2,202 results
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Plasmid#117779PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Cavin4b-TurboID
Plasmid#163851PurposeIn vitro transcription of Cavin4b-TurboID. Parton lab clone JTADepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-CD44b-TurboID
Plasmid#163850PurposeIn vitro transcription of CD44b-TurboID. Parton lab clone JTJDepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-polg guide
Plasmid#107598PurposeAn entry vector with U6a promoter driving polg guide RNA expressionDepositorInsertpolg guide RNA sequence
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9
Plasmid#118842PurposeTargets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertcdkn2a gRNA (LOC100329528 Zebrafish)
UseCRISPR; Tol2Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mpeg:lamp2-mCherry-stop; cmlc2:GFP
Plasmid#213740PurposeExpresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis markerDepositorInsertlamp2-mCherry
UseZebrafishPromotermpeg1.1Available SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZE31 Prickle-1-pCS2P+
Plasmid#17072DepositorInsertPrickle-1 (prickle1a Zebrafish)
UseZebrafish expressionAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnk18
Plasmid#209000PurposeRiboprobe generationDepositorAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-CAAX-SV40
Plasmid#117815PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsCAAXAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E Lck
Plasmid#135201PurposeT-cell markerDepositorInsertLck promoter
UseSynthetic BiologyAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-B-actin
Plasmid#117814PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish aBb crystallin 2kb promoter/AcGFP
Plasmid#98099PurposeZebrafish aBb crystallin 2kb promoter segment driving GFP expressionDepositorInsertAlpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPPromoterDanio alpha Bb-crystallin 2 kb fragment (-2092/-1)Available SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-mRFP
Plasmid#117772PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -1
Plasmid#180011PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H
Plasmid#187186PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-B-actin
Plasmid#117808PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab13 in pCSDEST2
Plasmid#140879PurposeExpression of zebrafish GFP-tagged Rab13 in mammalian cells.DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato
Plasmid#172535PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistanceDepositorInsert-3.5ubb:plin2-tdtomato
MutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-CAAX-B-actin
Plasmid#117810PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsCAAXAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DestFLAGHA_RNAseH1
Plasmid#65783Purposeconstitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cellsDepositorAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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ZP46 Prickle-2
Plasmid#16896DepositorInsertPrickle-2 (prickle2b Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pTone-gata2aECE-Kaede
Plasmid#132976Purposegata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backboneDepositorInsertsKaede
gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pT2-cryR;cldn5bP1Egfp
Plasmid#90158Purposecontains cldn5b specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCS2+-Pard3-EGFP-PIF6
Plasmid#154914PurposeFull length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).DepositorInsertpar-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
UseXenopus/avian/zebrafishTags1-100 amino acids of Arabidopsis PIF6 and EGFPExpressionMammalianPromoterCMVAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfEpoa
Plasmid#65611PurposeExpress soluble His-tagged zebrafish erythropoietin in E. coliDepositorAvailable SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
hsp70: mKate2-DN Kif5a
Plasmid#105972Purposeheat shock inducible transgenesisDepositorAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zAsCpf1
Plasmid#107185Purposeplasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)DepositorInsertAsCpf1
UseCRISPRTagsNLS-3xHAAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5E_(-3kb)nrg1 type II
Plasmid#199393PurposepDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequenceDepositorInsert(-3kb)nrg1 type II
UseGateway CloningAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1- Ncad-GFP
Plasmid#35676DepositorAvailable SinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZE26 Stbm-N-6myc-pCS2+
Plasmid#17068DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZE45 Dvl-2 pCS2P+
Plasmid#17086DepositorInsertDvl-2 (dvl2 Zebrafish)
UseZebrafish expressionAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorAvailable SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-ndufs2 guide
Plasmid#107601PurposeAn entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expressionDepositorInsertndufs2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
41 pBS119 d10L
Plasmid#13065DepositorInsertfli genomic (fli1 Zebrafish)
Available SinceJune 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-gal4-VP16-cmlc2-mRFP
Plasmid#117781PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKSII/INSeGFP
Plasmid#53787Purpose1 kb zebrafish insulin promoter driving eGFPDepositorInsertINS promoter/eGFP/pA
UseZebrafishTagseGFPExpressionBacterialPromoterzebrafish insulin/ZIPAvailable SinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
shha-pCRII
Plasmid#68962Purposefor antisense probe, NotI w/ Sp6DepositorInsertshha (shha Zebrafish)
Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-gcry-eGFP
Plasmid#117767PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-eGFP
Plasmid#117794PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-mcherry-2a-CDK2
Plasmid#121131Purposeneutrophil specific expressionDepositorInsertdre-WT CDK2 (cdk2 Zebrafish)
Tagsmcherry-2aExpressionBacterialPromoterneutrophil specific lyzCAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha A-crystallin 1kb promoter/AcGFP
Plasmid#98096PurposeaA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
UseGfp expressingTagsGFPPromoterDanio alpha A-crystallin 1 kb fragment (-1028/-1)Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISceI Dusp6:memGFP
Plasmid#32668PurposeExpresses membrane localized EGFP under the Dusp6 promoterDepositorInsertDusp6 promoter (dusp6 Zebrafish)
UseZebrafish transgenesisAvailable SinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKE13-ISceI-fabp10a:CreERT2
Plasmid#198242PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinaseDepositorInsertsUseZebrafish transgenesisAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only