164,127 results

1. pLENTI_RPS10

   Plasmid

   #127129

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   RPS10 (RPS10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   K24R- please see depositor comments

   Promoter

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-Tet-Cas9-BFP-PGK-Blast

   Plasmid

   #196720

   Purpose

   Tet-inducible expression of Cas9-T2A-TagBFP

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-TfR-OVA

   Plasmid

   #64600

   Purpose

   Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

   Depositor

   Sandra Diebold, Martin Zenke

   Article

   Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

   Insert

   Ovalbumin
   

   Use

   Tags

   Transmembrane domain of Transferrin Receptor (TfR…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG mito-mEos2

   Plasmid

   #168515

   Purpose

   Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

   Insert

   mito-mEos2
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

   Plasmid

   #164510

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Has Service

   AAV9

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Synthetic Construct

   Promoter

   Synapsin

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EYFP-ER-0

   Plasmid

   #56589

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Insert

   ER (CALR Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. H3K9me3 biosensor

   Plasmid

   #120802

   Purpose

   FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOSIP-KP (KanR, Phi80)

   Plasmid

   #45986

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2848

   Plasmid

   #144249

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF21 (TCF21 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-hPGK

    Plasmid

    #82579

    Purpose

    5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    Human PGK promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. M7 pdnPKA-GFP

    Plasmid

    #16716

    Depositor

    Randall Moon

    Article

    Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

    Insert

    dnPKA (Prkar1a Mouse)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Dominant negative. Mutations at cAMP binding site…

    Promoter

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMC-6-ZmBbm-P2A-ZmWus2-9

    Plasmid

    #197750

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmBbm-P2A-ZmWus2 bicistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid

    #207792

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-Tet1-CD

    Plasmid

    #83340

    Purpose

    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    U6, CBH

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-PTEN

    Plasmid

    #116780

    Purpose

    Lentiviral expression of PTEN

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBOB-synP-HTB

    Plasmid

    #30195

    Depositor

    Edward Callaway, Liqun Luo

    Article

    Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

    Insert

    histone-GFP-2A-TVA receptor-2A-rabies glycoprotein
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB Neo* PB

    Plasmid

    #138413

    Purpose

    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Mouse Targeting; Piggybac

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX_306

    Plasmid

    #41391

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSKI015

    Plasmid

    #11570

    Depositor

    Detlef Weigel

    Article

    Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

    Insert

    CaMV 35S enhancers
    

    Use

    Activation tagging

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P4-TadCBEd

    Plasmid

    #225143

    Purpose

    Packaging plasmid for TadCBEd in v3b PE-eVLP architecture

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-TadCBEd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF1034

    Plasmid

    #143745

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    WT1 (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL3 RARE-d2EGFP

    Plasmid

    #183055

    Purpose

    Destabilized EGFP fluorescent reporter for Retinoic Acid activity

    Depositor

    Chaya Kalcheim

    Article

    Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

    Insert

    d2EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    RARE, SV40

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pICH-chl-roGFP2-synORP1

    Plasmid

    #191701

    Purpose

    Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-roGFP2-synORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICH-nuc-roGFP2-synORP1

    Plasmid

    #191759

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-synORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenLox_U6 p53

    Plasmid

    #59360

    Purpose

    Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

    Depositor

    Peter Scheiffele

    Article

    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

    Inserts

    shRNA p53 (mouse) (Trp53 Mouse)
    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and mouse U6

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ECFP HO

    Plasmid

    #207413

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ECFP CD

    Plasmid

    #207414

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA-HIF1alpha P402A/P564A-pBabe-puro

    Plasmid

    #19005

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Promoter

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA3 HaloTag-V5-TurboID

    Plasmid

    #226474

    Purpose

    Expresses fusion of HaloTag and TurboID in mammalian cells

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Cell Chem Biol. 2022 Dec 15;29(12):1739-

    Insert

    TurboID
    

    Use

    Tags

    HaloTag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP-C1-Ataxin3Q84

    Plasmid

    #22123

    Depositor

    Henry Paulson

    Article

    Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEGFP-C1-TBP

    Plasmid

    #26674

    Depositor

    Sui Huang

    Article

    Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

    Insert

    TBP (TBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS314-URA3

    Plasmid

    #11004

    Depositor

    Francisco Malagon

    Insert

    URA3 (URA3 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Promoter

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PRKAG3 gRNA (BRDN0001145578)

    Plasmid

    #77295

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PRKAG3 gRNA (BRDN0001147132)

    Plasmid

    #77296

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PRKAG3 gRNA (BRDN0001146704)

    Plasmid

    #77294

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLVX-NPM1wt-IRES-mCherry

    Plasmid

    #131824

    Purpose

    Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMIG-IRF4

    Plasmid

    #58987

    Purpose

    MSCV based expression vector - co-expresses GFP and murine IRF4

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Insert

    Mouse IRF4 (Irf4 Mouse)
    

    Use

    Retroviral

    Tags

    Kozak sequence added

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge