166,041 results

1. pTR-374 tNGFR-P2A-B2M

   Plasmid

   #186068

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   B2M (B2M Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbS8k-RFP_R43W_E93G

   Plasmid

   #109406

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   R43W and E93G repA mutations to increase copy num…

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRetroX-TRE3G_NT-GSDMD_I105N_

   Plasmid

   #201174

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Mutation

   aa1-276 only, I105N

   Promoter

   TRE3G

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2RY8-Tango

   Plasmid

   #66477

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   P2RY8 (P2RY8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

   Plasmid

   #176135

   Purpose

   Heterodimerization with FRB (rapamycin), Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Entry vector

   Mutation

   See Depositor Comments Below

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN-LacI

   Plasmid

   #103814

   Purpose

   BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
   

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRESpuro3+mRFP-Utr_CH

   Plasmid

   #64358

   Purpose

   bicistronic expression of Utrophin-mRFP and Puro resistance

   Depositor

   Matthieu Piel

   Insert

   Utrophin-mRFP (UTRN Human)
   

   Tags

   IRES-Puro and mRFP

   Expression

   Mammalian

   Mutation

   Q236R; aa1-261 Utrophin present

   Promoter

   CMV

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC35G2

   Plasmid

   #132144

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35G2 (TMEM22 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR221_SLC35G6

   Plasmid

   #132096

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35G6 (SLC35G6 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. R777-E180 Hs.PIK3R5-nostop

    Plasmid

    #70464

    Purpose

    Gateway ORF clone of human PIK3R5 [NM_014308.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R5 (PIK3R5 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

    Plasmid

    #68280

    Purpose

    SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Mkk2DS NLS mCherry (MKK2 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    RPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-10-3xFLAG-LGP2-K651E

    Plasmid

    #58685

    Purpose

    Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

    Insert

    LGP2-K651E (DHX58 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    mutation to CTD, K651E

    Promoter

    CMV

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pPB-EF1α-IB-H2B-HaloTag

    Plasmid

    #247449

    Purpose

    Expresses H2B fused with HaloTag.

    Depositor

    Kazuhiro Maeshima

    Article

    Otsuka et al Chromosoma. 2024 Apr;133(2):135-148. doi

    Insert

    H2B (H2BC11) (H2BC11 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Nov. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE30-HyPerFLEX

    Plasmid

    #217569

    Purpose

    Bacterial expression of HyPerFLEX

    Depositor

    Vsevolod Belousov

    Article

    Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

    Insert

    HyPerFLEX
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Oct. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. RARe-RFP-PGK-H2BGFP

    Plasmid

    #241863

    Purpose

    retinoic acid response element (RARe)-driven fluorescent reporter

    Depositor

    Elaine Fuchs

    Article

    Tierney et al Science. 2024 Mar 8;383(6687):eadi7342.

    Insert

    RARe-RFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965)

    Plasmid

    #242652

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-ABE8.8m-SpG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.8 mutations in TadA, SpG mutations in SpCas9…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-ABE8.20m-eVRQR-P2A-EGFP (LLH569)

    Plasmid

    #242656

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.20 A-to-G base editor with neVRQR(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-ABE8.20m-VRQR-S55R-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA, eVRQR mutations in SpC…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSSBIO32_dCas9_RPA1786

    Plasmid

    #240489

    Purpose

    CRISPRi-mediated Knockdown of RPA1786

    Depositor

    Rajib Saha

    Insert

    Cas9 (cas9 Streptococcus pyogenes)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Plac

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. LgBiT-KRAS(G12D) Vector

    Plasmid

    #237033

    Purpose

    Express LgBiT-KRAS(G12D) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    KRAS (KRAS Human)
    

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. hCHMP4B-HaloTag Vector

    Plasmid

    #236971

    Purpose

    Express hCHMP4B-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    hCHMP4B (CHMP4B Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pFUW PRKN-P2A-PuroR

    Plasmid

    #239571

    Purpose

    Overexpression of human PARK2

    Depositor

    Tim Ahfeldt, Joel Blanchard

    Article

    et al iScience 28 (2025)

    Insert

    PRKN (PRKN Human)
    

    Use

    Lentiviral

    Promoter

    UbC

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBAD-ApplePy1Lowest

    Plasmid

    #239057

    Purpose

    Bacterial expression of lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    ApplePy1Lowest
    

    Expression

    Bacterial

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. pTW335p3

    Plasmid

    #237329

    Purpose

    expresses Z. mays RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Inserts

    Z. mays RbcL
    Z. mays RbcS-6xHis
    

    Tags

    6xHis and None

    Expression

    Bacterial

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP-TDP-43 (5FL)

    Plasmid

    #235491

    Purpose

    Expression of human RNA-binding deficient TDP-43-EGFP

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F124L, F127L, F147L, F149L, F152L

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pCWU50

    Plasmid

    #232318

    Purpose

    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

    Depositor

    Chenggang Wu

    Insert

    Pfdx-E
    

    Expression

    Bacterial

    Promoter

    fdx

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

    Plasmid

    #225588

    Purpose

    Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2(S54L+T127A)-T2A-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLVX MLKL(S345D)-2A-mCherry-puro

    Plasmid

    #231975

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Mutation

    MLKL S345D

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pHAGE-EF1a-HSF1-GFP

    Plasmid

    #227820

    Purpose

    Expresses HSF1 fused to GFP from EF1a promoter

    Depositor

    Jeffrey Gerst

    Article

    Pataki et al bioRxiv 2024.10.05.616786

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET24a_SMOX

    Plasmid

    #186345

    Purpose

    C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

    Insert

    Spermine oxidase (SMOX Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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31. pT7-AGG-SpCas9

    Plasmid

    #214603

    Purpose

    encode SpCas9; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    SpCas9
    

    Use

    CRISPR and Unspecified

    Promoter

    T7

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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32. pNK5664

    Plasmid

    #219747

    Purpose

    DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - nnLuz_v4 - tSV40
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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33. pMx/ATP8B3-HA

    Plasmid

    #209228

    Purpose

    Mammalian expression of ATP8B3

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B3 (ATP8B3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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34. pCAG/ATP8B3-HA

    Plasmid

    #209211

    Purpose

    Mammalian expression of ATP8B3

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP8B3 (ATP8B3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUG34-NES-EGFP-cPHx3

    Plasmid

    #183669

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Frog, Human)
    

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEntr1a-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176134

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR221_SLC25A43

    Plasmid

    #132030

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A43 (SLC25A43 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pJUMP29-1A(lacZ)

    Plasmid

    #126978

    Purpose

    Level 1 vector. With lacZ as alternative cloning reporter. OriV 9 (pBBR322/ROP; medium copy number).

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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39. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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40. pCMV-PE7

    Plasmid

    #214812

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge