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pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3h

Plasmid

#208699

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3h

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33Y)-pcw107

Plasmid

#64615

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Mutation

S33Y

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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murine TCR OTI-2A.pMIG II

Plasmid

#52111

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

T2A (from Thosea asigna virus)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_D210N

Plasmid

#111904

Purpose

Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has Service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-NLS-6xHis

Plasmid

#62933

Purpose

Expression of Cas9-NLS-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9-NLS-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRiA4-VIR

Plasmid

#138193

Purpose

Virulence helper compatible with pCambia binary vectors.

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

vir gene cluster

Use

Vir gene expression

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pA-His6-Tn5

Plasmid

#171871

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Protein A and hyperactive Tn5 transposase

Tags

His-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IGF1R

Plasmid

#116752

Purpose

Lentiviral expression of IGF1R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IGF1R (IGF1R Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma2-GFP2

Plasmid

#166774

Purpose

Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma2-GFP2 (GNG2 Human, Synthetic)

Expression

Mammalian

Mutation

Contains an N-terminal GFP2 and GSAG linker

Promoter

CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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GPI-RpHLuorin2

Plasmid

#171721

Purpose

GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to GPI anchor

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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Cre-IRES-PuroR

Plasmid

#30205

Purpose

Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

Depositor

Darrell Kotton

Article

Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

Insert

Cre recombinase (cre Enterobacteria phage P1)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-FBL

Plasmid

#63672

Purpose

PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

EGFP-2A-PuroR

Use

CRISPR

Promoter

Promoterless

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgRNA

Plasmid

#124845

Purpose

Vector for FLP-dependent expression of SaCas9 with gRNA

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SauCas9 gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA4mt

Plasmid

#140463

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA4mt

Tags

Myc

Expression

Mammalian

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-PFKFB3

Plasmid

#23668

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKFB3 (PFKFB3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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mcherry-PH

Plasmid

#36075

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

PH domain of PLCdelta1 (PLCD1 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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