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Addgene

We narrowed to 3,985 results for: LYC

Showing: 2751 - 2800 of 3985 results
  1. GB4540

    Plasmid
    #215259
    Purpose
    Module for the expression of the N. nambi HISPS gene under the regulation of Solanum lycpersicum DFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19
    Depositor
    Insert
    PDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB

    Plasmid
    #225488
    Purpose
    Tet-on lentiviral vector for controlled expression of GDNF
    Depositor
    Insert
    Tetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse phosphoglycerate kinase 1
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pET28Man

    Plasmid
    #203399
    Purpose
    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes
    Depositor
    Insert
    man2A
    Use
    Tags
    H6
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. E1_pENTR_L1-L4_NI_G1333-DddtoxA-C

    Plasmid
    #171724
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. E1_pENTR_L1-L4_NN_G1333-DddtoxA-C

    Plasmid
    #171728
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. E3_pENTR_L3-L2_NG_G1333-DddtoxA-C

    Plasmid
    #171732
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. E1_pENTR_L1-L4_NI_G1333-DddtoxA-N

    Plasmid
    #171725
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. E1_pENTR_L1-L4_NN_G1333-DddtoxA-N

    Plasmid
    #171729
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. E3_pENTR_L3-L2_NG_G1333-DddtoxA-N

    Plasmid
    #171733
    Purpose
    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. C513-E06: attB5r-pA-polyhedrinp>-attB1r

    Plasmid
    #162948
    Purpose
    Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Insert
    AcMNPV polyhedrin promoter with upstream polyA sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pDGE473

    Plasmid
    #153264
    Purpose
    M1E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pDGE500

    Plasmid
    #153273
    Purpose
    M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pDGE502

    Plasmid
    #153275
    Purpose
    M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. ORF18(L151A_G152A)-3xFLAG pCDNA4

    Plasmid
    #129813
    Purpose
    Expresses KSHV ORF18 (L151A_G152A) with Cterm 3xFLAG tag in mammalian cells
    Depositor
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Leucine 151 to Alanine and Glycine 152 to…
    Promoter
    CMV
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. ORF18(G145A_N146A)-3xFLAG pCDNA4

    Plasmid
    #129812
    Purpose
    Expresses KSHV ORF18 (G145A_N146A) with Cterm 3xFLAG tag in mammalian cells
    Depositor
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Glycine 145 to Alanine and Asparagine 146…
    Promoter
    CMV
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. ORF18(W170A_G171A)-3xFLAG pCDNA4

    Plasmid
    #129815
    Purpose
    Expresses KSHV ORF18 (W170A_G171A) with Cterm 3xFLAG tag in mammalian cells
    Depositor
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Tryptophan 170 to Alanine and Glycine 171…
    Promoter
    CMV
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. pAL800

    Plasmid
    #119306
    Purpose
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Depositor
    Insert
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    amino acids 1 through 171 deleted, and mutatioms …
    Promoter
    lac
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  19. pJK381

    Plasmid
    #71573
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)
    Depositor
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to glycine
    Promoter
    recA
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
  20. miR-451 Trap

    Plasmid
    #61487
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Insert
    miR-451 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2751 - 2800 of 3985 results