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MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Article

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMD2-VSVGmut

Plasmid

#182229

Purpose

Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

VSVGmut

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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v5 ABE-eVLP

Plasmid

#228467

Purpose

Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–ABE8e

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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[#GS24 -LV] Syn1-GFP-OMP25

Plasmid

#233566

Purpose

Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

eGFP (eGFP Synthetic, Aequorea victoria)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pPGK-SB13

Plasmid

#236078

Purpose

SB13 transposase, for use with Sleeping Beauty transponson plasmids

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Insert

SB13 transposase

Use

Sleeping beauty (sb) transposase plasmid

Promoter

PGK

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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Tcf/Lef:H2B-GFP

Plasmid

#32610

Depositor

Anna-Katerina Hadjantonakis

Article

Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

Insert

Tcf/Lef:H2B-GFP

Promoter

hsp68

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225242

Purpose

Mammalian expression of iPASnFR-L in the cytosol

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

Plasmid

#225241

Purpose

Mammalian expression of iPASnFR-H in the cytosol

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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MS03

Plasmid

#164526

Purpose

Expresses Sonic Hedgehog

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Sonic Hedgehog (SHH Human)

Use

Lentiviral

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209723

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE NFkB-TA-LUC-UBC-GFP-W

Plasmid

#49343

Purpose

pHAGE NFkB-TA-LUC-UBC-GFP-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

eGFP (eGFP )
Luciferase
NFkB consensus binding sequence
TA
UBC

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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LSL-Cas9-Rosa26TV

Plasmid

#61408

Purpose

Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

Cas9
EGFP

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

3xFLAG and P2A

Expression

Mammalian

Mutation

human codon optimized

Promoter

CAG

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ATR Kinase dead

Plasmid

#31612

Depositor

Aziz Sancar

Article

Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Mutation

K2327R (kinase dead)

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only

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rAAV2/MG1.2

Plasmid

#184541

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.2 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, Q588T; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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p2514-pHAGE2-EF1aL-tdTomato-W

Plasmid

#248971

Purpose

Lentiviral expression of tdTomato

Depositor

Darrell Kotton

Insert

tdTomato

Use

Lentiviral

Promoter

EF1aL

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-EGFP

Plasmid

#174186

Purpose

Express EGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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