Cdc42
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cell Division Cycle 42 (CDC42 Human)
    Tags
    6xHIs, LOV2, and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    F56W
    Promoter
    CMV
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75263-plasmid-map-sequence-id-153951

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Showing: 8841 - 8880 of 179521 results