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20,135 results

Showing: 9631 - 9660 of 20135 results

  3. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid
    #140983
    Purpose
    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha11-RLuc8 (GNA11 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140983-plasmid-map-sequence-id-300767

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  4. pcDNA3-GFP-MT2A

    Plasmid
    #11613
    Depositor
    Steven Johnson
    Insert
    metallothionein 2A (MT2A Human)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    11613-plasmid-map-sequence-id-365342

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  12. pcDNA3-mNeptune2-N

    Plasmid
    #41645
    Purpose
    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    mNeptune2-Dronpa145N
    Expression
    Mammalian
    Mutation
    K145N on Dronpa (allows control of photochromic F…
    Promoter
    CMV
    Available Since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41645-plasmid-map-sequence-id-296256

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  18. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158762-plasmid-map-sequence-id-309495

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  20. pGEXTK-Pak1 70-117

    Plasmid
    #12217
    Depositor
    Jonathan Chernoff
    Insert
    Pak1 (PAK1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    PBD (p21-binding domain), aa 70-117
    Available Since
    Aug. 11, 2006
    Availability
    Academic Institutions and Nonprofits only
    12217-plasmid-map-sequence-id-166258

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  24. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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Showing: 9631 - 9660 of 20135 results