166,224 results

1. pCAG-VSVG

   Plasmid

   #35616

   Depositor

   Arthur Nienhuis, Patrick Salmon

   Insert

   VSV G protein
   

   Expression

   Mammalian

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV.CBA.eGFP.2A.P301L-Tau

   Plasmid

   #140425

   Purpose

   AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

   Depositor

   Bradley Hyman

   Article

   Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

   Insert

   eGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
   

   Use

   AAV

   Tags

   eGFP

   Mutation

   changed Proline 301 to Leucine

   Promoter

   CBA

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-p53

   Plasmid

   #12091

   Depositor

   Tyler Jacks

   Article

   Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

   Insert

   p53 (TP53 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   72R variant of p53

   Available Since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

   Plasmid

   #108542

   Purpose

   Lentivirus vector expressing Luciferase with blasticidin resistance

   Depositor

   Javier Alcudia

   Inserts

   Luciferase
   Neomycin
   

   Use

   Lentiviral

   Promoter

   EF1apha and PGK

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

   Plasmid

   #71396

   Purpose

   2Gi2R assay

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Inserts

   2xGFP:NES
   2xRFP:NLS
   

   Use

   Lentiviral

   Tags

   IRES

   Expression

   Mammalian

   Promoter

   EF1alpha and IRES

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. XLone-GFP

   Plasmid

   #96930

   Purpose

   Tunable and temporal expression control of GFP

   Depositor

   Xiaojun Lian

   Article

   Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

   Insert

   Green Fluorescent Protein
   

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-PGK-Hygro-PIP-FUCCI

   Plasmid

   #138714

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-CMV-jGCaMP8m

    Plasmid

    #162372

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-S33Y Beta-catenin

    Plasmid

    #19286

    Purpose

    Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S33Y (presumptive GSK3β phosphorylation site)

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTRE-BI-osTIR1

    Plasmid

    #207840

    Purpose

    Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    osTIR
    mCherry
    mAID_-VHH-GFP4
    

    Tags

    3X HA-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFX-Tom20-EGFP

    Plasmid

    #174186

    Purpose

    Express EGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of TOM20

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. [#GS7-LV] DsRed GFP TEVp Zeo Switch - Recipient

    Plasmid

    #233507

    Purpose

    Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    DsRed loxP eGFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK-flag-GFP11

    Plasmid

    #78590

    Purpose

    Expression flag-GFP 11 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-GFP 11
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

18. ARTi

    Plasmid

    #214064

    Purpose

    Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF1-Pls3-sfGFP

    Plasmid

    #205750

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Expression

    Bacterial

    Promoter

    Pls3 (Phyperspank mutant, IPTG inducible)

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid

    #105648

    Purpose

    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

    Depositor

    Sandra Rieger

    Insert

    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge