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Showing: 101 - 120 of 158569 results

  3. pLEX_307

    Plasmid
    #41392
    Purpose
    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41392-plasmid-map-sequence-id-331248

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  5. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

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  10. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

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  11. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  15. pMSP1D1

    Plasmid
    #20061
    Purpose
    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477
    Insert
    MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    deletion mutant (1-11) of MSP1; His-tag on N-term…
    Promoter
    Available since
    March 16, 2009
    Availability
    Academic Institutions and Nonprofits only
    20061-plasmid-map-sequence-id-354445

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  16. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  18. pAAV2/rh10

    Plasmid
    #112866
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap-rh10
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Truncated P5
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112866-plasmid-map-sequence-id-339914

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Showing: 101 - 120 of 158569 results