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Plasmid#24041DepositorInsertRPL25 (RPL25 Budding Yeast)
UseTagsEGFP and PRAExpressionYeastMutationOnly bp 1-135 (aa 1-45) of RPL25PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL-CP
Plasmid#65715PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol1-uas-mAKT-2a-nlstdTomato
Plasmid#67706PurposeTol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomatoDepositorInsertmAKT-2A-nlstdTomato
UseTagsExpressionMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
Plasmid#172195PurposeAAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationtranscription regulatory domain removedPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pattB-nsyb-MKIIK11A::nlsLexADBDo
Plasmid#64726Purposegenerating transgenic TRIC fliesDepositorInsertMKIIK11A::nlsLexADBDo
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-TetR-NLS-VP16
Plasmid#103835PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInserttagBFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEMAX-NLS-P2A_BLAST
Plasmid#135355PurposeExpression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertABEMax-2XNLS
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-dsm
Plasmid#89743PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11DepositorInsertNLS-OptoJNKi dark-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-lsm
Plasmid#89742PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11DepositorInsertNLS-OptoJNKi lit-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationI539E mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-PCNA-19-SV40NLS-4
Plasmid#57154PurposeLocalization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-S5E8-dTom-nlsdTom
Plasmid#135643PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVTagsExpressionMutationNonePromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
Plasmid#85821PurposeiDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertZf Ubi::cMyc oDam_f_GFPoNLS
UseTol2 based vectorTagscMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…ExpressionMutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS ΔNLS 1-513
Plasmid#44984DepositorInsertFUS ΔNLS (1-513aa) (FUS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 1-513PromotertacAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-YNL
Plasmid#65711PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL)DepositorInsert7xTcf, minCMV, 3xNLS, YNL
UseLuciferase; Tol2 systemTagsExpressionMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_2_+nls-eGFP-HA-_+1_BK+
Plasmid#49312PurposeGolden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codonDepositorInsertnls-EGFP-HA
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
Plasmid#67687PurposeUAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassetteDepositorInsertdusp6 (dusp6 Zebrafish)
UseTags6xmyc-NLS-tdTomato-2AExpressionMutationS159A, S196APromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
PHO4NLS-GFP-PRA (109)
Plasmid#24044DepositorInsertPHO4
UseTagsEGFP and PrAExpressionYeastMutationOnly bp 421-588 of PHO4 (NLS)PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-NLS-VP16
Plasmid#103822PurposeSoluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP-VP16 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationVP16: G126T (silent, G42G)PromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
NAB2NLS-GFP-PRA (107)
Plasmid#24043DepositorInsertNAB2
UseTagsEGFP and PrAExpressionYeastMutationOnly bp 601-750 of NAB2 (NLS)PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEBB-GFP-hBEN-deltaNLS3
Plasmid#22158DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGFPExpressionMammalianMutationDeletion of amino acids 883-889PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-NLS-BFP-mEosEM
Plasmid#218934PurposeExpress the nuclear import substrate NLS-BFP-mEosEM.DepositorInsertBFP-mEosEM fusion protein
UseTags6xHis and SV40 nuclear localization sequence (NLS…ExpressionBacterialMutationPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40
Plasmid#233365PurposeCAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transductionDepositorInsertKinprola_PKA-mEGFP-NLS3X
UseAAVTagsmEGFP-NLS3XExpressionMutationPromoterCAGAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
UBQ10pro-nlsABACUS2-400n-NosT
Plasmid#203728PurposeBinary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into ArabidopsisDepositorInsertnlsABACUS2-400n
UseSynthetic BiologyTagsMycExpressionPlantMutationPromoterAtUBQ10pro 1500bpAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NLS-HA (pKW919)
Plasmid#229047PurposeMammalian expression of CasRx-NLS-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3
Plasmid#231568PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertAll-in-one LOOMINA construct
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX2-SOPP3-3xNLS
Plasmid#229074Purposeexpresses APEX2-SOPP3 in the mammalian nucleusDepositorInsertExpress APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
CPH3375_SF_Spt6p-tSH2-1223-1451_NLS
Plasmid#227084PurposeGal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleusDepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsSTREPii-FLAG and SV40 NLS 1ExpressionYeastMutationPromoterGal1-10Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-AGG-NLS-EGFP
Plasmid#214602Purposeexpress EGFP ; in vitro transcribed modified RNA vectorDepositorInsertEGFP
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLS-VP16-NIR-Fb(718)
Plasmid#220745PurposeNLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyTagsNLS-VP16ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-EGFP
Plasmid#220594PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.DepositorInsertEGFP
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-miRFP670
Plasmid#220600PurposeConstitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.DepositorInsertmiRFP670
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mKate2
Plasmid#220599PurposeConstitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.DepositorInsertmKate2
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-AausFP1
Plasmid#220595PurposeConstitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.DepositorInsertAausFP1
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-AL-mCh-Cry2WT-NLS
Plasmid#221924PurposeExpression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP AL (aa 1529-1607) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the autoregulatory loop (AL) (aa 152…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_NLS-Halo-eGFP-BASU
Plasmid#218449Purposeexpressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integrationDepositorInsertNLS-Halo-eGFP-BASU
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-HKGT3_mCherry2-NLS-PEST_Puro
Plasmid#213771PurposeFluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.DepositorInsertHKGT3_mCherry2-NLS-PEST
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)
Plasmid#211861PurposeNuclear-localized E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)
Plasmid#211863PurposeNuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAExpressionMutationC492APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1alpha-AcrIC8-NLS
Plasmid#207395PurposeExpresses AcrIC8 with NLS in mammalian cellsDepositorInsertEF1a-AcrIC8-bGH polyA
UseTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1alpha-AcrIE2-NLS
Plasmid#207396PurposeExpresses AcrIE2 with NLS in mammalian cellsDepositorInsertEF1a-AcrIE2-bGH polyA
UseTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only