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159,352 results

Showing: 9801 - 9840 of 159352 results

  2. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

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  5. pNJB91

    Plasmid
    #51519
    Purpose
    Gateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51519-plasmid-map-sequence-id-78693

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  17. pNJB80

    Plasmid
    #51518
    Purpose
    Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51518-plasmid-map-sequence-id-78690

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  21. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    P45
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  22. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    krt1-ex1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. TFORF1259

    Plasmid
    #142770
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP53 (TP53 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142770-plasmid-map-sequence-id-395839

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  24. pM2s2AsG

    Plasmid
    #137924
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137924-plasmid-map-sequence-id-331367

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  28. pLenti-EGFP-SnxA

    Plasmid
    #232460
    Purpose
    Lentiviral EGFP lipid sensor for PI(3,5)P2
    Depositor
    Ken-Ichi Takemaru
    Insert
    EGFP-SnxA
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232460-plasmid-map-sequence-id-464306

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  33. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  34. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

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  37. pQE81XN-msYFP

    Plasmid
    #218960
    Purpose
    Expresses Hisx6-Tagged msYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    msYFP
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218960-plasmid-map-sequence-id-446223

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  38. PRS_8-GCaMP8m

    Plasmid
    #210400
    Purpose
    AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons
    Depositor
    Simon Wiegert
    Article
    Wissing et al bioRxiv 2022.01.22.477348
    Insert
    GCaMP8m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRSx8
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    210400-plasmid-map-sequence-id-415628

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Showing: 9801 - 9840 of 159352 results