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We narrowed to 13,951 results for: CRISPR-Cas9

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Showing: 10001 - 10040 of 13951 results

  7. VRER variant

    Plasmid
    #138561
    Purpose
    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRER
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138561-plasmid-map-sequence-id-274783

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  15. pTR(EF)-tRNA(Q)

    Plasmid
    #170517
    Purpose
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(EF)-tRNA(Q)
    Use
    Pcr template vector for amplifying grnacore(ef)-t…
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170517-plasmid-map-sequence-id-334636

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  17. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  18. GE-0010-gRNA2-MpCKB

    Plasmid
    #238528
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta
    Depositor
    Michael Hothorn
    Insert
    MpCKB
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238528-plasmid-map-sequence-id-477293

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  19. GE-0010-gRNA1-MpCKB

    Plasmid
    #238527
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta
    Depositor
    Michael Hothorn
    Insert
    MpCKB
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238527-plasmid-map-sequence-id-477292

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  20. GE-0010-gRNA1-MpCKA

    Plasmid
    #238526
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 alpha
    Depositor
    Michael Hothorn
    Insert
    MpCKA
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238526-plasmid-map-sequence-id-477280

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  21. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  23. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  25. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

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  30. pUC119-gRNA

    Plasmid
    #52255
    Purpose
    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
    Depositor
    Jen Sheen
    Article
    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d
    Insert
    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    Use
    CRISPR; Plant expression
    Promoter
    Arabidopsis U6 polymerase III promoter
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52255-plasmid-map-sequence-id-80847

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  35. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid
    #160621
    Purpose
    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    U6-26:gRNA 4 (pNos): 2.1 aptamer
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160621-plasmid-map-sequence-id-322006

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  40. pTG131

    Plasmid
    #198640
    Purpose
    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    Cas9
    Expression
    Bacterial
    Promoter
    pPhlF
    Available Since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198640-plasmid-map-sequence-id-396878

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Showing: 10001 - 10040 of 13951 results