4,114 results

1. hSERT pcDNA3

   Plasmid

   #15483

   Depositor

   Randy Blakely

   Article

   Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

   Insert

   hSERT (SLC6A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE PGK-GFP-IRES-LUC-W

   Plasmid

   #46793

   Purpose

   2nd generation lentiviral vector

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Inserts

   PGK
   eGFP (eGFP )
   IRES
   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMVHA EED wt

   Plasmid

   #24231

   Purpose

   Mammalian expression of human EED with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EED (EED Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Flag-HA-OTUD5

   Plasmid

   #22610

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   OTUD5 (OTUD5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-PGC1

   Plasmid

   #4

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MLM3613

   Plasmid

   #42251

   Purpose

   Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-Flpe:GFP

   Plasmid

   #13788

   Purpose

   Mammalian expression of enhanced form of Flp recombinase fused to GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Flpe-GFP fusion protein
   

   Use

   Flp/frt

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABE YAP1

   Plasmid

   #15682

   Depositor

   Joan Brugge

   Article

   Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   YAP (YAP1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-IP GFP-WIPI-1

    Plasmid

    #38272

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid

    #48141

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. FLHKI-pGFPN3

    Plasmid

    #21917

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKI in pGFP-N3 (HK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is full length human HKI in pGFP-N3

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid

    #46912

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-VP64-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    3xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

    Plasmid

    #31484

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    EF1s HA NLS Sce(opt) T2A IFP1.4
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    "Opt" indicates the I-SceI coding seque…

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. human STIM1 YFP

    Plasmid

    #19754

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYIC

    Plasmid

    #18673

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Type

    Empty backbone

    Use

    Tags

    3Myc-EYFP-HA-His6 and ECFP-HA-His6

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY004 (pcDNA3.1-hFnCpf1)

    Plasmid

    #69976

    Purpose

    Expresses humanized FnCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hFnCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEos3.2-N1

    Plasmid

    #54525

    Purpose

    Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson, Tao Xu

    Article

    Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

    Type

    Empty backbone

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pLenti CMV/TO Neo empty (w215-1)

    Plasmid

    #17485

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cav1-GFP

    Plasmid

    #14433

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Caveolin-1 (CAV1 Canis familalis)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pH2B_mCherry_IRES_puro2

    Plasmid

    #21045

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B (H2BC16P Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBA439

    Plasmid

    #85967

    Purpose

    Perturb-seq vector backbone

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLL7.0: Venus-iLID-Mito (From ActA)

    Plasmid

    #60413

    Purpose

    Mammalian Expression of Venus-iLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-Peredox-mCherry-NLS

    Plasmid

    #32384

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Use

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEP4 E02S EM2K

    Plasmid

    #20923

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MSCV Myc T58A puro

    Plasmid

    #18773

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    myc T58A (MYC Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-DIO EYFP

    Plasmid

    #27056

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 mCherry LIC cloning vector (6B)

    Plasmid

    #30125

    Purpose

    Empty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HDAC8 Flag

    Plasmid

    #13825

    Depositor

    Eric Verdin

    Article

    Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

    Insert

    HDAC8 (HDAC8 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. xCas9 3.7

    Plasmid

    #108379

    Purpose

    Mammalian expression vector for xCas9 3.7

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG HRP-TM

    Plasmid

    #44441

    Depositor

    Alice Ting

    Article

    Rhee et al Science. 2013 Jan 31.

    Insert

    Horseradish peroxidase fused to the transmembrane domain of PDGFR
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-EF1a-mCherry-IRES-Flpo

    Plasmid

    #55634

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV1

    Inserts

    mCherry
    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1 puro Nodamura B2

    Plasmid

    #17228

    Depositor

    Christopher Sullivan

    Article

    Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

    Insert

    Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2 silent point mutations

    Promoter

    Available since

    June 22, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pLenti SpBsmBI sgRNA Hygro

    Plasmid

    #62205

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBABEpuro-gateway

    Plasmid

    #51070

    Purpose

    gateway adapted mammalian expression vector

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. YAP1-V5 in pLX304

    Plasmid

    #42555

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti PGK Blast V5-LUC (w528-1)

    Plasmid

    #19166

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-PTEN

    Plasmid

    #28298

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLentiCas9-T2A-BFP

    Plasmid

    #78547

    Purpose

    Cas9 protein with BFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3-EGFP-Cdc42(T17N)

    Plasmid

    #12601

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (T17N) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. FR_HNF1A

    Plasmid

    #31104

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. TUBB5-Halo

    Plasmid

    #64691

    Purpose

    Expresses beta-tubulin labeled with Halo-tag

    Depositor

    Yasushi Okada

    Article

    Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

    Insert

    TUBB
    

    Use

    Tags

    Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEGFP-C1-FLAG

    Plasmid

    #46956

    Purpose

    Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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