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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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163,570 results

Showing: 1041 - 1080 of 163570 results

  1. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

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  4. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and nuclear localization sequence
    Use
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98998-plasmid-map-sequence-id-190366

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  5. pBsVchCAST-pyrE

    Plasmid
    #203813
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
    Depositor
    Sheng Yang
    Article
    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203813-plasmid-map-sequence-id-403827

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  13. mCerulean-Nucleus-12

    Plasmid
    #54556
    Purpose
    Localization: Nucleus, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mCerulean-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. TFORF0266

    Plasmid
    #142578
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF4 (TCF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142578-plasmid-map-sequence-id-396000

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  17. SENP2M497A

    Plasmid
    #61088
    Purpose
    cleave the SUMO off when use PETSUMO vector for express fusion protein
    Depositor
    Cynthia Wolberger
    Article
    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033
    Insert
    SENP2M497A catalytic domain (SENP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M497A; Catalytic domain is residues 364-589
    Promoter
    T7
    Available Since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pDONR221-CLN3_STOP

    Plasmid
    #161034
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    CLN3 (CLN3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161034-plasmid-map-sequence-id-386448

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  34. RAD21-HaloTag Vector

    Plasmid
    #238694
    Purpose
    Express RAD21-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    RAD21 (RAD21 Human)
    Use
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238694-plasmid-map-sequence-id-479890

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  35. IL13Ra1-HaloTag BiBRET Vector

    Plasmid
    #238673
    Purpose
    Express IL13Ra1-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    IL13Ra1 (IL13RA1 Human)
    Use
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238673-plasmid-map-sequence-id-482865

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  40. pSIN4-BCAT1

    Plasmid
    #240021
    Purpose
    Expresses human BCAT1 fused to 3X Flag in mammalian cells
    Depositor
    Rui Su
    Insert
    BCAT1
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mutation
    Promoter
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    240021-plasmid-map-sequence-id-479100

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Showing: 1041 - 1080 of 163570 results