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177,146 results

Showing: 3101 - 3150 of 177146 results

  1. mEmerald-Sec61b-C1

    Plasmid
    #90992
    Purpose
    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells
    Depositor
    Jennifer Lippincott-Schwartz
    Publication
    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mEmerald-Sec61b (SEC61B Human, Synthetic)
    Tags
    mEmerald (green fluorescence)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    90992-plasmid-map-sequence-id-179977

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  3. pAnc80L65

    Plasmid
    #68837
    Purpose
    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
    Depositor
    Luk Vandenberghe
    Publication
    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1
    Insert
    Ancestral AAV Capsid Anc80L65
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    Rep2
    Available since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    68837-plasmid-map-sequence-id-121219

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  4. pSBbi-Bla

    Plasmid
    #60526
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    60526-plasmid-map-sequence-id-97520

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  5. mCherry-ZO1-C-14

    Plasmid
    #55165
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZO1 (TJP1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    55165-plasmid-map-sequence-id-194785

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  19. pGL3 Basic Vector

    Plasmid
    #212936
    Purpose
    Firefly luciferase vector for investigating regions controlling transcription
    Depositor
    Debrya Groskreutz
    Type
    Empty backbone
    Use
    Luciferase
    Mutation
    WT
    Available since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    32 Articles
    212936-plasmid-map-sequence-id-419196

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  20. pCMV-GenEPi

    Plasmid
    #140236
    Purpose
    Mammalian expression of Piezo1-based fluorescent force sensor GenEPi
    Depositor
    Periklis Pantazis
    Publication
    Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
    Tags
    GCaMP 6s RS1 EF4
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    140236-plasmid-map-sequence-id-308737

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  22. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

    Plasmid
    #141379
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    13 Articles
    141379-plasmid-map-sequence-id-288091

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  24. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    122650-plasmid-map-sequence-id-437776

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  32. pICE-HA-MRE11-WT

    Plasmid
    #82033
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    82033-plasmid-map-sequence-id-490398

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  36. APEX2 - Actin in pEGFP

    Plasmid
    #66172
    Purpose
    APEX2 fused to the N-terminus of human beta actin
    Depositor
    Alice Ting
    Publication
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    APEX2 - human beta Actin (ACTB Human, Synthetic)
    Tags
    Flag epitope tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    APEX2 contains 4 mutations relative to wt soybean…
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    66172-plasmid-map-sequence-id-230313

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Showing: 3101 - 3150 of 177146 results