171,027 results
-
Plasmid#221005PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.DepositorInsertFluc-SMN2E7-STOP-Nluc
ExpressionMammalianPromoterCMVAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneExpressionBacterialPromoterhyper-pSpankAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p300 core-dCas9-CBP core
Plasmid#179559Purposeencodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertUseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvLEAM
Plasmid#90033PurposeTo express His-tagged RvLEAM in bacteriaDepositorInsertRvLEAM
TagsHisExpressionBacterialPromoterT7Available SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-synORP1
Plasmid#191701PurposeTi plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.DepositorInsertchl-roGFP2-synORP1
UseBinary agrobacterium ti plasmidExpressionPlantPromoterCaMV 35S promoterAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRB_p2_TCRA
Plasmid#131034PurposeExpresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptideDepositorArticleAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2154
Plasmid#141947PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Foxo1
Plasmid#17551DepositorAvailable SinceOct. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-EGFP
Plasmid#84041Purpose3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseLentiviralExpressionMammalianPromoterTRE-CMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK1254
Plasmid#133057PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmRuby2ExpressionMammalianPromoterEF1AAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_neomycin
Plasmid#65312PurposeCytoplasmic ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
ExpressionMammalianMutationStreptavidin in its cytoplasmid partPromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mouse E-cadherin GFP (423)
Plasmid#67937PurposeMammalian expression of E-cadherin-GFPDepositorAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-DIO-hM4Di-mCherry
Plasmid#81008PurposeCre-dependent expression of hM4Di-mCherry under CBA promoterDepositorInserthM4D(Gi)-mCherry
UseAAVTagsmCherryPromoterCBAAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 Full Length-6MT
Plasmid#41728DepositorInsertNotch-1 Full Length (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationL1997V; Contains murine Notch-1 protein up to ami…Available SinceDec. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMK347 (BSD-P2A-mAID)
Plasmid#121181PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK286 (mAID-NeoR)
Plasmid#72824PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Neo
UseCRISPRTagsmAIDExpressionMammalianAvailable SinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_post
Plasmid#119736PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA
UseAAVTagsFLAGExpressionMammalianPromoterhSyn-1Available SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
m168
Plasmid#34886DepositorInsertSindbis virus envelope protein
ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET iGluSnFR3 v82
Plasmid#175187PurposeFluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.DepositorInsertiGluSnFR3 v82
UseSynthetic BiologyTagsHis tag, T7 leader, Xpress epitopeExpressionBacterialPromoterT7Available SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d
Plasmid#108305PurposeExpresses E. coli codon optimized RspCas13d in the pET28a backbone.DepositorInsertRspCas13d
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-p300_Blast
Plasmid#192653Purpose3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance markerDepositorInsertdCas9-p300
UseCRISPR and LentiviralExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGuide
Plasmid#64711PurposeCloning and expression of Streptococcus pyogenes guide RNADepositorInsertStreptococcus pyogenes guide RNA
UseCRISPRExpressionMammalianPromoterhU6Available SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1-F-tractin-mCherry
Plasmid#155218PurposeMonitor global F-actin distributionDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
TagsmCherryExpressionMammalianAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP
Plasmid#20672PurposeDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES EGFPExpressionMammalianAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
ExpressionMammalianAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai2
Plasmid#196049PurposeEncodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-DddA11_NT
Plasmid#232441PurposeAnti-rabbit IgG Fc specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)DepositorInsertnbOcIgG-DddA11_N
Available SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRTH
Plasmid#136919PurposeExpresses BE4max-NRTH in mammalian cellsDepositorInsertBE4max-NRTH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-EGFP-WPRE-pA
Plasmid#37091PurposeExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertd1eGFP
UseAAV and Cre/Lox; Cre-offPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-InC
Plasmid#83959PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.DepositorInsertInternal Control sequence
ExpressionBacterialAvailable SinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyExpressionYeastPromoterPGK1, TDH3, TIP1, and ZEO1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mouse IL-6 (1240)
Plasmid#66908PurposeMammalian expression of IL-6DepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-m
Plasmid#136294Purposeexpresses ABE8.8-m in mammalian cellsDepositorInsertABE8.8-m
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
RV-Syn-Dio-yfp_2a_elfn1
Plasmid#98177PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-elfn1DepositorAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LeGO-G2
Plasmid#25917DepositorInsertSFFV promoter, eGFP
UseLentiviralExpressionMammalianAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt1
Plasmid#66830PurposeMammalian Expression, Bacterial ExpressionDepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/11
Plasmid#240486PurposeAAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid proteinDepositorInsertAAV2 Rep and AAV11 Cap
UseAAVExpressionMammalianAvailable SinceJuly 25, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-myrSNAP
Plasmid#60098PurposeExpresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditionalDepositorInsertsSNAP-tag
histone-GFP
UseAAV and Mouse TargetingExpressionMammalianAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.RMacL3-01.EGFP.BCA5.WPRE
Plasmid#225685PurposeL3 pyramidal neuron Enhancer to drive transgene expressionDepositorInsertRMacL3-01.EGFP.BCA5
ExpressionMammalianAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-N
Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1
Plasmid#233078PurposeExpression vector for sgRNA-S1b and eCas12f1DepositorInsertU6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianPromoterhU6, CBhAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_pre
Plasmid#119738PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA_pre
UseAAVExpressionMammalianPromoterhsyn1Available SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDest17_MAX
Plasmid#234044PurposeBacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage siteDepositorAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman 7SKAvailable SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.GFP
Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGPromoterEFS and hU6Available SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-roGFP
Plasmid#202464PurposeExpresses redox sensitive GFP (roGFP) with T7 inductionDepositorInsertreduction-oxidation sensitive green fluorescent protein
Tags6X-HisExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBE45
Plasmid#174655PurposeCAPTURE method receiver plasmid for Streptomyces heterologous expressionDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
[#GS5-RV] MitoTRACER-Donor
Plasmid#233505PurposeRetroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cellsDepositorInsertMitoTRACER-Donor
UseRetroviral and Synthetic BiologyTagsGFP11 and HA TagExpressionMammalianAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only