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163,517 results

Showing: 4151 - 4200 of 163517 results

  1. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187454
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187454-plasmid-map-sequence-id-367517

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  5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

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  18. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from NuƱez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Article
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  24. TRAV25

    Plasmid
    #237982
    Purpose
    Encodes TRAV25 allele, P2A, and TRBC to generate TCRs via cloning
    Depositor
    Michael Birnbaum
    Insert
    TRBC-P2A-TRAV25 (TRAV25 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    237982-plasmid-map-sequence-id-478936

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  25. pAAVLINK-EF1alpha-5' KDM5C

    Plasmid
    #236670
    Purpose
    5' AAVLINK plasmid for KDM5C expression
    Depositor
    Zhonghua Lu
    Insert
    KDM5C (KDM5C Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    Promoter
    EF1alpha
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236670-plasmid-map-sequence-id-476891

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  27. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid
    #228663
    Purpose
    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)
    Depositor
    Frank von Delft, ASAP Discovery
    Insert
    Zika virus portion of NS2B protease coexpressed with NS3 protease
    Use
    Tags
    His-GST-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228663-plasmid-map-sequence-id-461604

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  33. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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  34. pDM053

    Plasmid
    #216815
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Use
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216815-plasmid-map-sequence-id-429382

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  35. pDM071

    Plasmid
    #216817
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Use
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216817-plasmid-map-sequence-id-430145

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  36. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220363-plasmid-map-sequence-id-436888

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  39. human KANK2 Ankyrin Repeats pET151

    Plasmid
    #164054
    Purpose
    Expresses human KANK2 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164054-plasmid-map-sequence-id-327671

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  45. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid
    #79649
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79649-plasmid-map-sequence-id-175067

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  46. pPD95_75

    Plasmid
    #1494
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSLā€¦
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1494-plasmid-map-sequence-id-372920

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  50. pAAV-syn-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234438
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Has Service
    AAV1
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234438-plasmid-map-sequence-id-465745

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Showing: 4151 - 4200 of 163517 results