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169,365 results

Showing: 3501 - 3550 of 169365 results

  6. HA-CHMP4B

    Plasmid
    #139013
    Purpose
    Expresses HA-tagged human CHMP4B in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human CHMP4B
    Tags
    HA
    Expression
    Mammalian
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139013-plasmid-map-sequence-id-274403

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  8. pCW57-MCS1-2A-MCS2

    Plasmid
    #71782
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71782-plasmid-map-sequence-id-222919

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  11. pJR100

    Plasmid
    #187240
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available Since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    187240-plasmid-map-sequence-id-374768

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  16. pOpen-T5gene122

    Plasmid
    #165544
    Purpose
    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T5 DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165544-plasmid-map-sequence-id-339280

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  17. pWS175

    Plasmid
    #90962
    Purpose
    Cas9 gap repair vector - HygR
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90962-plasmid-map-sequence-id-287209

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  26. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  27. mEos3.2-TOMM20-N-10

    Plasmid
    #57483
    Purpose
    Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pcDNA3-neo-cterminal-3HA

    Plasmid
    #102643
    Purpose
    To generate three copies of the HA epitope tag on cDNAs.
    Depositor
    Michael E. Wright
    Type
    Empty backbone
    Tags
    3x HA (YPYDVPDYA)
    Expression
    Mammalian
    Available Since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    102643-plasmid-map-sequence-id-196911

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  44. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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Showing: 3501 - 3550 of 169365 results