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165,221 results

Showing: 1951 - 2000 of 165221 results

  1. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available Since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62723-plasmid-map-sequence-id-166279

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  3. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212962
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_non-targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212962-plasmid-map-sequence-id-422485

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  9. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

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  12. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

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  20. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  21. pCW57.1_blasti

    Plasmid
    #194067
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194067-plasmid-map-sequence-id-380706

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  29. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210704-plasmid-map-sequence-id-416247

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  30. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  32. M13cp-dg3 hp

    Plasmid
    #218095
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218095-plasmid-map-sequence-id-432463

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  34. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50590-plasmid-map-sequence-id-210724

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  41. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    P45
    Use
    Synthetic Biology
    Available Since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  43. pLX302

    Plasmid
    #25896
    Purpose
    3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.
    Depositor
    David Root
    Article
    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 30, 2010
    Availability
    Academic Institutions and Nonprofits only
    25896-plasmid-map-sequence-id-364646

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  44. sUPRa

    Plasmid
    #223242
    Purpose
    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels
    Depositor
    Colin Akerman
    Article
    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10
    Insert
    sUPRa
    Use
    AAV
    Promoter
    Custom
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    223242-plasmid-map-sequence-id-450772

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  48. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

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  50. MCP-NMS

    Plasmid
    #210705
    Purpose
    This plasmid express EF1a promoter driven MCP-NMS transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210705-plasmid-map-sequence-id-416249

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Showing: 1951 - 2000 of 165221 results