8,969 results

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pLenti-CaMKIIa-eArchT 3.0-EYFP

Plasmid

#35513

Purpose

Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArchT 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTALYM4SpMi-05

Plasmid

#47880

Purpose

TALE-mRuby2 against Mus spretus/musculus minor satellite sequence

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

TALE-mRuby2

Use

TALEN

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pK7WGF2::hCas9

Plasmid

#46965

Purpose

Expresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

hCas9

Use

CRISPR; Plant expression

Tags

eGFP

Promoter

35S

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pLVET-tTR-KRAB

Plasmid

#11644

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hEF-1alpha, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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human Alpha-synuclein

Plasmid

#51437

Purpose

human codon optimized Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (SNCA Human)

Use

Cloning

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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IL-2 promoter luciferase

Plasmid

#12194

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-2 promoter (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

Plasmid

#141371

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

C145A, human codon optimized

Promoter

EF1alpha

Available Since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGL2-AN

Plasmid

#20950

Depositor

William Kaelin

Article

Neuman et al Mol Cell Biol. 1994 Oct . 14(10):6607-15

Insert

E2F-1 promoter, genomic fragment (E2F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

wt human E2F-1 promoter sequence

Available Since

Aug. 10, 2009

Availability

Academic Institutions and Nonprofits only

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mRFP1-C1

Plasmid

#54764

Purpose

Localization: C1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pPHT-PKA

Plasmid

#60936

Purpose

Fluorescent FPX sensor for cAMP

Depositor

Anne Marie Quinn

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

PKA FPX Sensor

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p3869 HA-E6-AP C833A

Plasmid

#8649

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C833A; active site Cys mutated to encode Ala

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-hSynapsin1-FLEx-axon-GCaMP6s

Plasmid

#112010

Purpose

For cre-dependent enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV5 and AAV9

Insert

axon-GCaMP6s

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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paGFP

Plasmid

#18697

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation

Promoter

CMV

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3β-FLAG-CBP-LD-HA

Plasmid

#32906

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

C/EBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

L1435A/D1436A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

Plasmid

#86233

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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PGKneotpAlox2

Plasmid

#13444

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

PGKneotpAlox2

Use

Cre/Lox

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Promoter

tac promoter

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRIF Flag

Plasmid

#41550

Depositor

Kate Fitzgerald, Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

Trif (TICAM1 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pCI-neo Flag HAUSP

Plasmid

#16655

Depositor

Bert Vogelstein

Article

Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

Insert

HAUSP (USP7 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIbeta

Plasmid

#21227

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIbeta (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0002.

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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CXCR4-Tango

Plasmid

#66262

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CXCR4 (CXCR4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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AcGFP1-N1

Plasmid

#54705

Purpose

Localization: N1 Cloning Vector, Excitation: 475, Emission: 505

Depositor

Michael Davidson

Type

Empty backbone

Tags

AcGFP1

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-H-Ras_wt

Plasmid

#39503

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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SV40 small + Large T in pENTR1A (w611-7)

Plasmid

#22297

Depositor

Eric Campeau

Insert

SV40 small + Large T antigen

Use

Gateway Cloning

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag DLC1

Plasmid

#24265

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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IKK-2 K44M

Plasmid

#11104

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

K44M, kinase inactive

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro

Plasmid

#141393

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9c

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AAV-flex-ReaChR-citrine

Plasmid

#50955

Purpose

flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

human synapsin promoter

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,969 results