177,146 results

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TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Publication

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Publication

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pJL-TRBO

Plasmid

#80082

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

Depositor

John Lindbo

Publication

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMAL-c2X

Plasmid

#75286

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Publication

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

32 Articles

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pCRISPRyl

Plasmid

#70007

Purpose

CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2015 Dec 29.

Inserts

Codon optimized Cas9 from S. pyogenes
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAAVS1-TLR targeting vector

Plasmid

#64215

Purpose

Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

traffic light reporter (TLR) (AAVS1 Human)

Use

Human targeting

Mutation

see publication for details

Promoter

CAG

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Publication

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-TOMM20-N-10

Plasmid

#55328

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TOMM20 (TOMM20 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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MAVS-WT

Plasmid

#52135

Purpose

Retroviral vector for expression of human MAVS in mammalian cells

Depositor

Jonathan Kagan

Publication

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS (MAVS Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-Rab35 WT

Plasmid

#47424

Depositor

Peter McPherson

Publication

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab35 (RAB35 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Publication

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMP31-1

Plasmid

#45905

Depositor

Harmit Malik

Publication

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mitochondrial antiviral signaling protein (MAVS Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCM157

Plasmid

#45863

Depositor

Mary Lidstrom

Publication

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Insert

cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

lac

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-HDAC4

Plasmid

#45636

Depositor

Tso-Pang Yao

Publication

Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HDAC4 (HDAC4 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Active Wnt3A-V5

Plasmid

#43810

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WNT3A (WNT3A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Ubc.Luc.IRES.Puro

Plasmid

#33307

Depositor

Linzhao Cheng

Publication

Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

Ubc

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBMPC3

Plasmid

#31367

Depositor

Aziz Sancar

Publication

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bmal1 (Bmal1 Mouse)

Tags

His

Expression

Mammalian

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV-Tag4A-MITF-M (wt)

Plasmid

#31151

Depositor

Yardena Samuels

Publication

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

WT

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Cofilin-pmCherryC1

Plasmid

#27687

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cofilin (Cfl1 Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LV-YFP

Plasmid

#26000

Depositor

Elaine Fuchs

Publication

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-YFP (H2BC21 A. victoria, Human)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-FRB

Plasmid

#25919

Depositor

Klaus Hahn

Publication

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FRB (MTOR Human)

Expression

Mammalian

Available since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
pCSDest

Plasmid

#22423

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

Enlarge
Flag-HA-COPS5

Plasmid

#22541

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

COPS5 (COPS5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pBiFC-CrN173

Plasmid

#22016

Depositor

Chang-Deng Hu

Publication

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CrN173

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE puro PPAR gamma 2

Plasmid

#8859

Depositor

Bruce Spiegelman

Publication

Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

Insert

PPAR gamma (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

Enlarge
pGBKCg

Plasmid

#20162

Depositor

Peter Uetz

Publication

Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

Type

Empty backbone

Tags

Gal4 DBD

Expression

Yeast

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pCMV-Cyclin D1

Plasmid

#19927

Depositor

Yue Xiong

Publication

Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cyclin D1 (CCND1 Human)

Expression

Mammalian

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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FU-tet-o-hSox2

Plasmid

#19779

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Sox2 (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
shp63alpha pLKO.1 puro

Plasmid

#19120

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p63 (TP63 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pAG426GPD-ccdB

Plasmid

#14156

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
pDW1775

Plasmid

#13456

Purpose

Plant ZFN expression vector.

Depositor

Daniel Voytas

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

AcV5

Expression

Plant

Available since

Dec. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pcDNA3-Casp3 C163A-myc

Plasmid

#11814

Depositor

Guy Salvesen

Publication

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

Myc

Expression

Mammalian

Mutation

C163A

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
896 pcDNA3 T7 Akt1

Plasmid

#9003

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

Enlarge
1319 pcDNA3 flag FKHRL1 AAA

Plasmid

#10709

Depositor

William Sellers

Publication

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKHRL1 AAA (FOXO3 Human)

Tags

flag

Expression

Mammalian

Mutation

AAA mutant (see map) - T32A, S253A, S315A

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
p1324 HPV-16 E7

Plasmid

#8643

Depositor

Peter Howley

Publication

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HPV-16 E7 (E7 Human papillomavirus)

Expression

Mammalian

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCAG-G-Flamp1

Plasmid

#188567

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Publication

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G-Flamp1

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Publication

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

Plasmid

#141367

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

Enlarge
pSin Helper

Plasmid

#127694

Purpose

Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Publication

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Glycoproteins

Use

Synthetic Biology

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

Enlarge
VPS13C^mClover3

Plasmid

#118760

Purpose

VPS13C internally tagged with mClover3 at residue 1914.

Depositor

Pietro De Camilli

Publication

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13C (VPS13C Human)

Tags

mClover3 inserted at position aa 1914

Expression

Mammalian

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pKM461

Plasmid

#108320

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor
SacRB

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Publication

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Expression

Bacterial

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pLentiPGK Blast DEST ERKKTRmRuby2

Plasmid

#90231

Purpose

Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Publication

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pC0056 - LwCas13a-msfGFP-NES

Plasmid

#105815

Purpose

Expresses active LwaCas13a-NES for mammalian RNA targeting

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NES and msfGFP-NES-3xHA

Expression

Mammalian

Promoter

EF-1a

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge

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