8,866 results

1. p21-Reporter

   Plasmid

   #52432

   Purpose

   DNA damage inducible Red Fluorescent Protein for mammalian expression

   Depositor

   Joel Voldman

   Article

   Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

   Insert

   p21-p53 Tandem Response Element Promoter
   

   Tags

   TurboRFP

   Expression

   Mammalian

   Promoter

   13X repeated p21-p53 response element

   Available Since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN11

   Plasmid

   #84455

   Purpose

   shuttle plasmid for sarA P1-mCherry expression

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   mCherry
   

   Use

   Shuttle vector e.coli-s.aureus, expression of sgf…

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. murine TCR OTI-2A.pMIG II

   Plasmid

   #52111

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   T2A (from Thosea asigna virus)

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. HP17_KO7

   Plasmid

   #120346

   Purpose

   Helper plasmid for production of phagemid ssDNA, based on M13KO7

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Coding part of the M13KO7 helper phage genome
   

   Expression

   Bacterial

   Promoter

   native phage promoters

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

   Plasmid

   #107942

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
   

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R, and N282A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAF0687 Pa01_attP-mCherry donor

   Plasmid

   #193466

   Purpose

   Promoterless mcherry donor for Pa01

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry- SEC61B

   Plasmid

   #121160

   Purpose

   Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B )
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. DLX2-hygro

   Plasmid

   #97330

   Purpose

   Generating lentiviruses to express Dlx2 and hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   DLX2 (Dlx2 Mouse)
   

   Use

   Lentiviral

   Promoter

   TetO

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Expression

   Bacterial

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

    Plasmid

    #60906

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSB819-PKR-hum

    Plasmid

    #20030

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

    Insert

    Protein kinase R (EIF2AK2 Human)
    

    Expression

    Yeast

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetO-Ascl1-puro

    Plasmid

    #97329

    Purpose

    Generating lentiviruses to express Ascl1 and puromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Promoter

    TET-ON

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT/Caggs-NRASV12

    Plasmid

    #20205

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    NRas (NRAS Human)
    

    Use

    Transposon

    Mutation

    G12V

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDESTmycYBX1

    Plasmid

    #19878

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Y box binding protein 1 (YBX1 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMIH-TOMM20-Halo

    Plasmid

    #111626

    Purpose

    Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    TOMM20 (Tomm20 Mouse)
    

    Use

    Retroviral

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Tags

    6xHis and V5

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SunTagng22aa

    Plasmid

    #106438

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_3xFlagEzh2 WT

    Plasmid

    #173717

    Purpose

    WT EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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