8,883 results
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p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Orai1 CFP
Plasmid#19757DepositorAvailable SinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependent Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-A2A-adenosine receptor
Plasmid#66995PurposeA2A-adenosine receptor tagged with HaloTag on amino terminusDepositorInsertHaloTag-A2A-adenosine receptor (ADORA2A Human)
TagsHuman Growth hormone Signal Sequence- HaloTagExpressionMammalianMutationThe signal sequence from human growth hormone was…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-gRNA-EF1a-EGFP-CAAX
Plasmid#167936PurposegRNA entry vectorDepositorInsertU6-gRNA backbone
UseLentiviralPromoterU6Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ190
Plasmid#162667PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSimple-miR302/367
Plasmid#98748PurposeEpisomal reprogrammingDepositorAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DeAct-SpvB
Plasmid#89446PurposeExpresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cellsDepositorInsertDeAct-SpvB (spvB Salmonella enterica)
TagsEGFPExpressionMammalianMutationTruncation spanning SpvB amino acids 375-591 (mon…PromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x2
Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Myosin-IIA-GFP
Plasmid#38297DepositorAvailable SinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXPR_011
Plasmid#59702PurposeThis lentiviral vector can be used to assay Cas9 activity.DepositorInsertEGFP sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK-GFP.WPRE
Plasmid#12252Purpose3rd generation lentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB
Plasmid#110820PurposeConventional dCas9 repressor-dCas9-KRABDepositorInsertdCas9-KRAB
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Nesprin3-C-18
Plasmid#54203PurposeLocalization: Nuclear Membrane , Excitation: 487, Emission: 509DepositorAvailable SinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSmartBAC-S
Plasmid#107268PurposeBroad host range bacterial artificial chromosome vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-K-GECO1
Plasmid#105865PurposeMammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1DepositorInsertK-GECO1
ExpressionMammalianAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plenti-ARE-HSVTK-luc
Plasmid#161789PurposeExpresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markersDepositorInsertsLuciferase-T2A-HSV-TK
mCherry P2A blasticidin
UseLentiviral and LuciferaseExpressionMammalianPromoterEF1a and Murine Gclc AREAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NES
Plasmid#168301PurposeChemogenetic fluorescent H2O2 reporter targeted to the cytoplasmDepositorInsertHyPer7.2DAAO-NES
ExpressionMammalianPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A Q67L
Plasmid#28049DepositorAvailable SinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxExpressionMammalianAvailable SinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKK-BiFC-Venus
Plasmid#105804PurposeConcomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.DepositorTypeEmpty backboneUseFlp-in competentTagsVN173 (I152L); VC155ExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-ER-5
Plasmid#55294PurposeLocalization: Endoplasmic Reticulum, Excitation: 399, Emission: 456DepositorAvailable SinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV-mCherry-(F30-2xPepper)10
Plasmid#129401PurposemCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertmCherry-(F30-2xPepper)10
ExpressionMammalianPromoterCMVAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR9-YFP
Plasmid#13642DepositorAvailable SinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MERTK
Plasmid#23900DepositorInsertMERTK (MERTK Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-delta30-TFEB
Plasmid#44445DepositorAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
ExpressionBacterialMutationA309GAvailable SinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2Cre
Plasmid#82415PurposeLentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning siteDepositorInsertCre Recombinase
UseCRISPR, Cre/Lox, and LentiviralMutationMutated BsmBI cut sitePromoterEFS (P2A)Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24b-APEX2
Plasmid#111702PurposeFor IPTG-inducible expression of APEX2 in E. coliDepositorInsertAPEX2
Tags6xHis and V5ExpressionBacterialPromoterT7 promoterAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.MaCPNS2
Plasmid#185137Purposenon-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS2 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
hIRE1a wt
Plasmid#20744DepositorAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH1
Plasmid#108699PurposeFor expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cellsDepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-c-Met
Plasmid#31784DepositorAvailable SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB-wt
Plasmid#58905Purposemammalian expression of wild type human RbDepositorAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycYBX1
Plasmid#19878DepositorAvailable SinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-Halotag-FRB-EX-nes_pLX304
Plasmid#120913PurposeLentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosolDepositorInsertHA-Halotag-FRB-EX-NES
UseLentiviralExpressionMammalianAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-GFP
Plasmid#86145PurposeLentiviral vector expressing Cas9-GFPDepositorInsertCas9-GFP
UseCRISPR and LentiviralTagsFLAG and GFPExpressionMammalianPromoterEFSAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
VCP(wt)-EGFP
Plasmid#23971DepositorInsertValosin containing protein (VCP Human)
Tags6xHis, EGFP, and HAExpressionMammalianMutationnoneAvailable SinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET_mNG2(1-10)_32aalinker_mNG2(11)
Plasmid#82611PurposeBacterial expression vector for split mNeonGreen2 screeningDepositorInsertmNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
ExpressionBacterialMutationsplit before mutationsPromoterT7Available SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Klotho (secreted)
Plasmid#17713DepositorAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIP
Plasmid#50015PurposeMammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorAvailable SinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPSU1
Plasmid#89439PurposeHigh copy number plasmid 1 to prepare Penn State DNA molecular weight laddersDepositorInsertsExpressionBacterialAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-V5-TurboID
Plasmid#166971PurposeExpresses ER targeted TurboID for secretome biotinylationDepositorInsertSec61b-V5-TurboID
TagsV5ExpressionMammalianPromoterCMV promoterAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only
