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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,735 results for: pcDNA

Showing: 11301 - 11350 of 11735 results

  5. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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  12. pKK-TEV-mTagBFP

    Plasmid
    #105789
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mTagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105789-plasmid-map-sequence-id-203380

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  18. mCerulean-proα2(I)

    Plasmid
    #119836
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119836-plasmid-map-sequence-id-233866

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  23. mVenus-proα1(I)

    Plasmid
    #119845
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119845-plasmid-map-sequence-id-233871

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  31. MPC1-AA-FLAG

    Plasmid
    #228904
    Purpose
    Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with alanine blocking acetylation with a C terminal FLAG tag
    Depositor
    Ghazaleh Ashrafi
    Article
    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do
    Insert
    MPC1 (Mpc1 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K45 and K46 motif sites were both replaced with a…
    Promoter
    CMV
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228904-plasmid-map-sequence-id-460265

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  32. Epac-S-H326

    Plasmid
    #228242
    Purpose
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228242-plasmid-map-sequence-id-454214

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  33. Epac-S-H322

    Plasmid
    #228246
    Purpose
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228246-plasmid-map-sequence-id-451789

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  34. Epac-S-H323

    Plasmid
    #228247
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228247-plasmid-map-sequence-id-451793

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  35. Epac-S-H324

    Plasmid
    #228248
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228248-plasmid-map-sequence-id-451797

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  36. Epac-S-H325

    Plasmid
    #228249
    Purpose
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228249-plasmid-map-sequence-id-451794

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  37. Epac-S-H332

    Plasmid
    #228250
    Purpose
    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228250-plasmid-map-sequence-id-451844

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  48. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid
    #210024
    Purpose
    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Encephalomyocarditis virus internal ribosome entr…
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210024-plasmid-map-sequence-id-417730

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Showing: 11301 - 11350 of 11735 results