164,223 results

1. pQC-CherryOFF-GFP

   Plasmid

   #129101

   Purpose

   Reporter vector

   Depositor

   Fangliang Zhang

   Article

   Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

   Insert

   CherryOFF
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G-> A

   Promoter

   CMV

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6P-1-GST-CD(Cbx7)

   Plasmid

   #82525

   Purpose

   Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 7 (CBX7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   CD(Cbx7) ; amino acids 1–62

   Promoter

   Available Since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTHSSd_14

   Plasmid

   #59952

   Purpose

   Triple split (at 67, 601-SZ)

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   Three-fragment T7* RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p7140 pHAGE-P-CMVt-N-HA-GAW-EME1

   Plasmid

   #100150

   Purpose

   Expresses EME1

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   EME1 (EME1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEEV2/11

   Plasmid

   #166888

   Purpose

   Helper/packaging plasmid for production of AAV2/11 pseudotype vector

   Depositor

   Tadahito Kanda, Seiichiro Mori

   Article

   Mori et al Virology. 2004 Dec 20;330(2):375-83. doi

   Insert

   AAV2 rep/AAV11 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   AAV2 p5

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

   Plasmid

   #203908

   Purpose

   Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   Gal4UAS_dGFP_PGK_iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   5xUAS and PGK

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXG237

   Plasmid

   #141281

   Purpose

   ATF4 translational reporter

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   uORF1/2 of ATF4 followed by mApple
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. plentiCMV-sct-p5-b2m-HLA-A0101

   Plasmid

   #196512

   Purpose

   Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a CMV peptide, b2m and HLA A0101
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-mKeima-LGALS3

    Plasmid

    #175780

    Purpose

    Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMYC-GFP

    Plasmid

    #42142

    Depositor

    Olufunmilayo Olopade

    Article

    Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

    Insert

    c-Myc (MYC Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. hPGK_V5-APEX2-GFP

    Plasmid

    #105285

    Purpose

    drives expression of V5-NES-APEX2-GFP from human PGK promotor

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    V5-APEX2-NES
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK promoter

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTU-DO-HIS

    Plasmid

    #160005

    Purpose

    Low-copy expression vector for K. marxianus, histidine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CALM-pmCherryN1

    Plasmid

    #27691

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    CALM (Picalm Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EF1a_ESRRG_P2A_Hygro_Barcode

    Plasmid

    #120435

    Purpose

    Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ESRRG (ESRRG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. XE118 CAMKII-T286D-CS2+

    Plasmid

    #16736

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-T286D (Camk2a Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    threonine 286 mutated to aspartate

    Promoter

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pdest METTL1-WT Neo

    Plasmid

    #223058

    Purpose

    METTL1 gene expression in mammalian cells

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    METTL1 (METTL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPAmCherry-a-tubulin

    Plasmid

    #31930

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    a-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-C-Myc-DDK-IRES-Puro LARP7

    Plasmid

    #200725

    Purpose

    Lentiviral expression of Flag-Tagged human LARP7

    Depositor

    Claudio Alarcón

    Insert

    LARP7 (LARP7 Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sg2.02 16x(MS2) MUC4.1

    Plasmid

    #101154

    Purpose

    gRNA with 16 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HIR WT

    Plasmid

    #24049

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-Ef1a-Con/Fon-oScarlet

    Plasmid

    #137136

    Purpose

    Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-BRAF-V600E

    Plasmid

    #116204

    Purpose

    Lentiviral expression of BRAF V600E

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V600E

    Promoter

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    SP6

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pD649-HAsp-AGER-COMP5AP-AviTag-9xHis

    Plasmid

    #157325

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    AGER (AGER Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-C1-CAMKIIalpha-T286A

    Plasmid

    #21220

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIalpha-T286A (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T286A (Lab plasmid KS 0003).

    Promoter

    Available Since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pUltra-puro-RTTA3

    Plasmid

    #58750

    Purpose

    silence doxycyclin inducible pUltra-dox

    Depositor

    Kitai Kim, Yildirim Dogan

    Insert

    Reverse TET-Transactivator 3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    loxP5171

    Promoter

    Ubiquitin C promoter

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. dRED

    Plasmid

    #139836

    Purpose

    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

    Depositor

    Karim Mekhail

    Article

    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

    Insert

    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RNase H1 protein contains D210N mutation. dCas9 …

    Promoter

    tet-inducible CMV

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CaMKIIa-rsChRmine-oScarlet-WPRE

    Plasmid

    #183522

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    rsChRmine-oScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I146M/G174S

    Promoter

    CaMKIIa

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6/V5-DEST-NCAPH

    Plasmid

    #31214

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NCAPH (NCAPH Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pScaf-3024.1

    Plasmid

    #111404

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 3024 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-3024
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMK290 (mAID-mClover-Hygro)

    Plasmid

    #72828

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Fuw-dCas9-Dnmt3a

    Plasmid

    #84476

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-Flag-gp130dimer

    Plasmid

    #174394

    Purpose

    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain with leucine zipper & C-C linker
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAID1.2-EF1a-CGFP-mAID

    Plasmid

    #140608

    Purpose

    Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. VPX-mRNA

    Plasmid

    #227679

    Purpose

    plasmid for VPX mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    VPX (HIV2gp3 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Cdk5-HA

    Plasmid

    #1872

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk5 (CDK5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMTU-DO-LEU

    Plasmid

    #160006

    Purpose

    Low-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCB6-EnvA

    Plasmid

    #74420

    Purpose

    Mammalian expression of the subgroup A avian leukosis-sarcoma envelope glycoprotein (EnvA)

    Depositor

    Judith White

    Article

    Gilbert et al J Virol. 1994 Sep;68(9):5623-8.

    Insert

    Env from ASLV-A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

40. LeGO-iG2

    Plasmid

    #27341

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, IRES-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge