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167,150 results

Showing: 1951 - 2000 of 167150 results

  8. GPR50-Tango

    Plasmid
    #66361
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR50 (GPR50 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. LentiV_Neo_SIK3_CR_T221E

    Plasmid
    #108108
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108108-plasmid-map-sequence-id-207841

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  14. pFH103

    Plasmid
    #128208
    Purpose
    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128208-plasmid-map-sequence-id-252907

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  15. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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  23. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4

    Plasmid
    #193345
    Purpose
    tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Sox2-17-t2a-Klf4 (Klf4, Pou5f1, Sox2 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    TetO mini CMV
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193345-plasmid-map-sequence-id-410597

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  30. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  34. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

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  35. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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  40. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid
    #141237
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    GCaMP6f
    Use
    AAV and Cre/Lox
    Tags
    6XHIS and Xpress
    Promoter
    Human elongation factor-1 alpha (EF-1 alpha)
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    141237-plasmid-map-sequence-id-431695

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Showing: 1951 - 2000 of 167150 results